Surface characteristics of gram-negative and gram-positive bacteria in an atomic force microscope image.

Abstract:

:Bacterial images can be obtained rather easily with an atomic-force microscope (AFM) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. The bacterial shapes or the presence of flagella can be clearly recognized in these magnification ranges. In addition, we were also able to distinguish between gram-negative and gram-positive bacteria based on the specific wavy surface appearance of the former. AFM could thus be a useful tool for the identification of bacteria in the resolution range between electron and light microscopy.

journal_name

Microbiol Immunol

authors

Umeda A,Saito M,Amako K

doi

10.1111/j.1348-0421.1998.tb02266.x

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

159-64

issue

3

eissn

0385-5600

issn

1348-0421

journal_volume

42

pub_type

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