Abstract:
:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) and interferon gamma (IFN-gamma) upon antigen (Ag)-specific stimulation. Downregulation of human leukocyte antigen (HLA)-DR surface expression and HLA-DR mRNA levels in P36 cells were observed when P36 cells were infected with T. gondii. Such downregulated HLA-DR expressions of T. gondii-infected P36 cells were upregulated by treatment with both recombinant IL-6 (rIL-6) and recombinant IFN-gamma (rIFN-gamma). The antigen-presenting ability of T. gondii-infected P36 cells to T. gondii-infected cell-specific CTL was enhanced by rIFN-gamma but not by rIL-6. The present study reveals the existence of differential regulation of HLA-DR expression and Ag presentation in T. gondii-infected melanoma cells by IL-6 and IFN-gamma.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Yang TH,Aosai F,Norose K,Ueda M,Yano Adoi
10.1111/j.1348-0421.1996.tb01091.xsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
443-9issue
6eissn
0385-5600issn
1348-0421journal_volume
40pub_type
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更新日期:1994-01-01 00:00:00
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更新日期:1995-01-01 00:00:00
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更新日期:2020-02-01 00:00:00