Integration of bovine leukemia virus DNA in the genomes of bovine lymphosarcoma cells.

Abstract:

:Integration of bovine leukemia virus (BLV) in the genomes of infected cells was investigated in cattle with enzootic bovine leukosis (EBL) and sporadic bovine leukosis (SBL). Southern blot hybridization of BLV cDNA to Eco RI and Xba I restriction fragments of EBL tumor DNAs revealed that: 1) one to four or more copies of proviral DNA were integrated per genome; 2) the restriction pattern of the integrated proviral DNA was the same in two or three different tumors from the same animals; and 3) different patterns were observed among tumors from four different animals. These findings suggest the monoclonal origin of different tumors in an individual animal and the existence of multiple chromosomal integration sites of BLV provirus. DNAs from several SBL tumors were also analyzed with the same restriction enzymes, but with both representative and cDNA3'-enriched's of BLV RNA. No hybridization bands reactive with representative BLV cDNA could be detected, while several bands appeared to hybridize with cDNA3'-enriched.

journal_name

Microbiol Immunol

authors

Onuma M,Sagata N,Okada K,Ogawa Y,Ikawa Y,Oshima K

doi

10.1111/j.1348-0421.1982.tb00227.x

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

813-20

issue

9

eissn

0385-5600

issn

1348-0421

journal_volume

26

pub_type

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