Stenotrophomonas maltophilia interaction with human epithelial respiratory cells in vitro.

abstract：:TtT/M-87 cell is a macrophage cell line established from thyrotropic pituitary tumor tissues in mouse. In this paper, we report the immunological properties of M-87 cells as a model of tumor-associated macrophage. Contrasting with resident peritoneal macrophages, M-87 cells constitutively secreted small but significan...

journal_title：Microbiology and immunology

authors： Mitsuishi T,Akuzawa Y,Sato S,Rui J,Kodama K,Inoue K,Kurashige S

更新日期：1993-01-01 00:00:00

abstract：:Bacterial images can be obtained rather easily with an atomic-force microscope (AFM) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. The bacterial shapes or the presence of flagella can be clearly recogni...

journal_title：Microbiology and immunology

authors： Umeda A,Saito M,Amako K

更新日期：1998-01-01 00:00:00

abstract：:A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

journal_title：Microbiology and immunology

authors： Nakatani T,Higuchi A,Noguchi H

更新日期：1992-01-01 00:00:00

abstract：:We prepared polyclonal antibody specific to Mycoplasma pneumoniae. Using this antibody, we developed a latex agglutination test (LAT) for detecting the organism in respiratory exudates as rapid diagnosis of M. pneumoniae infection. Further, LAT was compared with DNA-probe test (DP) which was the only commercially avai...

journal_title：Microbiology and immunology

authors： Masayoshi T,Hirai Y,Kanemasa Y

更新日期：1992-01-01 00:00:00

abstract：:Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholine-containing glycoglycerolipids: GGPL-I and GGPL-III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF-III-1) specific to GGPL-III (phosphocholine-con...

journal_title：Microbiology and immunology

authors： Matsuda K,Li JL,Ichinose S,Harasawa R,Saito M,Yamamoto N

更新日期：2000-01-01 00:00:00

abstract：:An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

journal_title：Microbiology and immunology

authors： Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

更新日期：2013-06-01 00:00:00

abstract：:Five groups of 32 chemicals were examined regarding their immunological functions as modifier inactivants to make inactivated Sendai nasal vaccine using a contact exposure experiment, direct immunofluorescent method, and serum HI titer. (1) Five of the nine reactive groups of reactive dyes (2-chloropyridine, 2, 4, 6-t...

journal_title：Microbiology and immunology

authors： Miyamae T

更新日期：1996-01-01 00:00:00

abstract：:Recombinant human interferon-gammas (rHuIFN-gamma s) were obtained from two different mammalian cells (mouse C127 cells and Chinese hamster ovary, CHO, cells) cultured in a microcarrier culture system. Both rHuIFN-gamma s were purified using sequential chromatographies for their comparison of structural properties. Th...

journal_title：Microbiology and immunology

authors： Sano E,Kamata K,Okano K,Yamazaki S,Iizuka M,Kobayashi S

更新日期：1988-01-01 00:00:00

abstract：:Hg- and Cd-spores of Bacillus megaterium QM B1551 were produced in Schaeffer's medium containing mercuric chloride and cadmium chloride respectively. Metals were added to the medium at 9 hr of incubation (Stage V) to give a final concentration of 50 microM. It was found by electron microscopic and biochemical studies ...

journal_title：Microbiology and immunology

authors： Imagawa M,Ichikawa T,Kondo M

更新日期：1982-01-01 00:00:00

abstract：:The mechanisms of B cell tolerance were studied in an attempt to learn whether B cells rendered tolerant are present in the immune system in a potentially responsive form. The author tested the in vitro anti-trinitrophenyl (TNP) antibody-forming cell (anti-TNP AFC) response to TNP-immunogens and polyclonal B cell acti...

journal_title：Microbiology and immunology

authors： Uchiyama T

更新日期：1980-01-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CM...

journal_title：Microbiology and immunology

authors： Horie K,Sakagami M,Kuramochi K,Ito T,Hamana H

更新日期：2000-01-01 00:00:00

abstract：:Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Iwata H,Miyadai T,Kimura Y

更新日期：1988-01-01 00:00:00

abstract：:A cholera-like enterotoxin was purified from Vibrio cholerae O139 strain AI-1841 isolated from a diarrheal patient in Bangladesh. Its characteristics were compared with that of cholera toxins (CTs) of classical strain 569B and El Tor strain KT25. Al-1841 produced as much toxin as O1 strains. The toxins were indistingu...

journal_title：Microbiology and immunology

authors： Nakashima K,Eguchi Y,Nakasone N

更新日期：1995-01-01 00:00:00

abstract：:Cytochalasin B (CB) and cytochalasin D (CD), inhibitors of microfilament function of host cell, were examined for their effects on Staphylococcus aureus Cowan I adherence to and ingestion by several types of the hyperosmolarity-tolerant (HOT) cells obtained from primary culture of mouse kidney. Staphylococcal adherenc...

journal_title：Microbiology and immunology

authors： Murai M,Seki K,Sakurada J,Usui A,Masuda S

更新日期：1993-01-01 00:00:00

abstract：:The purpose of this study was to sequence the central part of the coding region of the Clostridium tyrobutyricum fiagellin gene to improve the immunoenzymatic counting of cells after milk filtration. The coding region was amplified by PCR, and the amplified products were cloned. A DNA sequence analysis of positive clo...

