Alterations of CD4(+) CD25(+) Foxp3(+) regulatory T cells in HIV-infected slow progressors of former blood donors in China.


:Our objective was to study the alterations of CD4(+) CD25(+) Foxp3(+) T(regs) in HIV-infected SPs and to examine the role of T(regs) in the disease progression of HIV. The proportion of CD4(+) CD25(+) Foxp3(+) T(regs) in peripheral blood of 24 SPs, 30 asymptomatic HIV-infected patients, 20 AIDS patients, and 16 non-infected controls was quantified using flow cytometry. HIV Gag peptide mix-induced IFN-γ expression in CD8(+) T cells in whole and CD25-depleted PBMCs was examined to evaluate the function of T(regs) . The expression of CTLA-4 in T(regs) was also detected to measure the suppressive effect of T(regs) . HLA-DR and CD38 expression were measured to study the relationship between the frequency of T(regs) and immune activation of HIV-infected patients. The frequency of CD4(+) CD25(+) Foxp3(+) regulatory T cells in SPs was lower than in asymptomatic HIV-infected patients, AIDS patients, and normal controls (P < 0.05). T(regs) in SPs showed lower intracellular CTLA-4 expression than those of asymptomatic HIV-infected patients and AIDS patients (P < 0.05). The frequency of T(regs) significantly correlated with the percentage of CD38 expression on CD4(+) and CD8(+) T cells (P < 0.05). Multivariate regression analysis showed that the CD4(+) T cell count was the strongest independent factor correlated with the absolute count of T(regs) , while viral load had the strongest predictive strength on the proportion of T(regs) . We conclude that a lower frequency of T(regs) and intracellular CTLA-4 expression of T(regs) was one of the characteristics of SPs that may have important clinical impacts for the prediction of the clinical progress of HIV infection.


Microbiol Immunol


Zhang Z,Jiang Y,Zhang M,Liu J,Sun G,Shi W,Wang Y,Shang H




Has Abstract


2010-10-01 00:00:00












  • Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method.

    abstract::A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

    更新日期:2012-02-01 00:00:00

  • The matrix gene expression of subacute sclerosing panencephalitis (SSPE) virus (Osaka-1 strain): a comparison of two sibling viruses isolated from different lobes of an SSPE brain.

    abstract::The Fr/V and Oc/V sibling viruses of the Osaka-1 strain of the subacute sclerosing panencephalitis (SSPE) virus were defective in cell-free virus production. By radioimmunoprecipitation assay, the matrix (M) protein was not detected in cells persistently infected with the Osaka-1 strain. This undetectable expression w...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ayata M,Hayashi K,Seto T,Murata R,Ogura H

    更新日期:1998-01-01 00:00:00

  • Cloning and molecular characterization of outer membrane protein A (ompA) gene from Mycobacterium bovis.

    abstract::The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Singh JP,Verma R

    更新日期:2008-08-01 00:00:00

  • Further search for small molecular inactivants capable of eliciting respiratory mucosal immunogenicity by modifying Sendai virus core RNA.

    abstract::Five groups of 32 chemicals were examined regarding their immunological functions as modifier inactivants to make inactivated Sendai nasal vaccine using a contact exposure experiment, direct immunofluorescent method, and serum HI titer. (1) Five of the nine reactive groups of reactive dyes (2-chloropyridine, 2, 4, 6-t...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Miyamae T

    更新日期:1996-01-01 00:00:00

  • Participation of macrophages in enhanced in vitro immune interferon (IFN gamma) production with mouse spleen cells.

    abstract::IFN gamma production in cultures of spleen cells obtained from mice sensitized with TH69, a live Streptococcus faecalis preparation, was examined to determine how macrophages participate. It was demonstrated that sensitized spleen macrophages participated in enhanced IFN gamma production by T cells at an early stage (...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Yamaguchi T,Kuroda Y,Yokoyama T,Saito M,Ebina T,Hoshino F,Ishida N

    更新日期:1984-01-01 00:00:00

  • New epitope peptides derived from hepatitis C virus (HCV) 2a which have the capacity to induce cytotoxic T lymphocytes in HLA-A2+ HCV-infected patients.

