Crystallization and some properties of leukocidin from Pseudomonas aeruginosa.

abstract：:Deep-seated trichosporonosis caused by Trichosporon asahii has a high mortality rate and a very poor prognosis. New species-specific oligonucleotide primers for T. asahii were developed from a sequence analysis of rRNA genes that included the internal transcribed spacer regions. A nested PCR assay with specific primer...

journal_title：Microbiology and immunology

authors： Sugita T,Nakajima M,Ikeda R,Niki Y,Matsushima T,Shinoda T

更新日期：2001-01-01 00:00:00

abstract：:Scanning electron micrograph of HeLa S3 monolayered cells, inoculated with viable bacteria of a Salmonella typhi strain GIFU 10007, revealed that the extended microvilli tangled the bacteria within 10 min after inoculation. The micrographs of HeLa cells, at 1 hr after inoculation, indicate the following: shortening of...

journal_title：Microbiology and immunology

authors： Yokoyama H,Ikedo M,Kohbata S,Ezaki T,Yabuuchi E

更新日期：1987-01-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00

abstract：:There is a deficiency in the data concerning the role of hMPV in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hMPV in adults in our region. In the present study the association of hMPV with varieties of lower respiratory tract disorders in imm...

journal_title：Microbiology and immunology

authors： El Sayed Zaki M,Raafat D,El-Metaal AA,Ismail M

更新日期：2009-11-01 00:00:00

abstract：:The effects of LV in two different species, CD-1 mice, without a genetic disposition for diabetes, and BB rats prone to T1D were examined. Male CD-1 mice that had been exposed to LV in utero developed a type 2-like diabetes with increased blood glucose, insulin levels and epididymal fat at the age of 10-15 weeks. Comb...

journal_title：Microbiology and immunology

authors： Holmberg R,Klitz W,Blixt M,Berggren PO,Juntti-Berggren L,Niklasson B

更新日期：2009-10-01 00:00:00

abstract：:The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosupp...

journal_title：Microbiology and immunology

authors： Sakwiwatkul K,Li RL,Song XM,Hu SH

更新日期：2010-01-01 00:00:00

abstract：:gp96 plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, and cross-priming, and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. The remarkable conservation of essential immunological prope...

journal_title：Microbiology and immunology

authors： Cho NH,Choi CY,Seong SY

更新日期：2004-01-01 00:00:00

abstract：:Trehalose 6,6'-dimycolate (TDM), or cord factor, is a crucial stimulus of immune responses during Mycobacterium tuberculosis infection. Although TDM has immuno-stimulatory properties, including adjuvant activity and the ability to induce granuloma formation, the mechanisms underlying these remain unknown. We hypothesi...

journal_title：Microbiology and immunology

authors： Lee WB,Yan JJ,Kang JS,Chung S,Kim LK

更新日期：2017-12-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...

journal_title：Microbiology and immunology

authors： Otsuka N,Yoshino S,Kawano K,Toyoizumi-Ajisaka H,Shibayama K,Kamachi K

更新日期：2012-07-01 00:00:00

abstract：:In malignant B lymphoma cells interleukin-10 (IL-10) expression is frequently upregulated. This effect is thought to support to the malignant transformation of these cells and to be a potential target for pharmacotherapy. To define better the mechanism for upregulation of the IL-10 gene, we tested the association betw...

journal_title：Microbiology and immunology

authors： Horie K,Ohashi M,Satoh Y,Sairenji T

更新日期：2007-01-01 00:00:00

abstract：:The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...

journal_title：Microbiology and immunology

authors： Nishimura H,Sugawara K,Gao P,Muraki Y,Hongo S,Kitame F,Nakamura K

更新日期：1995-01-01 00:00:00

abstract：:Human monocytes, human peritoneal macrophages, mouse peritoneal macrophages and human peripheral neutrophils pretreated with beta-1,3-D-polyglucose derivatives showed pronounced bactericidal capacity to Escherichia coli compared to control cells. The increased bactericidal capacity was detectable in mononuclear phagoc...

journal_title：Microbiology and immunology

authors： Rasmussen Busund LT,Konopski Z,Oian P,Seljelid R

更新日期：1992-01-01 00:00:00

abstract：:We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV-induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric as...

journal_title：Microbiology and immunology

authors： Kodama E,Igarashi A,Mori S,Hashimoto K,Suzuki T,De Clercq E,Shigeta S

更新日期：1996-01-01 00:00:00

abstract：:Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and laste...

journal_title：Microbiology and immunology

authors： Kuramoto E,Toizumi S,Shimada S,Tokunaga T

更新日期：1989-01-01 00:00:00

abstract：:We examined the effects of microspheres as antigen carriers in mucosal immunization. Shiga toxin B subunits (Stx1B) were adsorbed on 6 mum polystyrene microspheres, which were then intranasally administered to mice together with cholera toxin (CT). Stx1B-specific serum IgG production and secretory IgA production at lo...