journal_title：Microbiology and immunology

authors： Arnold F,Bédouet L,Batina P,Robreau G

更新日期：1999-01-01 00:00:00

abstract：:The production of heat-labile enterotoxin (LT) in 76 strains of human enterotoxigenic Escherichia coli (ETEC) varied by a factor of 100. Three ETEC strains that differ in the levels of LT production were chosen for the cloning of LT genes (toxAB) into plasmid pBR322, and the gene structure and expression were compared...

journal_title：Microbiology and immunology

authors： Katayama S,Ninomiya M,Minami J,Okabe A,Hayashi H

更新日期：1990-01-01 00:00:00

abstract：:The present study was conducted to determine the possible role of intracellular Ca2+ in lipid peroxide formation in endotoxin-poisoned mice. Leakages of LDH isozyme and acid phosphatase in serum of mice fed a Ca2+-deficient diet were remarkably increased after administration of 200 micrograms of endotoxin compared to ...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Ibata H,Yokota K

更新日期：1989-01-01 00:00:00

abstract：:A bacterial strain with affinity to Candida albicans was successfully obtained from a natural environment. An uncovered Petri dish containing a suspension of heat-killed C. albicans cells was allowed to stand in a laboratory for several days. Some bacteria which had adhered to the candidal cells were tested for their ...

journal_title：Microbiology and immunology

authors： Seki K,Kobayashi K,Tomiya Y,Sakurada J,Murai M,Usui A,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:Viable but nonculturable (VBNC) Vibrio cholerae non-O1/non-O139, V. parahaemolyticus, enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-...

journal_title：Microbiology and immunology

authors： Senoh M,Ghosh-Banerjee J,Ramamurthy T,Colwell RR,Miyoshi S,Nair GB,Takeda Y

更新日期：2012-05-01 00:00:00

abstract：:The mitogen effect on migration of eosinophils and monocytes was studied in embryonic chickens. On the 13th embryonic day, chickens were injected with mitogens, such as concanavalin A (Con A), phytohemagglutinin-P (PHA-P), and lipopolysaccharide (LPS), into the allantoic cavity, and the mitogenic effect was estimated ...

journal_title：Microbiology and immunology

authors： Moriya O,Ichikawa Y

更新日期：1979-01-01 00:00:00

abstract：:We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antise...

journal_title：Microbiology and immunology

authors： Urasawa S,Urasawa T,Taniguchi K,Morita Y,Sakurada N,Saeki Y,Morita O,Hasegawa S

更新日期：1988-01-01 00:00:00

abstract：:Butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii was cultured with peripheral blood mononuclear cells (PBM) in the presence of sub- and/or supra-optimal mitogenic concentrations of concanavalin A (Con A). The addition of Bu-WSA resulted in increased tritiated thymidine incorpora...

journal_title：Microbiology and immunology

authors： Nitta T,Okumura S,Tsushi M,Nakano M

更新日期：1982-07-01 00:00:00

abstract：:Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

journal_title：Microbiology and immunology

authors： Saburi Y,Okuda K,Takahashi T,Tadokoro I

更新日期：1977-10-20 00:00:00

abstract：:Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reac...

journal_title：Microbiology and immunology

authors： Himeno K,Nomoto K,Takeya K

更新日期：1978-01-01 00:00:00

abstract：:Measles vaccines are highly effective and safe; however, the mechanism(s) underlying their attenuation has not been well understood. In this study, type I IFNs (IFN-α and IFN-β) induction in macaques infected with measles virus (MV) strains was examined. Type I IFNs were not induced in macaques infected with wild-type...

journal_title：Microbiology and immunology

authors： Van Nguyen N,Kato SI,Nagata K,Takeuchi K

更新日期：2016-07-01 00:00:00

abstract：:One- and two-dimensional polyacrylamide gel electrophoresis (PAGE) was performed on immunoprecipitates formed between anti-human T-cell xeno-antiserum (ATS) and cell-surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a Lentil lectin-coupled Sepharose 4B column. ...

journal_title：Microbiology and immunology

authors： Fujimoto J,Ishii Y,Kon S,Matsuura A,Kikuchi K

更新日期：1981-01-01 00:00:00

abstract：:JC polyomavirus (JCPyV) causes progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system, in immunocompromised patients. Because no drugs have been approved for treating PML, many antiviral agents are currently being investigated for this purpose. The inhibitory effe...

journal_title：Microbiology and immunology

authors： Nukuzuma S,Nakamichi K,Kameoka M,Sugiura S,Nukuzuma C,Tasaki T,Takegami T

更新日期：2016-04-01 00:00:00

abstract：:Dialyzable factors (DF) were prepared from ribosomal fractions of several organisms including rough mutants of Salmonella typhimurium LT2, salmonella species of different serogroups, other enteric bacteria and gram-positive organisms, and tested for their immunogenicity against S. typhimurium infection in mice. All of...

journal_title：Microbiology and immunology

authors： Kita E,Yasui K,Yasui K,Matsuda Y,Matsuda K,Kashiba S

更新日期：1984-01-01 00:00:00

abstract：:The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17-kDa antigen gene of Rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the Katayama, Abe, Misaka and Kojima strains,...

journal_title：Microbiology and immunology

authors： Tange Y,Matsumoto M,Okada T,Hasegawa H,Kobayashi Y

更新日期：1994-01-01 00:00:00