Protective capacity of L-form Salmonella typhimurium against murine typhoid in C3H/HeJ mice.

Abstract:

:L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in both the liver and spleen with the maximal growth 2-3 weeks after immunization and thereafter it persisted in the liver until 24 weeks. Protective immunity began to work between 4 and 6 weeks after immunization, and it remained active as long as the L forms colonized the liver (until 24 weeks after immunization). Vaccination with the L form induced a population of T cells responding to L-form whole-cell lysate (WCL), while delayed-type hypersensitivity (DTH) to the extract of S. typhimurium was induced after the establishment of solid immunity. Moreover, neither T-cell responses nor DTH to heat-killed S. typhimurium was generated. In addition, antibody responses were elicited to WCL but not to heat-killed S. typhimurium. These results indicate that protection conferred by the L forms is attributable to the persistent colonization of the L forms rather than the presence of DTH, and also that Salmonella cytoplasmic antigens are involved in induction of immunological responses by vaccination with the L forms.

journal_name

Microbiol Immunol

authors

Nishikawa F,Kita E,Yamada H,Nakano A,Kashiba S

doi

10.1111/j.1348-0421.1994.tb01754.x

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

129-37

issue

2

eissn

0385-5600

issn

1348-0421

journal_volume

38

pub_type

杂志文章
  • Genetic identification of Staphylococcus aureus by polymerase chain reaction using single-base-pair mismatch in 16S ribosomal RNA gene.

    abstract::Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1995.tb03280.x

    authors: Saruta K,Hoshina S,Machida K

    更新日期:1995-01-01 00:00:00

  • An assay for the receptor-destroying activity of influenza C virus.

    abstract::We have developed a convenient method for assaying the receptor-destroying enzyme (RDE) activity of influenza C virus. This method measures the ability of the RDE to destroy the hemagglutination-inhibition activity of a potent inhibitor present in rat serum. Some physico-chemical properties of the RDE of influenza C v...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1985.tb00910.x

    authors: Sugawara K,Kitame F,Homma M,Nakamura K

    更新日期:1985-01-01 00:00:00

  • Process of consecutive cell divisions and separations in a regular tetrads-forming mutant of Micrococcus lysodeikticus (luteus).

    abstract::A mutant MT of Micrococcus lysodeikticus (luteus) IFO 3333, whose minimum growing unit is not a single cell, but a tetrad unlike the wild-type divides by binary fission of each monococcus, and then separates first into two daughter tetrads, second into four tetrads and third into eight tetrads. The three planes of eit...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1978.tb00391.x

    authors: Monodane T,Matsushima Y,Kotani S

    更新日期:1978-01-01 00:00:00

  • Application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation.

    abstract::Water-insoluble glucan (WIG) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. Glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze WIG. The pu...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12214

    authors: Otsuka R,Imai S,Murata T,Nomura Y,Okamoto M,Tsumori H,Kakuta E,Hanada N,Momoi Y

    更新日期:2015-01-01 00:00:00

  • Blockade of Salmonella enteritidis passage across the basolateral barriers of human intestinal epithelial cells by specific antibody.

    abstract::Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb02522.x

    authors: Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

    更新日期:2000-01-01 00:00:00

  • Effect of the extract made from Cochinchina momordica seeds on the humoral immune responses of mice to a commercial foot-and-mouth disease vaccine (serotypes O and Asia 1).

    abstract::The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosupp...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2009.00180.x

    authors: Sakwiwatkul K,Li RL,Song XM,Hu SH

    更新日期:2010-01-01 00:00:00

  • Characterization of an outer membrane protein gene, pgmA, and its gene product from Porphyromonas gingivalis.

    abstract::A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1999.tb03354.x

    authors: Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

    更新日期:1999-01-01 00:00:00

  • Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts.

    abstract::Cauliflower mosaic virus (CaMV) open reading frame (ORF) VI product (P6) has been shown to be the major constituent of viral inclusion body, to function as a post-transcriptional transactivator, and to be essential for infectivity on whole plants. Although these findings suggest that P6 has an important role in viral ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1998.tb02298.x

    authors: Kobayashi K,Tsuge S,Nakayashiki H,Mise K,Furusawa I

    更新日期:1998-01-01 00:00:00

  • In vitro models for analysis of the hepatitis C virus life cycle.

    abstract::Chronic hepatitis C virus (HCV) infection affects approximately 170 million people worldwide. HCV infection is a major global health problem as it can be complicated with liver cirrhosis and hepatocellular carcinoma. So far, there is no vaccine available and the non-specific, interferon (IFN)-based treatments now in u...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1348-0421.2011.00403.x

    authors: Aly HH,Shimotohno K,Hijikata M,Seya T

    更新日期:2012-01-01 00:00:00

  • Electron microscopic study of hemadsorption on vaccinia virus infected cells.

