The usefulness of semi-solid medium in the isolation of highly virulent Leptospira strains from wild rats in an urban area of Fukuoka, Japan.

abstract：:The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...

journal_title：Microbiology and immunology

authors： Ohwada K,Kitame F,Sugawara K,Nishimura H,Homma M,Nakamura K

更新日期：1987-01-01 00:00:00

abstract：:Seroprevalence of Bartonella henselae was investigated in Japan in 48 individuals clinically suspected of having cat-scratch disease (CSD), 159 patients with cardiovascular diseases, and 129 healthy veterinary students. Of 48 CSD-suspected patients examined, 19 (39.6%) were positive for B. henselae-IgG and 4 (8.3%) fo...

journal_title：Microbiology and immunology

authors： Kikuchi E,Maruyama S,Sakai T,Tanaka S,Yamaguchi F,Hagiwara T,Katsube Y,Mikami T

更新日期：2002-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights wer...

journal_title：Microbiology and immunology

authors： Konishi E,Hotta S

更新日期：1980-01-01 00:00:00

abstract：:Influenza A virus (IAV) PA-X is a critical ribonuclease protein involved in host cell shutoff but its role in modulating the host immune response to IAV infection remains to be addressed. In this study, host cellular proteins that directly interact with PA-X were screened to investigate the biological function of PA-X...

journal_title：Microbiology and immunology

authors： Li M,Qi W,Chang Q,Chen R,Zhen D,Liao M,Wen J,Deng Y

更新日期：2020-11-26 00:00:00

abstract：:Interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and other hematopoietic cells. To elucidate IL-5-mediated intracellular mechanisms, we have established IL-5-dependent and -independent murine early B cell lines, J6 and MJ88-1, respect...

journal_title：Microbiology and immunology

authors： Migita M,Yamaguchi N,Katoh S,Mita S,Matsumoto R,Sonoda E,Tsuchiya H,Matsuda I,Tominaga A,Takatsu K

更新日期：1990-01-01 00:00:00

abstract：:Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinicall...

journal_title：Microbiology and immunology

authors： Shimada K,Hayashi HK,Ookubo Y,Iwamaru Y,Imamura M,Takata M,Schmerr MJ,Shinagawa M,Yokoyama T

更新日期：2005-01-01 00:00:00

abstract：:An emetic toxin cereulide, produced by Bacillus cereus, causes emetic food poisonings, but a method for quantitative measurement of cereulide has not been well established. A current detection method is a bioassay method using the HEp-2 cell vacuolation test, but it was unable to measure an accurate concentration. We ...

journal_title：Microbiology and immunology

authors： Kawamura-Sato K,Hirama Y,Agata N,Ito H,Torii K,Takeno A,Hasegawa T,Shimomura Y,Ohta M

更新日期：2005-01-01 00:00:00

abstract：:We prepared polyclonal antibody specific to Mycoplasma pneumoniae. Using this antibody, we developed a latex agglutination test (LAT) for detecting the organism in respiratory exudates as rapid diagnosis of M. pneumoniae infection. Further, LAT was compared with DNA-probe test (DP) which was the only commercially avai...

journal_title：Microbiology and immunology

authors： Masayoshi T,Hirai Y,Kanemasa Y

更新日期：1992-01-01 00:00:00

abstract：:Streptococcus anginosus appears to be able to adhere to cultured epithelial cells or fibronectin and this may be associated with bacterial pathogenicity. In the present study, the molecular characteristics and virulence of the fibronectin-binding protein (FBP), Fbp62, of S. anginosus were investigated in animal models...

journal_title：Microbiology and immunology

authors： Kodama Y,Ishikawa T,Shimoyama Y,Sasaki D,Kimura S,Sasaki M

更新日期：2018-10-01 00:00:00

abstract：:Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of ...

journal_title：Microbiology and immunology

authors： Wang R,Chen S,Jin J,Ren F,Li Y,Qiao Z,Wang Y,Zhao L

更新日期：2015-05-01 00:00:00

abstract：:A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine af...

journal_title：Microbiology and immunology

authors： Guo X,Jia H,Zhang Q,Yuan W,Zhu G,Xin T,Zhu H,Sánchez-Vizcaíno JM

更新日期：2012-05-01 00:00:00

abstract：:Previously we reported that mice infected recurrently with live Fusobacterim nucleatum (Fn) synthesize a significant amount of NO between 12 hr and 24 hr after the Fn injection. We now investigated whether the NO has the capability of killing Fn, a gram-negative rod periodontal pathogen. The mice were divided into thr...

