Abstract:
:Heat resistance at 95 C, heat activation at 75 C, and germination response were determined for spores of 10 serotype strains of Clostridium perfringens type A, including five heat-resistant and five heat-sensitive strains. The D95-values ranged from 17.6 to 63.0 and from 1.3 to 2.8 for the heat-resistant and the heat-sensitive strains, respectively. The heat-activation values, the ratios between the heated and unheated viable counts of spore suspensions, ranged from 0.0035 to 0.65 and from 6.5 to 60.0 for the heat-sensitive and the heat-resistant strains, respectively. Spores of these strains were divided into two distinct germination types on the basis of their germination response; spores of the heat-resistant strains germinated in KC1 medium after heat activation (K-type), and spores of the heat-sensitive strains germinated in a mixture of L-alanine, inosine, and CaCl2 in the presence of CO2 without heat activation (A-type). The strains were tested for enterotoxigenicity by a reversed passive latex-agglutination (RPLA) test. All the heat-resistant strains were RPLA-positive, whereas the heat-sensitive strains were all RPLA-negative. A total of 37 strains of the organism isolated from food-poisoning outbreaks were tested for spore germination and enterotoxin formation. All of the 20 heat-resistant strains showed K-type spore germination and, except for three strains, were RPLA-positive, whereas all of the 17 heat-sensitive strains showed A-type spore germination and, except for only one strain, were RPLA-negative.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Ando Y,Tsuzuki T,Sunagawa H,Oka Sdoi
10.1111/j.1348-0421.1985.tb00830.xsubject
Has Abstractpub_date
1985-01-01 00:00:00pages
317-26issue
4eissn
0385-5600issn
1348-0421journal_volume
29pub_type
杂志文章abstract::Sodium vanadate enhanced Newcastle disease virus (NDV)-induced cell fusion in L cells, and there was a direct correlation between the degree of cell fusion and the dose of vanadate added. When anti-F protein of NDV monospecific antiserum was added to the culture fluid of L cells infected with NDV, the enhancement of c...
journal_title:Microbiology and immunology
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doi:10.1111/j.1348-0421.1984.tb02948.x
更新日期:1984-01-01 00:00:00
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doi:10.1111/j.1348-0421.1986.tb03061.x
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journal_title:Microbiology and immunology
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更新日期:1979-01-01 00:00:00
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.1984.tb00679.x
更新日期:1984-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
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更新日期:1995-01-01 00:00:00
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更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1981.tb00136.x
更新日期:1981-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1983.tb00619.x
更新日期:1983-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1979-01-01 00:00:00
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更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00