Abstract:
:Bacterial images can be obtained rather easily with an atomic-force microscope (AFM) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. The bacterial shapes or the presence of flagella can be clearly recognized in these magnification ranges. In addition, we were also able to distinguish between gram-negative and gram-positive bacteria based on the specific wavy surface appearance of the former. AFM could thus be a useful tool for the identification of bacteria in the resolution range between electron and light microscopy.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Umeda A,Saito M,Amako Kdoi
10.1111/j.1348-0421.1998.tb02266.xsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
159-64issue
3eissn
0385-5600issn
1348-0421journal_volume
42pub_type
杂志文章abstract::Integration of bovine leukemia virus (BLV) in the genomes of infected cells was investigated in cattle with enzootic bovine leukosis (EBL) and sporadic bovine leukosis (SBL). Southern blot hybridization of BLV cDNA to Eco RI and Xba I restriction fragments of EBL tumor DNAs revealed that: 1) one to four or more copies...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1982.tb00227.x
更新日期:1982-01-01 00:00:00
abstract::For the simple and rapid detection/identification of major pathogenic fungal species such as Candida albicans, C. tropicalis, C. parapsilosis, C. glabrata and Aspergillus fumigatus, common primers for these species and specific primers for each species, designed on the basis on the genomic nucleotide sequences of the ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03426.x
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pub_type: 杂志文章
doi:10.1111/j.1348-0421.2000.tb02581.x
更新日期:2000-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1979-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02298.x
更新日期:1998-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02427.x
更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2001.tb02644.x
更新日期:2001-01-01 00:00:00
abstract::We analyzed the mechanism of spontaneous B cell activation in lupus mice by using anticlass-II antibody in vitro. The in vitro culture of B cells from old NZB mice markedly produced Ig without any stimulation, while B cells from NZW mice did not. The addition of anticlass-II antibody (anti-Iad antibody) to the culture...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1991.tb01607.x
更新日期:1991-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2001.tb02628.x
更新日期:2001-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2004.tb03614.x
更新日期:2004-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1981.tb00049.x
更新日期:1981-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03758.x
更新日期:2005-01-01 00:00:00
abstract::Staphylococcus aureus strains isolated from bovine intramammary infection (mastitis) were tested for adhesion to bovine mammary epithelial cells after growth in milk whey or TSB. Bacteria grown in milk whey adhered more efficiently to mammary gland epithelial cells in vitro than the corresponding homologous bacteria g...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01781.x
更新日期:1994-01-01 00:00:00
abstract::Thrombin-activatable fibrinolysis inhibitor (TAFI) is an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). TAFI in plasma is presumed to influence vascular disease in its role as a fibrinol...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb03939.x
更新日期:2007-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2008.00071.x
更新日期:2008-11-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb03979.x
更新日期:2007-01-01 00:00:00
abstract::The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03455.x
更新日期:2003-01-01 00:00:00
abstract::Magnaporthe oryzae chrysovirus 1 strain A (MoCV1-A) is the causal agent of growth repression and attenuated virulence (hypovirulence) of the rice blast fungus, M. oryzae. We have previously reported that heterologous expression of MoCV1-A ORF4 in Saccharomyces cerevisiae results in growth defects, a large central vacu...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
abstract::The test for susceptibility to kanamycin is useful for differentiating between the genera Rhodococcus and Nocardia. All rhodococci except R. equi, R. erythropolis, and R. aurantiacus are susceptible to kanamycin, whereas all nocardiae except N. otitidis-caviarum are resistant to kanamycin. Tests for susceptibility to ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
abstract::Three strains TKU9, TKU49 and TKU50(T) , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infa...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12290
更新日期:2015-09-01 00:00:00
abstract::A chimeric PCV1-2 clone containing the PCV2 capsid gene cloned into the backbone of the nonpathogenic PCV1 genome was recently generated based on PCV2 and PCV1 strains isolated in China. The efficacy of this available candidate inactivated vaccine was evaluated by subjecting conventional pigs to intramuscular immuniza...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
abstract::Burkholderia cepacia complex (Bcc) are opportunistic pathogens implicated with nosocomial infections, and high rates of morbidity and mortality, especially in individuals with cystic fibrosis (CF). B. cepacia are naturally resistant to different classes of antibiotics, and can subvert the host innate immune responses ...
journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/1348-0421.12762
更新日期:2020-02-01 00:00:00
abstract::A 4-year (1996-2000) survey of rotavirus infection involving 2,218 diarrheal fecal specimens of children collected from five regions of Japan was conducted. A total of 642 (28.9%) specimens were found to be rotavirus positive. A changed prevalence pattern of rotavirus G serotype was found with an increase of G9 and G2...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03422.x
更新日期:2003-01-01 00:00:00
abstract::A sensitive, quantitative, short-time, and reproducible focus assay for Japanese encephalitis (JE) virus is described. After 2 or 3 days of incubation, the infected cells were treated with anti-JE virus serum and complement, and subsequently stained with trypan blue; then clear foci were produced. This method made it ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00802.x
更新日期:1985-01-01 00:00:00
abstract::A monoclonal antibody (MoAb) to human neutrophils, designated 3H9, was established by screening for the inhibition of neutrophil adherence to plastic plates containing a medium supplemented with fetal calf serum (FCS medium). The antigen recognized by 3H9 was shown to be present on human leukocytes and found at the hi...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01174.x
更新日期:1997-01-01 00:00:00
abstract::Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2002.tb02759.x
更新日期:2002-01-01 00:00:00