Abstract:
:Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholine-containing glycoglycerolipids: GGPL-I and GGPL-III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF-III-1) specific to GGPL-III (phosphocholine-containing aminoglycoglycerolipid) using methods of electron microscopy, immunofluorescence cell surface staining, laser scanning microscopy, immunoelectron microscopy, and thin-layer chromatography immunostaining. The MF-III-1 antibody specifically recognized M. fermentans attached to the surface of HTLV-I-infected human helper T-cells, and it did not cross-react with other lipids nor with human T-cell antigens. Since MF-III-1 distinguishes GGPL-III from GGPL-I, the binding site may include a serinol (2-amino-1,3-propanediol) residue of GGPL-III. MF-III-1 is useful for the in vitro study of M. fermentans, and may also be useful as a tool for the study of the involvement of M. fermentans in human diseases.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Matsuda K,Li JL,Ichinose S,Harasawa R,Saito M,Yamamoto Ndoi
10.1111/j.1348-0421.2000.tb02551.xkeywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
695-702issue
8eissn
0385-5600issn
1348-0421journal_volume
44pub_type
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