    abstract::Because cytotoxic T lymphocytes (CTLs) play an important role in the specific immunotherapy of hepatitis C virus (HCV) infection, a series of CTL epitopes has been defined from HCV genotype 1a or 1b protein. Here, we attempted to identify HCV2a-derived epitopes that are capable of inducing HLA-A2-restricted and peptid...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Wang Y,Takao Y,Harada M,Yutani S,Ide T,Sata M,Itoh K,Yamada A

    更新日期:2006-01-01 00:00:00

  • Properties of T antigen-positive phenotypic revertants isolated from SV40-transformed rat and mouse cells.

    abstract::Four T antigen-positive phenotypic revertants were isolated by negative selection with BUdR from SV40-transformed rat and mouse cells which contain six and two viral genome equivalents per cell, respectively. Karyological analysis indicated that one rat and one mouse revertant had a hyperploid number of chromosomes, w...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Maruyama K,Furuta T,Ueda K,Oda K

    更新日期:1982-01-01 00:00:00

  • Effector mechanism and clinical response of BAK (BRM-activated killer) immuno-cell therapy for maintaining satisfactory QOL of advanced cancer patients utilizing CD56-positive NIE (neuro-immune-endocrine) cells.

    abstract::A new type of immuno-cell therapy called BRM-activated killer (BAK) therapy using non-MHC-restricted lymphocytes, CD56-positive cells, was devised. Peripheral blood lymphocytes were selected by immobilization with anti-CD3 monoclonal antibody and cultured for 2 weeks in the presence of IL-2. Thereafter, they were reac...

    journal_title:Microbiology and immunology

    pub_type: 临床试验,杂志文章


    authors: Ebina T,Ogama N,Shimanuki H,Kubota T,Isono N

    更新日期:2001-01-01 00:00:00

  • Cloning of hok gene into anhydrotetracycline inducible pASK75 vector reveals potent antimicrobial effect of 19 amino acid long N-terminal fragment of hok peptide.

    abstract::An important toxin-antitoxin (TA) system hok/sok, encoded by R1 plasmid of Escherichia coli, is involved in the post segregation killing of cells that have lost the plasmid. The lethal properties of hok protein have been utilized for the environmental containment of microbes and the development of potential vaccine ca...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kaur A,Muthukumarappa T,Kanta P,Banday AZ,Chidananda MK

    更新日期:2020-11-01 00:00:00

  • High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPbeta, on AZ-521.

    abstract::VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) beta, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fet...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kimura T,Wada A,Nakayama M,Ogushi K,Nishi Y,De Guzman BB,Moss J,Hirayama T

    更新日期:2003-01-01 00:00:00

  • Monoclonal antibody against Mycoplasma fermentans-specific aminoglycoglycerolipid.

    abstract::Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholine-containing glycoglycerolipids: GGPL-I and GGPL-III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF-III-1) specific to GGPL-III (phosphocholine-con...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Matsuda K,Li JL,Ichinose S,Harasawa R,Saito M,Yamamoto N

    更新日期:2000-01-01 00:00:00

  • Human T-lymphoblastoid cell lines with high and low abilities to produce interferon-gamma constitutively and their susceptibilities to interferon.

    abstract::A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Matsuyama M,Sugamura K,Hinuma Y

    更新日期:1987-01-01 00:00:00

  • Effect of the sialyl Lewis X (SLe(x)) moiety on splenic accumulation of SLe(x)-carboxymethylpullulan conjugate.

    abstract::Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CM...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Horie K,Sakagami M,Kuramochi K,Ito T,Hamana H

    更新日期:2000-01-01 00:00:00

  • Characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats.

    abstract::The aim of this study was to explore the presence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in a collection of S. pseudintermedius strains isolated from dogs and cats with dermatitis in Japan and to compare their genotypic and phenotypic characteristics. Clonal relationships were determined by pu...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Bardiau M,Yamazaki K,Ote I,Misawa N,Mainil JG

    更新日期:2013-07-01 00:00:00

  • Enhancement of DTH response by cholera toxin B subunit inoculated intranasally together with influenza HA vaccine.