    abstract::Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1977.tb00327.x

    authors: Saburi Y,Okuda K,Takahashi T,Tadokoro I

    更新日期:1977-10-20 00:00:00

  • Evaluation of antiherpetic compounds using a gastric cancer cell line: pronounced activity of BVDU against herpes simplex virus replication.

    abstract::We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV-induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric as...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb01080.x

    authors: Kodama E,Igarashi A,Mori S,Hashimoto K,Suzuki T,De Clercq E,Shigeta S

    更新日期:1996-01-01 00:00:00

  • Japanese patients with chronic fatigue syndrome are negative for known retrovirus infections.

    abstract::Although chronic fatigue syndrome (CFS) is known to be the syndrome that begins with an acute flu-like illness that may be due to the exposure to an infectious agent, there has been no convincing evidence on the causative agents. Recently, human T-lymphotropic virus type II (HTLV-II)-like virus has been reported to be...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb01705.x

    authors: Honda M,Kitamura K,Nakasone T,Fukushima Y,Matsuda S,Nishioka K,Matsuda J,Hashimoto N,Yamazaki S

    更新日期:1993-01-01 00:00:00

  • Intestinal microflora and bile acids. Effect of bile acids on the distribution of microflora and bile acid in the digestive tract of the rat.

    abstract::The population levels of intestinal microflora and bile acid composition in the digestive tract were examined in rats fed bile acids to determine the relationships between gastrointestinal microflora and the host. The population level of Bacteroides was increased in the ceca of rats fed cholic acid or deoxycholic acid...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1980.tb00578.x

    authors: Sakai K,Makino T,Kawai Y,Mutai M

    更新日期:1980-01-01 00:00:00

  • Antichlamydial activity of ofloxacin.

    abstract::The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1986.tb02970.x

    authors: Hirai K,Une T

    更新日期:1986-01-01 00:00:00

  • Comparison of virulence between Seoul virus strain SR-11 and Hantaan virus strain 76-118 of hantaviruses in newborn mice.

    abstract::Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb01677.x

    authors: Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

    更新日期:1993-01-01 00:00:00

  • Heterologous expression of a gene of Magnaporthe oryzae chrysovirus 1 strain A disrupts growth of the human pathogenic fungus Cryptococcus neoformans.

    abstract::Magnaporthe oryzae chrysovirus 1 strain A (MoCV1-A) is the causal agent of growth repression and attenuated virulence (hypovirulence) of the rice blast fungus, M. oryzae. We have previously reported that heterologous expression of MoCV1-A ORF4 in Saccharomyces cerevisiae results in growth defects, a large central vacu...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12148

    authors: Urayama S,Fukuhara T,Moriyama H,Toh-E A,Kawamoto S

    更新日期:2014-05-01 00:00:00

  • Reactivation of herpes simplex virus type 1 in cells of latently infected mouse trigeminal ganglia cultured in serum-free medium.

    abstract::Herpes simplex virus type 1 (HSV-1) was reactivated more rapidly in cells of latently infected mouse trigeminal ganglia which were cultured in serum-free medium (after 3.7 days of cultivation) than in those cultured in serum-containing Dulbecco's modified Eagle's medium (after 8.5 days of cultivation). The concentrati...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb03332.x

    authors: Kimura S

    更新日期:1996-01-01 00:00:00

  • Establishment of an assay system for the detection of translated materials of T-cell-replacing factor mRNA in Xenopus oocytes.

    abstract::We established an assay system for detecting T cell-replacing factor (TRF) activity of translated materials in Xenopus oocytes of poly (A)-positive mRNA extracted from a T cell hybrid cell line, B151K12 (B151) which constitutively produces TRF. Since it was difficult to detect TRF activity of the translated products o...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1986.tb03005.x

    authors: Tominaga A,Matsumoto M,Takahashi T,Harada N,Takatsu K

    更新日期:1986-01-01 00:00:00

  • Structural requirements of cholesterol for binding to Vibrio cholerae hemolysin.

    abstract::Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2006.tb03848.x

    authors: Ikigai H,Otsuru H,Yamamoto K,Shimamura T

    更新日期:2006-01-01 00:00:00

  • RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux.

    abstract::RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susce...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01913.x

    authors: Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

    更新日期:1997-01-01 00:00:00

  • Analysis of the phospholipase C gene of Clostridium perfringens KZ1340 isolated from Antarctic soil.

