Construction of a slime negative transposon mutant in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917.

abstract：:Macrophages are extremely heterogeneous mononuclear phagocytes widely distributed throughout the body. They play unique roles in each organ where they reside. Among macrophage subsets, red pulp macrophages (RPMs) that localize in the splenic red pulp, are critical for maintenance of blood homeostasis by actively phago...

journal_title：Microbiology and immunology

authors： Kurotaki D,Uede T,Tamura T

更新日期：2015-02-01 00:00:00

abstract：:Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

journal_title：Microbiology and immunology

authors： Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

更新日期：1993-01-01 00:00:00

abstract：:We have previously identified a heterodimer molecule, Z-1, on guinea pig peritoneal macrophages (M phi s) by the newly prepared monoclonal antibody, anti-Z-1, and Z-1 has been assumed to be the complement receptor type three (CR3) in this species. To clarify this assumption, the cell type distribution of the antigen i...

journal_title：Microbiology and immunology

authors： Tamoto K,Nochi H,Tada M,Kakinuma T,Mori Y

更新日期：1993-01-01 00:00:00

abstract：:Congenitally athymic BALB/cA nu/nu mice were employed to elucidate the role of the thymus in experimental Mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. Chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. Macroscopic...

journal_title：Microbiology and immunology

authors： Tanaka H

更新日期：1979-01-01 00:00:00

abstract：:Attempts were made to study the pathogenicity of some strains of Bacteroides fragilis group in the rat intra-abdominal abscess model. Multiple intraabdominal abscesses were produced in 50 to 70% of animals when an inoculum containing 10(9) CFU/ml of any of the five species of Bacteroides fragilis group was injected. R...

journal_title：Microbiology and immunology

authors： Jotwani R,Gupta U,Watanabe K,Ueno K

更新日期：1992-01-01 00:00:00

abstract：:Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to...

journal_title：Microbiology and immunology

authors： Saruta K,Hoshina S,Machida K

更新日期：1995-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) an arthropod-borne Alphavirus is responsible for chikungunya fever and joint pain etc. Arthralgia is a major symptom; some patients recover early while others suffer for a very long time. Epidemiology and molecular characterization of three whole-genome sequences of CHIKV have been carried ou...

journal_title：Microbiology and immunology

authors： Khan N,Bhat R,Jain V,Raghuvendara B S,Patel AK,Ray P

更新日期：2020-12-21 00:00:00

abstract：:An emetic toxin cereulide, produced by Bacillus cereus, causes emetic food poisonings, but a method for quantitative measurement of cereulide has not been well established. A current detection method is a bioassay method using the HEp-2 cell vacuolation test, but it was unable to measure an accurate concentration. We ...

journal_title：Microbiology and immunology

authors： Kawamura-Sato K,Hirama Y,Agata N,Ito H,Torii K,Takeno A,Hasegawa T,Shimomura Y,Ohta M

更新日期：2005-01-01 00:00:00

abstract：:In a previous study, it was found that polyoxotungstates such as undecatungstosilicate (SiW11) greatly sensitized strains of methicillin-resistant Staphylococcus aureus (MRSA) to beta-lactams. In this report, the effects of SiW11 on several MRSA strains with unique resistant mechanisms were studied. SiW11 was still ef...

journal_title：Microbiology and immunology

authors： Tajima Y

更新日期：2003-01-01 00:00:00

abstract：:Temperature dependence of transfer was examined with ten R plasmids originating from clinical isolates of Salmonella. Six of the plasmids were thermosensitive upon transfer, five of which were originally harbored in S. typhimurium and the remaining one in S. derby. One of these plasmids, pNR502, which conferred resist...

journal_title：Microbiology and immunology

authors： Yoshida Y,Terawaki Y,Nakaya R

更新日期：1978-01-01 00:00:00

abstract：:Interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and other hematopoietic cells. To elucidate IL-5-mediated intracellular mechanisms, we have established IL-5-dependent and -independent murine early B cell lines, J6 and MJ88-1, respect...

journal_title：Microbiology and immunology

authors： Migita M,Yamaguchi N,Katoh S,Mita S,Matsumoto R,Sonoda E,Tsuchiya H,Matsuda I,Tominaga A,Takatsu K

更新日期：1990-01-01 00:00:00

abstract：:Zymographic analysis was performed to know the bacteriolytic enzyme profiles of 4% SDS extracts of oral streptococci, Streptococcus mutans, S. sobrinus, S. sanguis, S. mitis and S. salivarius. We investigated the five strains in each species and found that the profile was very similar among strains of the same species...

journal_title：Microbiology and immunology

authors： Yoshimura G,Komatsuzawa H,Kajimura J,Fujiwara T,Ohara M,Kozai K,Sugai M

更新日期：2004-01-01 00:00:00

abstract：:In the present study, 12 patients with fever of undetermined origin, anemia and icterus were diagnosed with hemoplasma infection by light microscopy, transmission electron microscopy examination and PCR assay after being excluded from other usual febrile diseases. Complement receptor type I (CR1, CD35) expression on t...

journal_title：Microbiology and immunology

authors： Congbin Y,Aibin L,Congli Y,Juan X,Lanjun B,Weiping Z,Zhibiao Y,Xiuguo H

更新日期：2010-08-01 00:00:00

abstract：:Butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii was cultured with peripheral blood mononuclear cells (PBM) in the presence of sub- and/or supra-optimal mitogenic concentrations of concanavalin A (Con A). The addition of Bu-WSA resulted in increased tritiated thymidine incorpora...