    abstract::The effects of B subunit of cholera toxin (CTB) on delayed-type hypersensitivity (DTH) response to influenza vaccine derived from influenza virus A/PR/8/34 (PR-8, HlNl) virus were investigated in B10 mice that were immunized intranasally with both influenza vaccine and CTB. The result showed that intranasal inoculatio...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kikuta K,Kurata H,Nagamine T,Aizawa C,Ueno Y,Kurata T,Tamura S

    更新日期:1990-01-01 00:00:00

  • Mitogenic Activity of a Water-Soluble Adjuvant (Bu-WSA) Obtained from Bacterionema matruchotii: IV. Synergistic Effects of Bu-WSA on Concanavalin A-Induced Proliferative Response of Human Peripheral Blood Lymphocytes.

    abstract::Butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii was cultured with peripheral blood mononuclear cells (PBM) in the presence of sub- and/or supra-optimal mitogenic concentrations of concanavalin A (Con A). The addition of Bu-WSA resulted in increased tritiated thymidine incorpora...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Nitta T,Okumura S,Tsushi M,Nakano M

    更新日期:1982-07-01 00:00:00

  • A highly effective method for removing suspended poliovirus from water using a positively-charged carbon felt electrode.

    abstract::We developed an effective system to eliminate poliovirus from modified tap water using a positively-charged carbon felt electrode. The zeta potential of polioviruses was measured using laser microscopic electrophoresis. Poliovirus adsorption to the electrode was examined by indirect immunofluorescence. The tissue cult...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kondo Y,Morita Y,Yamada A,Kimura H

    更新日期:2004-01-01 00:00:00

  • Cytopathic effect of heat-labile toxin of Bordetella parapertussis on aortic smooth muscle cells from pigs or guinea pigs.

    abstract::The in vitro effect of the heat-labile toxin (HLT) of Bordetella parapertussis on HeLa, baby hamster kidney (BHK), chinese hamster ovary (CHO), myeloma (Pa-NS-1), human embryonic lung (HEL-R66) cells, erythrocytes, adipocytes and lymphocytes from guinea pigs, mice or rats, or aortic smooth muscle cells from pigs or gu...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Endoh M,Nagai M,Ueda T,Yoshida Y,Nakase Y

    更新日期:1988-01-01 00:00:00

  • Effects of cytochalasins B and D on Staphylococcus aureus adherence to and ingestion by mouse renal cells from primary culture.

    abstract::Cytochalasin B (CB) and cytochalasin D (CD), inhibitors of microfilament function of host cell, were examined for their effects on Staphylococcus aureus Cowan I adherence to and ingestion by several types of the hyperosmolarity-tolerant (HOT) cells obtained from primary culture of mouse kidney. Staphylococcal adherenc...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Murai M,Seki K,Sakurada J,Usui A,Masuda S

    更新日期:1993-01-01 00:00:00

  • Effect of disruption by sonication under different conditions on the activity of glucose dehydrogenase from resting spores of Bacillus subtilis.

    abstract::The activity of glucose dehydrogenase present in resting spores of Bacillus subtilis varied strikingly with the conditions for disrupting the spores by sonic treatment, namely, the time and strength of sonication, and the type and pH of the solution used for suspending the spores. When the resting spores were sonicate...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Tochikubo K,Yasuda Y

    更新日期:1983-01-01 00:00:00

  • MASTDISCS combi Carba plus, a simple method for discriminating carbapenemase-producing Enterobacteriaceae, including OXA-48-type producers.

    abstract::Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

    更新日期:2018-01-01 00:00:00

  • A novel combination of selective agents for isolation of Leptospira species.

    abstract::A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 μg/mL; amphotericin B, 5 μg/mL; fosfomycin, 400 μg/mL; and 5-fluorouracil, 100 μg/mL) was developed for selective isolation of leptospires from contaminated samples. The growth of 16 microorganisms considered as possible contamin...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Chakraborty A,Miyahara S,Villanueva SY,Saito M,Gloriani NG,Yoshida S

    更新日期:2011-07-01 00:00:00

  • WT1 peptide-based immunotherapy for patients with lung cancer: report of two cases.

    abstract::The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Tsuboi A,Oka Y,Osaki T,Kumagai T,Tachibana I,Hayashi S,Murakami M,Nakajima H,Elisseeva OA,Fei W,Masuda T,Yasukawa M,Oji Y,Kawakami M,Hosen N,Ikegame K,Yoshihara S,Udaka K,Nakatsuka S,Aozasa K,Kawase I,Sugiyama H