    abstract::Clostridium perfringens KZ1340 isolated from Antarctic soil was first classified as Clostridium plagarum and later as a lecithinase-negative variant of C. perfringens. Although the strain produced no detectable lecithinase (phospholipase C, PLC) activity in the culture supernatant, it was shown by Southern blot hybrid...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb03344.x

    authors: Kameyama K,Matsushita O,Katayama S,Minami J,Maeda M,Nakamura S,Okabe A

    更新日期:1996-01-01 00:00:00

  • Detection and molecular characterization of adenoviruses in Korean children hospitalized with acute gastroenteritis.

    abstract::Human adenoviruses (HAdVs) are an important cause of acute gastroenteritis in children. However, few studies on the epidemiology or types of HAdVs associated with acute gastroenteritis have been conducted in Korea. Therefore, in the present study, the incidence of HAdV in 2064 stool samples from Korean children hospit...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2012.00469.x

    authors: Lee JI,Lee GC,Chung JY,Han TH,Lee YK,Kim MS,Lee CH

    更新日期:2012-08-01 00:00:00

  • Accumulation of eosinophils and monocytes in lymphoid organs of chick-embryos. II. Effect of mitogenic stimulation.

    abstract::The mitogen effect on migration of eosinophils and monocytes was studied in embryonic chickens. On the 13th embryonic day, chickens were injected with mitogens, such as concanavalin A (Con A), phytohemagglutinin-P (PHA-P), and lipopolysaccharide (LPS), into the allantoic cavity, and the mitogenic effect was estimated ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1979.tb00491.x

    authors: Moriya O,Ichikawa Y

    更新日期:1979-01-01 00:00:00

  • Nucleotide sequence of the replication region of plasmid R401 and its incompatibility function.

    abstract::The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences fla...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1985.tb00839.x

    authors: Tabuchi A

    更新日期:1985-01-01 00:00:00

  • Metastasis-promoting activity of a novel molecule, Ag 243-5, derived from mycoplasma, and the complete nucleotide sequence.

    abstract::We found that mycoplasma-infected cells have a higher ability to metastasize in vivo than non-mycoplasma-infected cells. To investigate this phenomenon, we obtained a monoclonal antibody, MAb 243-5, by immunization with Mycoplasma arginini-infected RPMI 4788 cells. This MAb recognized a mycoplasmal protein with an MW ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1995.tb02218.x

    authors: Ushio S,Iwaki K,Taniai M,Ohta T,Fukuda S,Sugimura K,Kurimoto M

    更新日期:1995-01-01 00:00:00

  • Requirement of carbohydrates for cell fusion induced by vesicular stomatitis virus.

    abstract::The fusion of BHK-21-KB cells by vesicular stomatitis virus was not induced in Eagle's minimal essential medium without glucose. In medium containing glucose, the rate of polykaryocyte formation decreased as the concentration of glucose was reduced below 5 mM. However, no reduction in virus production 24 hr after infe...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1981.tb00039.x

    authors: Takehara M

    更新日期:1981-01-01 00:00:00

  • Effect of constitutively expressed phoP gene on the localization of Salmonella typhimurium within Mac-1 positive phagocytes.

    abstract::Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb01261.x

    authors: Matsui H,Eguchi M,Kikuchi Y

    更新日期:2001-01-01 00:00:00

  • The presence of antibodies against the AD2 epitope of cytomegalovirus glycoprotein B is associated with acute rejection after renal transplantation.

    abstract::The aim of this study was to evaluate the association between antibodies against cytomegalovirus (CMV) glycoprotein B (gB) and acute rejection after transplantation. Seventy-seven consecutive renal transplant recipients in a D + /R+ setting were studied. Biopsy-proven rejection occurred in 35% of the recipients. Among...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12112

    authors: Ishibashi K,Tokumoto T,Shirakawa H,Oguro T,Yanagida T,Takahashi N,Nomiya M,Haga N,Aikawa K,Tanabe K,Inoue N,Kojima Y,Suzutani T

    更新日期:2014-01-01 00:00:00

  • NK1.1(+) cells regulate neutrophil migration in mice with Acinetobacter baumannii pneumonia.

    abstract::Acinetobacter baumannii is a major cause of both community-associated and nosocomial infections worldwide. These infections are difficult to treat because the bacterium rapidly develops resistance to multiple antibiotics. However, little is known about the nature of the innate cellular response to A. baumannii infecti...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2011.00402.x

    authors: Tsuchiya T,Nakao N,Yamamoto S,Hirai Y,Miyamoto K,Tsujibo H

    更新日期:2012-02-01 00:00:00

  • Enhanced effect of BCG vaccine against pulmonary Mycobacterium tuberculosis infection in mice with lung Th17 response to mycobacterial heparin-binding hemagglutinin adhesin antigen.

    abstract::Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12340

    authors: Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

    更新日期:2015-12-01 00:00:00