Abstract:
:Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and lasted for a week. Double immunofluorescence microscopy revealed that the infiltrating GA1-positive cells were all positive for Ly-5, and partially positive for Thy-1.2, but negative for Mac-1, Ia, mu-chain, Lyt-1, Lyt-2, L3T4, and Fc receptor II. They contained neither peroxidase nor nonspecific esterase. The infiltrating cells thus markedly resembled natural killer (NK) cells in their cytochemical characteristics and surface markers. DNase and RNase destroyed the GA1-positive cell-inducing activity of MY-1. These results indicate that the nucleic acid components of MY-1 are responsible for this effect.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Kuramoto E,Toizumi S,Shimada S,Tokunaga Tdoi
10.1111/j.1348-0421.1989.tb00980.xsubject
Has Abstractpub_date
1989-01-01 00:00:00pages
929-40issue
11eissn
0385-5600issn
1348-0421journal_volume
33pub_type
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