Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system.

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) an...

journal_title：Microbiology and immunology

authors： Shimizu T,Ohtani K,Ba-Thein W,Inui S,Nakamura S,Hayashi H

更新日期：1996-01-01 00:00:00

abstract：:The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric ...

journal_title：Microbiology and immunology

authors： Damodharan S,Gujar R,Pattabiraman S,Nesakumar M,Hanna LE,Vadakkuppattu RD,Usha R

更新日期：2013-05-01 00:00:00

abstract：:Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Lamin...

journal_title：Microbiology and immunology

authors： Li Z,Cui B,Liu X,Wang L,Xian Q,Lu Z,Liu S,Cao Y,Zhao Y

更新日期：2020-03-01 00:00:00

abstract：:Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus-infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected ...

journal_title：Microbiology and immunology

authors： Suenaga T,Kohyama M,Hirayasu K,Arase H

更新日期：2014-09-01 00:00:00

abstract：:Murine γδ T cells develop as the first T-cell lineage within the fetal thymus and disproportionately localize in mucosal tissues such as lung, skin, uterus, and intestine of adult mice. These unique developmental features and distribution patterns of γδ T cells enable rapid functioning against various insults from pat...

journal_title：Microbiology and immunology

authors： Shibata K

更新日期：2012-04-01 00:00:00

abstract：:Bacteria of Stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (CF) patients, usually following P. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. In this study, various S. maltophilia strains were ...

journal_title：Microbiology and immunology

authors： De Vidipó LA,De Marques EA,Puchelle E,Plotkowski MC

更新日期：2001-01-01 00:00:00

abstract：:A total of 55 strains of Clostridium sordellii, 21 lethal toxin-positive and 34 lethal toxin-negative, were tested for cytotoxin production in brain heart infusion medium supplemented with 0.2% Na2HPO4 (m-BHI) and cooked-meat-glucose (CMG) medium using baby hamster kidney (BHK-21/WI-2) cells as indicator cells. The m-...

journal_title：Microbiology and immunology

authors： Nakamura S,Tanabe N,Yamakawa K,Nishida S

更新日期：1983-01-01 00:00:00

abstract：:Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the ...

journal_title：Microbiology and immunology

authors： Hirose K,Tamura K,Watanabe H

更新日期：2003-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00

abstract：:The purpose of this study was to clarify the taxonomic relationship between all the serovars and species of the genus Erysipelothrix by performing DNA-DNA hybridization experiments, the customary criterion for separation of bacterial genospecies. A total of 93 strains were isolated from a wide variety of sources, incl...

journal_title：Microbiology and immunology

authors： Takahashi T,Fujisawa T,Umeno A,Kozasa T,Yamamoto K,Sawada T

更新日期：2008-10-01 00:00:00

abstract：:Human monocytes, human peritoneal macrophages, mouse peritoneal macrophages and human peripheral neutrophils pretreated with beta-1,3-D-polyglucose derivatives showed pronounced bactericidal capacity to Escherichia coli compared to control cells. The increased bactericidal capacity was detectable in mononuclear phagoc...

journal_title：Microbiology and immunology

authors： Rasmussen Busund LT,Konopski Z,Oian P,Seljelid R

更新日期：1992-01-01 00:00:00

abstract：:Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been ...

journal_title：Microbiology and immunology

authors： Hatch WO,Scalarone GM

更新日期：2013-11-01 00:00:00

abstract：:Both cluster of differentiation (CD)4(+) and CD8(+) T lymphocytes play key roles in immunity to Brucella, in part because they secrete interferon (IFN)-γ and activate bactericidal functions in macrophages. Therefore, use of markers of macrophage activation may have diagnostic and prognostic significance. High-mobili...

journal_title：Microbiology and immunology

authors： Ayarcı AO,Yılmaz E,Sığırlı D,Budak F,Göral G,Oral HB

更新日期：2013-02-01 00:00:00

abstract：:A high molecular weight proteinaceous factor in the cell extract of sarcoma 180 (S-180) was found to inhibit phorbol myristate acetate (PMA)-triggering of macrophage H2O2 release. This factor (S-180 factor) was stable at 56 C for 1 hr and resistant to ultraviolet-irradiation. The S-180 factor inhibited the specific bi...

journal_title：Microbiology and immunology

authors： Tomioka H,Saito H

更新日期：1985-01-01 00:00:00

abstract：:Interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and other hematopoietic cells. To elucidate IL-5-mediated intracellular mechanisms, we have established IL-5-dependent and -independent murine early B cell lines, J6 and MJ88-1, respect...

journal_title：Microbiology and immunology

authors： Migita M,Yamaguchi N,Katoh S,Mita S,Matsumoto R,Sonoda E,Tsuchiya H,Matsuda I,Tominaga A,Takatsu K

更新日期：1990-01-01 00:00:00

abstract：:Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera fro...

journal_title：Microbiology and immunology

authors： Ohishi K,Kishida N,Ninomiya A,Kida H,Takada Y,Miyazaki N,Boltunov AN,Maruyama T