journal_title：Microbiology and immunology

authors： Kurohane K,Kobayashi C,Imai Y

更新日期：2005-01-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:The aim of this study was to investigate the pathogenesis of Mycoplasma pneumoniae (MP) infection and its association with cardiac and hepatic damage. Between March 2013 and March 2014, 59 children with MP pneumonia (MPP) and 30 healthy children were enrolled. Serum titers of TLR4, T cell immunoglobulin and mucin-doma...

journal_title：Microbiology and immunology

authors： Fan Q,Meng J,Li P,Liu Z,Sun Y,Yan P

更新日期：2015-07-01 00:00:00

abstract：:A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...

journal_title：Microbiology and immunology

authors： Ohyama T,Watanabe T,Fujinaga Y,Inoue K,Sunagawa H,Fujii N,Inoue K,Oguma K

更新日期：1995-01-01 00:00:00

abstract：:Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet-activator-factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS als...

journal_title：Microbiology and immunology

authors： Isogai E,Hirose K,Kimura K,Hayashi S,Kubota T,Fujii N,Isogai H

更新日期：1997-01-01 00:00:00

abstract：:We developed an effective system to eliminate poliovirus from modified tap water using a positively-charged carbon felt electrode. The zeta potential of polioviruses was measured using laser microscopic electrophoresis. Poliovirus adsorption to the electrode was examined by indirect immunofluorescence. The tissue cult...

journal_title：Microbiology and immunology

authors： Kondo Y,Morita Y,Yamada A,Kimura H

更新日期：2004-01-01 00:00:00

abstract：:Mice primed with 1 microgram of reduced and alkylated ovalbumin (RA-OA) developed not only long-lived memory cells for delayed-type hypersensitivity (DTH), capable of differentiating into DTH-effector T cells (DTH-Te) against ovalbumin (OA) when restimulated in vitro with OA, but also spleen cells capable of augmentin...

journal_title：Microbiology and immunology

authors： Kojima A,Tamura SI,Egashira Y

更新日期：1982-01-01 00:00:00

abstract：:We found that a sub-lethal concentration of hydrogen peroxide (HPOx) enhanced the growth of Helicobacter pylori in Brucella broth supplemented with 10% fetal bovine serum (BB/FBS). The enhancement was evident at 0.1 mM HPOx and reached a maximun at 3.5 mM. The growth stimulation was dependent on the basal media used; ...

journal_title：Microbiology and immunology

authors： Murano A,Miyake M,Kato J,Tanzawa H,Takeo K,Noda M

更新日期：1999-01-01 00:00:00

abstract：:Distribution of indigenous lactobacilli in the gastrointestinal tracts of rats was investigated at the species level. The indigenous lactobacilli isolated from conventional rats were divided into three groups, Lactobacillus acidophilus and its related strains, L. fermentum, and L. murini. Localization of the Lactobaci...

journal_title：Microbiology and immunology

authors： Watanabe T,Morotomi M,Suegara N,Kawai Y,Mutai M

更新日期：1977-01-01 00:00:00

abstract：:Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

journal_title：Microbiology and immunology

authors： Ikigai H,Otsuru H,Yamamoto K,Shimamura T

更新日期：2006-01-01 00:00:00

abstract：:In recent years, phosphatidylinositol 4-kinase III beta (PI4KB) has emerged as a conserved target of anti-picornavirus compounds. In the present study, PI4KB was identified as the direct target of the plant-derived anti-picornavirus compounds, oxoglaucine and pachypodol (also known as Ro 09-0179). PI4KB was also ident...

journal_title：Microbiology and immunology

authors： Arita M,Philipov S,Galabov AS

更新日期：2015-06-01 00:00:00

abstract：:Structural studies were carried out on an O-antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of D-glucose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine in a molar ratio of 1:2:1:...

journal_title：Microbiology and immunology

authors： Matsuo K,Isogai E,Araki Y

更新日期：2001-01-01 00:00:00

abstract：:The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

journal_title：Microbiology and immunology

authors： Hirai K,Une T

更新日期：1986-01-01 00:00:00

abstract：:An immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of Chlamydia psittaci and infected cells using a number of monoclonal antibodies (MAbs). Of three anti-lipopolysaccharide (LPS) antibodies (4D5, A2 and 4G5), two antibodies (4D5 and A2) reacted with the...

journal_title：Microbiology and immunology

authors： Ando S,Takashima I,Hashimoto N

更新日期：1992-01-01 00:00:00

abstract：:Three strains TKU9, TKU49 and TKU50(T) , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infa...

journal_title：Microbiology and immunology

authors： Okamoto M,Imai S,Miyanohara M,Saito W,Momoi Y,Nomura Y,Ikawa T,Ogawa T,Miyabe-Nishiwaki T,Kaneko A,Watanabe A,Watanabe S,Hayashi M,Tomonaga M,Hanada N

更新日期：2015-09-01 00:00:00

abstract：:Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the ...

journal_title：Microbiology and immunology

authors： Hirose K,Tamura K,Watanabe H

更新日期：2003-01-01 00:00:00