Abstract:
:Because cytotoxic T lymphocytes (CTLs) play an important role in the specific immunotherapy of hepatitis C virus (HCV) infection, a series of CTL epitopes has been defined from HCV genotype 1a or 1b protein. Here, we attempted to identify HCV2a-derived epitopes that are capable of inducing HLA-A2-restricted and peptide-specific CTLs. Peripheral blood mononuclear cells (PBMCs) of HLA-A2+ HCV2ainfected patients or healthy donors were stimulated in vitro with each of the HCV2a-derived peptides, which were prepared based on the HLA-A2-binding motif, and their peptide-specific and HLA-A2-restricted cytotoxicities were examined. The HCV2a 432-441, HCV2a 716-724, and HCV2a 2251-2260 peptides were found to efficiently induce peptide-specific CTLs from the PBMCs of HLA-A2+ HCV2ainfected patients. Cytotoxicity was mainly mediated by CD8+ T cells in a HLA class I-restricted manner. These results indicate that the HCV2a 432-441, HCV2a 716-724, and HCV2a 2251-2260 peptides might be applicable for peptide-based immunotherapy of HLA-A2+ HCV2a-infected patients.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Wang Y,Takao Y,Harada M,Yutani S,Ide T,Sata M,Itoh K,Yamada Adoi
10.1111/j.1348-0421.2006.tb03861.xsubject
Has Abstractpub_date
2006-01-01 00:00:00pages
857-65issue
11eissn
0385-5600issn
1348-0421pii
JST.JSTAGE/mandi/50.857journal_volume
50pub_type
杂志文章abstract::The biological activities of lipopolysaccharide-like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton by the hot phenol-water method were studied in mice. The addition of 12.5 micrograms/ml or more of LLS fraction increased the incorporation of [3H]thymidine into in vitro cultured ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03134.x
更新日期:1987-01-01 00:00:00
abstract::Bordetella pertussis causes whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re-emergence of ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12504
更新日期:2017-09-01 00:00:00
abstract::The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1992.tb02060.x
更新日期:1992-01-01 00:00:00
abstract::Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02440.x
更新日期:1999-01-01 00:00:00
abstract::Twelve monoclonal antibodies against Negishi virus were obtained and characterized by hemagglutination inhibition (HI) and neutralization (NT) tests using five flaviviruses isolated in the pan-Pacific region. The reaction pattern of the antibodies showed that Negishi virus was most closely related to Langat virus, fol...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00758.x
更新日期:1984-01-01 00:00:00
abstract::We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2001.tb02655.x
更新日期:2001-01-01 00:00:00
abstract::Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Lamin...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12763
更新日期:2020-03-01 00:00:00
abstract::Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumo...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12386
更新日期:2016-06-01 00:00:00
abstract::The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...
journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.2008.00008.x
更新日期:2008-05-01 00:00:00
abstract::Specific tumor microenvironment signaling might prevent the maturation of dendritic cells (DCs) with tolerogenic and immunosuppressive potential accounting for antigen-specific unresponsiveness in the lymphoid organs and in the periphery. In the present study, dendritic cells treated with LLC lung cancer cell or 4T1 b...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12747
更新日期:2020-01-01 00:00:00
abstract::Methylcellulose-elicited peritoneal macrophages from BALB/c mice were cultivated in vitro and inoculated with dengue virus (DV). At intervals thereafter portions of the culture fluids were taken and titrated for viral infectivity. Extracts from Ascaris suum and Parascaris equorum, either crude or Sephadex G-100 fracti...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00832.x
更新日期:1985-01-01 00:00:00
abstract::Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reac...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1978.tb00425.x
更新日期:1978-01-01 00:00:00
abstract::A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2011.00406.x
更新日期:2012-02-01 00:00:00
abstract::A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb02229.x
更新日期:1995-01-01 00:00:00
abstract::The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb01351.x
更新日期:1987-01-01 00:00:00
