Antifungal activity of Rubus chingii extract combined with fluconazole against fluconazole-resistant Candida albicans.

Abstract:

:This study aimed to investigate the antifungal activity of Rubus chingii extract in combination with fluconazole (FLC) against FLC-resistant Candida albicans 100 in vitro. A R. chingii extract and FLC-resistant C. albicans fungus suspension were prepared. The minimum inhibitory concentration and fractional inhibitory concentration index of R. chingii extract combined with FLC against C. albicans were determined, after which growth curves for C. albicans treated with R. chingii extract, FLC alone and a combination of these preparations were constructed. Additionally, the mechanisms of drug combination against C. albicans were explored by flow cytometry, gas chromatographic mass spectrometry and drug efflux pump function detection. R. chingii extract combined with FLC showed significant synergy. Flow cytometry suggested that C. albicans cells mainly arrest in G1 and S phases when they have been treated with the drug combination. The drug combination resulted in a marked decrease in the ergosterol content of the cell membrane. Additionally, efflux of Rhodamine 6G decreased with increasing concentrations of R. chingii extract. R. chingii extract combined with FLC has antifungal activity against FLC-resistant C. albicans.

journal_name

Microbiol Immunol

authors

Han B,Chen J,Yu YQ,Cao YB,Jiang YY

doi

10.1111/1348-0421.12357

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

82-92

issue

2

eissn

0385-5600

issn

1348-0421

journal_volume

60

pub_type

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