    更新日期:2004-01-01 00:00:00

  • Molecular detection of various rickettsiae in mites (acari: trombiculidae) in southern Jeolla Province, Korea.

    abstract::This study revealed the presence of various rickettsial agents in mites from wild rodents collected in Southern Jeolla Province, Korea, by nested polymerase chain reaction (PCR) and sequence analysis of a partial citrate synthase and rickettsia outer membrane protein B genes. Rickettsial agents closely related to the ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Choi YJ,Lee EM,Park JM,Lee KM,Han SH,Kim JK,Lee SH,Song HJ,Choi MS,Kim IS,Park KH,Jang WJ

    更新日期:2007-01-01 00:00:00

  • The 1st step initiation essential for allergen-specific IgE antibody production upon the 2nd step: Induction of non-specific IgE+ small B cells containing secondly-sensitized allergen-specific ones in mice firstly-sensitized with an allergen.

    abstract::There was a significant amount of non-specific, but not of allergen (e.g., papain, mite feces and four kinds of pollen)-specific, IgE antibodies (Abs) in the sera of normal mice. An i.n. injection of each allergen without adjuvant into mice caused an increase in total IgE Ab titers with a similar time course in the se...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Hannya N,Ogita-Nakanishi H,Kato R,Ijiri Y,Hayashi T,Tanaka K,Kawata R,Takenaka H,Kubota T,Yoshida R

    更新日期:2018-02-01 00:00:00

  • Intracellular localization of Staphylococcus aureus within primary cultured mouse kidney cells.

    abstract::Staphylococcus aureus Cowan I was incubated with monolayers of cells derived from several portions of mouse kidney, and found to be ingested by all types of the renal cells. Intracellular localization of S. aureus was determined by resistance of intracellular cocci against lysostaphin digestion and confirmed by electr...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Murai M,Usui A,Seki K,Sakurada J,Masuda S

    更新日期:1992-01-01 00:00:00

  • Mucosal immunoregulation: environmental lipopolysaccharide and GALT T lymphocytes regulate the IgA response.

    abstract::In this review, we have emphasized: 1) bacterial lipopolysaccharide (LPS) involvement in IgA responses to orally administered thymic-dependent (TD) antigens; 2) characterization of Peyer's patch (PP) lymphoreticular cells; and 3) gastrointestinal immunization with gram negative pathogens and anti-LPS immunity to infec...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: McGhee JR,Michalek SM,Kiyono H,Eldridge JH,Colwell DE,Williamson SI,Wannemuehler MJ,Jirillo E,Mosteller LM,Spalding DM

    更新日期:1984-01-01 00:00:00

  • Intracellular survival and innate immune evasion of Burkholderia cepacia: Improved understanding of quorum sensing-controlled virulence factors, biofilm, and inhibitors.

    abstract::Burkholderia cepacia complex (Bcc) are opportunistic pathogens implicated with nosocomial infections, and high rates of morbidity and mortality, especially in individuals with cystic fibrosis (CF). B. cepacia are naturally resistant to different classes of antibiotics, and can subvert the host innate immune responses ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: Ganesh PS,Vishnupriya S,Vadivelu J,Mariappan V,Vellasamy KM,Shankar EM

    更新日期:2020-02-01 00:00:00

  • Deletion polymorphisms in the promoter region of Fcgamma receptor IIB is not associated with antigen-specific IgG2a and IgG2b antibody responses in NC/Nga mice.

    abstract::Fcgamma receptor (R) IIB, a low-affinity FcR for IgG, inhibits B cell Ag R (BCR)-mediated activation when these two receptors are cross-linked by Ag and IgG-containing immune complexs (ICs). We found deletion polymorphisms in the promoter region of fcgr2b in NC/Nga mice, a model for human atopic dermatitis. NC/Nga mic...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Sakai T,Kogiso M,Mitsuya K,Komatsu T,Yamamoto S

    更新日期:2006-01-01 00:00:00

  • Structural requirements of cholesterol for binding to Vibrio cholerae hemolysin.

    abstract::Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ikigai H,Otsuru H,Yamamoto K,Shimamura T

    更新日期:2006-01-01 00:00:00