Abstract:
:A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP(MARQ), PrP(TARQ) and PrP(MARR), were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171-Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie-resistant progenitors in the flocks.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Ishiguro N,Shinagawa M,Onoe S,Yamanouchi K,Saito Tdoi
10.1111/j.1348-0421.1998.tb02327.xsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
579-82issue
8eissn
0385-5600issn
1348-0421journal_volume
42pub_type
杂志文章abstract::Herpes simplex virus type 1 (HSV-1) was reactivated more rapidly in cells of latently infected mouse trigeminal ganglia which were cultured in serum-free medium (after 3.7 days of cultivation) than in those cultured in serum-containing Dulbecco's modified Eagle's medium (after 8.5 days of cultivation). The concentrati...
journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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