Epitope analysis using anti-oligosaccharide (G4) monoclonal antibody.

Abstract:

:A murine monoclonal antibody recognizing (1-->6)-beta-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyllan, an antitumor polysaccharide, but notable reactivity with some low-molecular-weight schizophyllans. This antibody was useful for determination of the epitope of several polysaccharides. The extent of reactivity of this monoclonal antibody was related only to the molecular weight of schizophyllan.

journal_name

Microbiol Immunol

authors

Hirata A,Komoda M,Itoh W,Tabata K,Sugawara I

doi

10.1111/j.1348-0421.1995.tb02166.x

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

43-7

issue

1

eissn

0385-5600

issn

1348-0421

journal_volume

39

pub_type

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