abstract::ELISA was used to measure the amount and avidity of IgG antibodies to exotoxin A (ExA) and 7 Fisher's immunotypes of lipopolysaccharide (LPS) in the sera of 13 patients with mild or moderate Pseudomonas aeruginosa infections. Changes in the specificity of tested sera during the course of infection were demonstrated. A...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1991.tb01574.x
更新日期:1991-01-01 00:00:00
abstract::The function of MHC class II HLA-DR molecules expressed on a human B lymphoid progenitor cell line FL8.2.4.4 (abbreviated as FL4.4) was examined. FL4.4 cells expressed HLA-DR molecules and stimulation of the DR molecules by anti-DR mAb or by superantigen TSST-1 induced strong augmentation of homocytic aggregation and ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb02154.x
更新日期:1994-01-01 00:00:00
abstract::Dengue virus multiplication in cultures of a murine myelomonocytic cell line (WEHI-3) as well as mouse peritoneal macrophages was enhanced by treatment of the cells with lipophilic derivatives of muramyl peptides for 2 or 3 days before virus inoculation, but not for 2 hr before virus inoculation or during the adsorpti...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00855.x
更新日期:1985-01-01 00:00:00
abstract::Autoantibodies to myeloperoxidase (MPO) are a subset of anti-neutrophil cytoplasmic antibody (ANCA, MPO-ANCA) detected in the sera of some patients with primary systemic vasculitis. The titer of MPO-ANCA does not always reflect disease activity and this inconsistency may be attributable to differences in epitopic spec...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb04017.x
更新日期:2007-01-01 00:00:00
abstract::A new type of immuno-cell therapy called BRM-activated killer (BAK) therapy using non-MHC-restricted lymphocytes, CD56-positive cells, was devised. Peripheral blood lymphocytes were selected by immobilization with anti-CD3 monoclonal antibody and cultured for 2 weeks in the presence of IL-2. Thereafter, they were reac...
journal_title:Microbiology and immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1348-0421.2001.tb02638.x
更新日期:2001-01-01 00:00:00
abstract::A mouse model for the study of postexposure prophylaxis of rabies was established. Mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1980.tb02888.x
更新日期:1980-01-01 00:00:00
abstract::Human metapneumovirus (hMPV) is one of the etiological agents of acute respiratory tract infections. From June 2005 to May 2006, we collected 185 clinical specimens from children in Osaka City, Japan, and detected 41 hMPV RNA. Of the 41 specimens, four (9.8%) also contained other viruses (3 with adenovirus [AdV] and 1...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb03956.x
更新日期:2007-01-01 00:00:00
abstract::Sera from 3,158 individuals living in northern Egypt were tested for the presence of antibodies against human T-lymphotropic virus type I (HTLV-I) by the newly developed particle agglutination (PA) test. Ten sera gave a positive reaction in the PA test. Eight of these sera were examined further by Western blotting and...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01461.x
更新日期:1988-01-01 00:00:00
abstract::In the present study, 12 patients with fever of undetermined origin, anemia and icterus were diagnosed with hemoplasma infection by light microscopy, transmission electron microscopy examination and PCR assay after being excluded from other usual febrile diseases. Complement receptor type I (CR1, CD35) expression on t...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2010.00240.x
更新日期:2010-08-01 00:00:00
abstract::A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb03354.x
更新日期:1999-01-01 00:00:00
abstract::CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01091.x
更新日期:1996-01-01 00:00:00
abstract::Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03280.x
更新日期:1995-01-01 00:00:00
abstract::The 16S toxin was purified from a Clostridium botulinum type D strain 1873 (D-1873). Furthermore, the entire nucleotide sequences of the genes coding for the 16S toxin were determined. It became clear that the purified D-1873 16S toxin consists of neurotoxin, nontoxic nonhemagglutinin (NTNH), and hemagglutinin (HA), a...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02330.x
更新日期:1998-01-01 00:00:00
abstract::Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utiliz...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01212.x
更新日期:1997-01-01 00:00:00
abstract::A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP c...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02327.x
更新日期:1998-01-01 00:00:00