Abstract:
:An immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of Chlamydia psittaci and infected cells using a number of monoclonal antibodies (MAbs). Of three anti-lipopolysaccharide (LPS) antibodies (4D5, A2 and 4G5), two antibodies (4D5 and A2) reacted with the surface of reticulate bodies (RBs) but not with that of elementary bodies (EBs). The other antibody (4G5) reacted with both EBs and RBs. Examination of infected cells in thin sections revealed that 4D5 and A2 combined with the membranes of both EBs and RBs. These results indicate that each LPS epitope localized at a different position in the chlamydial membrane. Most MAbs directed to protein antigens reacted on the surface of both EBs and RBs though 3E9 specific for the 90 kDa and 50 kDa protein components combined with RBs only.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Ando S,Takashima I,Hashimoto Ndoi
10.1111/j.1348-0421.1992.tb01648.xkeywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
113-21issue
2eissn
0385-5600issn
1348-0421journal_volume
36pub_type
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