journal_title：Microbiology and immunology

authors： Nitta T,Okumura S,Tsushi M,Nakano M

更新日期：1982-07-01 00:00:00

abstract：:Dialyzable factors (DF) were prepared from ribosomal fractions of several organisms including rough mutants of Salmonella typhimurium LT2, salmonella species of different serogroups, other enteric bacteria and gram-positive organisms, and tested for their immunogenicity against S. typhimurium infection in mice. All of...

journal_title：Microbiology and immunology

authors： Kita E,Yasui K,Yasui K,Matsuda Y,Matsuda K,Kashiba S

更新日期：1984-01-01 00:00:00

abstract：:We elucidated the contribution of Valpha14 NKT cells to Th1 response and host resistance against mycobacterial infection. In Valpha14 NKT cell-deficient mice, host defense and DTH response to Mycobacterium bovis BCG were not different from wild-type mice after pulmonary infection. There was no significant difference i...

journal_title：Microbiology and immunology

authors： Kawakami K,Kinjo Y,Uezu K,Yara S,Miyagi K,Koguchi Y,Nakayama T,Taniguchi M,Saito A

更新日期：2002-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...

journal_title：Microbiology and immunology

authors： Otsuka N,Yoshino S,Kawano K,Toyoizumi-Ajisaka H,Shibayama K,Kamachi K

更新日期：2012-07-01 00:00:00

abstract：:Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...

journal_title：Microbiology and immunology

authors： Machida K,Sakurai S,Kondo I,Ikawa S

更新日期：1988-01-01 00:00:00

abstract：:proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

journal_title：Microbiology and immunology

authors： Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5A (NS5A) is a multifunctional protein that is involved in the HCV life cycle and pathogenesis. In this study, a host protein(s) interacting with NS5A by tandem affinity purification were searched for with the aim of elucidating the role of NS5A. An NS5A-interacting prote...

journal_title：Microbiology and immunology

authors： Chen M,Gan X,Yoshino K,Kitakawa M,Shoji I,Deng L,Hotta H

更新日期：2016-06-01 00:00:00

abstract：:Alpha-fetoprotein (AFP) expression is observed in embryonic tissues and, the expression of this protein is absent in normal adult tissues. The re-elevation of serum AFP strongly suggests generation of a malignant tumor in an adult. We demonstrated here that AFP-producing gastric cancer (AFP-gastric cancer) could be tr...

journal_title：Microbiology and immunology

authors： Miura Y,Kataoka H,Joh T,Tada T,Asai K,Nakanishi M,Okada N,Okada H

更新日期：2004-01-01 00:00:00

abstract：:Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

journal_title：Microbiology and immunology

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2001-01-01 00:00:00

abstract：:Cauliflower mosaic virus (CaMV) open reading frame (ORF) VI product (P6) has been shown to be the major constituent of viral inclusion body, to function as a post-transcriptional transactivator, and to be essential for infectivity on whole plants. Although these findings suggest that P6 has an important role in viral ...

journal_title：Microbiology and immunology

authors： Kobayashi K,Tsuge S,Nakayashiki H,Mise K,Furusawa I

更新日期：1998-01-01 00:00:00

abstract：:L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in bo...

journal_title：Microbiology and immunology

authors： Nishikawa F,Kita E,Yamada H,Nakano A,Kashiba S

更新日期：1994-01-01 00:00:00

abstract：:In order to analyze the host-parasite interactions in experimental trichomoniasis, the growth of Trichomonas foetus in the peritoneal cavity and changes in the peritoneal exudate cells were followed in mice treated with dextran sulfate 500 (DS 500), a known macrophage-toxic agent. Light microscopic observation showed ...

journal_title：Microbiology and immunology

authors： Oka Y,Hayashi H,Ishikawa F,Osaki H

更新日期：1980-01-01 00:00:00

abstract：:Susceptibility of eight strains of influenza A and B viruses to interferon and to poly(I) . poly(C) were determined by the plaque reduction method. All strains tested were slightly less susceptible than vesicular stomatitis virus (VSV) in an established line of canine kidney (MDCK) cells. The 50% plaque depression dos...

journal_title：Microbiology and immunology

authors： Machida H,Kuninaka A,Yoshino H

更新日期：1980-01-01 00:00:00

abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:Unmethylated CpG-ODN are known to enhance Th1-type immune response. However, optimal sequences of CpG-ODN for activating Th1-type immune cells vary among species. It is necessary to identify the effective CpG-ODN sequences in each species. In the present study, in order to identify the sequences of CpG-ODN that produc...

journal_title：Microbiology and immunology

authors： Satoh R,Kotake M,Takano T,Motokawa K,Gemma T,Watanabe R,Arai S,Hohdatsu T

更新日期：2011-03-01 00:00:00

abstract：:We attempted to detect Coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (PCR) method. Ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near Sapporo, Hokkaido. C. burnetii was detected in...

journal_title：Microbiology and immunology

authors： Yanase T,Muramatsu Y,Inouye I,Okabayashi T,Ueno H,Morita C

更新日期：1998-01-01 00:00:00

abstract：:Virulence of Coxsackie virus B3 (CB3) was compared in mice between strain SK-74 isolated from a patient and strain T-70 isolated from a healthy child as well as between prototype strain Nancy and its mouse-passaged derivative strain PMH. Strain SK-74 showed a high mortality (40-80%), while strain T-70 did not induce d...

journal_title：Microbiology and immunology

authors： Komatsu T,Hashimoto I,Kohara T

更新日期：1983-01-01 00:00:00