更新日期：2004-01-01 00:00:00

abstract：:Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

journal_title：Microbiology and immunology

authors： Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

更新日期：2000-01-01 00:00:00

abstract：:Polar monotrichous flagella (M-flagella) of Vibrio parahaemolyticus have antigens in common with those of various species of Vibrio including V. cholerae and V. anguillarum, and of Beneckea, revealed by gel diffusion tests with flagelli monomers. However, antiserum against M-flagellin of V. parahaemolyticus did not ag...

journal_title：Microbiology and immunology

authors： Shinoda S,Senoh T,Asano K,Nakahara N,Ono B

更新日期：1980-01-01 00:00:00

abstract：:A leukocidin was isolated and purified from autolysates of Pseudomonas aeruginosa strain 158 by a combination of procedures such as column chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-100, and zone electrophoresis on pevikon. The purified preparation showed a single band in each experiment using ...

journal_title：Microbiology and immunology

authors： Hirayama T,Kato I,Matsuda F,Noda M

更新日期：1983-01-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) is the causative agent of botulism in humans and animals. Only BoNT serotype A subtype 1 (BoNT/A1) is used clinically because of its high potency and long duration of action. BoNT/A1 and BoNT/A subtype 2 (BoNT/A2) have a high degree of amino acid sequence similarity in the light chain (LC) ...

journal_title：Microbiology and immunology

authors： Kohda T,Tsukamoto K,Torii Y,Kozaki S,Mukamoto M

更新日期：2020-07-01 00:00:00

abstract：:The possible presence of Paul-Bunnell (PB) antigen on Epstein-Barr virus (EBV)-transformed lymphocytes were investigated. Of 23 EBV genome-positive lymphoblastoid cell lines tested all but one absorbed PB type antibody from the serum of an infectious mononucleosis patient. The one EBV-negative B cell line tested did n...

journal_title：Microbiology and immunology

authors： Maeda M,Sairenji T,Hinuma Y

更新日期：1979-01-01 00:00:00

abstract：:A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...

journal_title：Microbiology and immunology

authors： Matsuyama M,Sugamura K,Hinuma Y

更新日期：1987-01-01 00:00:00

abstract：:Soil specimens obtained from several areas of Japan, which are closely located to or facing the Continental land of China, were examined for the distribution of Clostridium botulinum, especially pertaining to types A and B. A total of 266 specimens of Japan, when cultured, showed no type A or B toxicity, although 30 (...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Nishida S,Yoshimura K,Yu H,Lu DY,Nakamura S

更新日期：1988-01-01 00:00:00

abstract：:Sodium vanadate enhanced Newcastle disease virus (NDV)-induced cell fusion in L cells, and there was a direct correlation between the degree of cell fusion and the dose of vanadate added. When anti-F protein of NDV monospecific antiserum was added to the culture fluid of L cells infected with NDV, the enhancement of c...

journal_title：Microbiology and immunology

authors： Yamamoto F,Fujioka H,Iinuma M,Takano M,Maeno K,Nagai Y,Ito Y

更新日期：1984-01-01 00:00:00

abstract：:SCG is a major 6-branched 1,3-beta-D-glucan in Sparassis crispa Fr. SCG shows antitumor activity and also enhances the hematopoietic response in cyclophosphamide (CY)-treated mice. In the present study, the molecular mechanism of the enhancement of the hematopoietic response was investigated. The levels of interferon-...

journal_title：Microbiology and immunology

authors： Harada T,Kawaminami H,Miura NN,Adachi Y,Nakajima M,Yadomae T,Ohno N

更新日期：2006-01-01 00:00:00

abstract：:Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...

journal_title：Microbiology and immunology

authors： Chosa T,Yamamoto N,Koyanagi Y,Kohno M,Nagata K,Hinuma Y

更新日期：1984-01-01 00:00:00

abstract：:Aged C57BL/6 (B6) mice could reject allogeneic BALB/c RL male 1 tumor as efficiently as young B6 mice. However, in vitro analysis showed impaired generation of cytotoxic T cell response in aged B6 mice against allogeneic tumor. The reaction could be augmented by the addition of recombinant interleukin-2 (rIL-2). Enzym...

journal_title：Microbiology and immunology

authors： Win S,Uenaka A,Nakayama E

更新日期：2002-01-01 00:00:00

abstract：:There was a significant amount of non-specific, but not of allergen (e.g., papain, mite feces and four kinds of pollen)-specific, IgE antibodies (Abs) in the sera of normal mice. An i.n. injection of each allergen without adjuvant into mice caused an increase in total IgE Ab titers with a similar time course in the se...

journal_title：Microbiology and immunology

authors： Hannya N,Ogita-Nakanishi H,Kato R,Ijiri Y,Hayashi T,Tanaka K,Kawata R,Takenaka H,Kubota T,Yoshida R

更新日期：2018-02-01 00:00:00

abstract：:This study proposes a novel chemiluminescent assay of bacterial activity. Luminol chemiluminescence (LC) was amplified on addition of menadione to Escherichia coli suspension, and it was effectively inhibited by addition of superoxide dismutase rather than catalase. This fact suggests that H2O2 produced from O2 by sup...

journal_title：Microbiology and immunology

authors： Yamashoji S,Manome I,Ikedo M

更新日期：2001-01-01 00:00:00