Abstract:
:A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer membrane protein A gene. The gene product, PgmA, was sensitive to protease, and was detected as a 60-kDa protein from wild-type strains with trichloroacetic acid treatment, which was carried out to destroy intrinsic proteases, and from protease-deficient mutants without this treatment prior to electrophoresis. PgmA was indeed present in the membrane fraction. Its nature was determined to be that of outer membrane proteins in gram-negative bacteria based on attempts at differential extraction of inner membrane proteins with detergents. No evidence has been found thus far from functional analyses that this protein is related to fimbrial morphogenesis and functions or to serum resistance of this organism.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura Fdoi
10.1111/j.1348-0421.1999.tb03354.xkeywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
937-46issue
10eissn
0385-5600issn
1348-0421journal_volume
43pub_type
杂志文章abstract::Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively...
journal_title:Microbiology and immunology
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abstract::The aim of this study was to evaluate the association between antibodies against cytomegalovirus (CMV) glycoprotein B (gB) and acute rejection after transplantation. Seventy-seven consecutive renal transplant recipients in a D + /R+ setting were studied. Biopsy-proven rejection occurred in 35% of the recipients. Among...
journal_title:Microbiology and immunology
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doi:10.1111/1348-0421.12112
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00900.x
更新日期:1985-01-01 00:00:00
abstract::Using a new plaque cloning technique, we obtained unique Chlamydia trachomatis strains which were confirmed to be free of the 7.5-kb common plasmid and glycogen in inclusions. The in vitro susceptibility of these strains to various chemotherapeutic agents was tested by comparison with their parent strains and clinical...
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abstract::Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet-activator-factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS als...
journal_title:Microbiology and immunology
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更新日期:1982-01-01 00:00:00
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更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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更新日期:1981-01-01 00:00:00
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更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
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更新日期:1997-01-01 00:00:00
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journal_title:Microbiology and immunology
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更新日期:1988-01-01 00:00:00
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journal_title:Microbiology and immunology
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更新日期:2001-01-01 00:00:00
abstract::Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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更新日期:1978-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Microbiology and immunology
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更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
abstract::The population levels of intestinal microflora and bile acid composition in the digestive tract were examined in rats fed bile acids to determine the relationships between gastrointestinal microflora and the host. The population level of Bacteroides was increased in the ceca of rats fed cholic acid or deoxycholic acid...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1980.tb00578.x
更新日期:1980-01-01 00:00:00