journal_title：Microbiology and immunology

authors： Kato C,Mikami M,Suzuki A,Saito K

更新日期：2003-01-01 00:00:00

abstract：:The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

journal_title：Microbiology and immunology

authors： Hirai K,Une T

更新日期：1986-01-01 00:00:00

abstract：:Clostridium perfringens KZ1340 isolated from Antarctic soil was first classified as Clostridium plagarum and later as a lecithinase-negative variant of C. perfringens. Although the strain produced no detectable lecithinase (phospholipase C, PLC) activity in the culture supernatant, it was shown by Southern blot hybrid...

journal_title：Microbiology and immunology

authors： Kameyama K,Matsushita O,Katayama S,Minami J,Maeda M,Nakamura S,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:Methylcellulose-elicited peritoneal macrophages from BALB/c mice were cultivated in vitro and inoculated with dengue virus (DV). At intervals thereafter portions of the culture fluids were taken and titrated for viral infectivity. Extracts from Ascaris suum and Parascaris equorum, either crude or Sephadex G-100 fracti...

journal_title：Microbiology and immunology

authors： Wiharta AS,Hotta H,Hotta S,Matsumura T,Sujudi,Tsuji M

更新日期：1985-01-01 00:00:00

abstract：:The immunostimulatory effect of intragastrically or parenterally administered beta-(1-->3; 1-->4) glucan, extracted from oats (ObetaG), on disease resistance to Eimeria vermiformis was studied in C57BL/6 mice. Multiple administrations of ObetaG by intragastric or subcutaneous routes reduced fecal oocyst shedding compa...

journal_title：Microbiology and immunology

authors： Yun CH,Estrada A,Van Kessel A,Gajadhar A,Redmond M,Laarveld B

更新日期：1998-01-01 00:00:00

abstract：:Four N-acetylchito-oligosaccharides, from tetra-N-acetylchitotetraose (NACOS-4) to hepta-N-acetylchitoheptaose (NACOS-7), were found to increase the number of peritoneal exudate cells (PEC) in male BALB/c mice after 3 hr intraperitoneal administration of 50 mg/kg of each oligosaccharide. The number of attracted cells,...

journal_title：Microbiology and immunology

authors： Suzuki K,Tokoro A,Okawa Y,Suzuki S,Suzuki M

更新日期：1986-01-01 00:00:00

abstract：:We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for Lyme disease was compared with that of a conventional indirect immunoperoxidase tec...

journal_title：Microbiology and immunology

authors： Fujita H,Sato T,Ohara Y,Homma M

更新日期：1991-01-01 00:00:00

abstract：:The colH gene encoding 116-kDa collagenase of Clostridium histolyticum (cColH) was cloned into an Escherichia coli-Bacillus subtilis shuttle vector to develop a method for purification of recombinant collagenase (rColH). When plasmid pJCM310 containing the colH gene was introduced into B. subtilis DB104 and the transf...

journal_title：Microbiology and immunology

authors： Jung CM,Matsushita O,Katayama S,Minami J,Ohhira I,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian stra...

journal_title：Microbiology and immunology

authors： Yamagata J,Ahmed K,Khawplod P,Mannen K,Xuyen DK,Loi HH,Dung NV,Nishizono A

更新日期：2007-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) an arthropod-borne Alphavirus is responsible for chikungunya fever and joint pain etc. Arthralgia is a major symptom; some patients recover early while others suffer for a very long time. Epidemiology and molecular characterization of three whole-genome sequences of CHIKV have been carried ou...

journal_title：Microbiology and immunology

authors： Khan N,Bhat R,Jain V,Raghuvendara B S,Patel AK,Ray P

更新日期：2020-12-21 00:00:00

abstract：:A human T cell hybridoma clone, F5, producing high levels of macrophage migration inhibitory factor (MIF) was established by the emetine-actinomycin D selection method. This clone produced two species of MIF which were separated on a Phenyl Sepharose column. We purified MIF-2 (the more hydrophobic species of the two) ...

journal_title：Microbiology and immunology

authors： Hirose S,Ooki S,Higuchi M,Osawa T

更新日期：1991-01-01 00:00:00

abstract：:A total of 55 strains of Clostridium sordellii, 21 lethal toxin-positive and 34 lethal toxin-negative, were tested for cytotoxin production in brain heart infusion medium supplemented with 0.2% Na2HPO4 (m-BHI) and cooked-meat-glucose (CMG) medium using baby hamster kidney (BHK-21/WI-2) cells as indicator cells. The m-...

journal_title：Microbiology and immunology

authors： Nakamura S,Tanabe N,Yamakawa K,Nishida S

更新日期：1983-01-01 00:00:00

abstract：:A solubilized sheep red blood cell (SRBC) antigen (supernatant fraction obtained by centrifuging 10(7)-2 x 108 sonicated SRBC at 6 x 10(4) g for 30 min [Sup-SRBC]), whose ability to inhibit anti-SRBC plaque formation was 70% of that of the original sonicated SRBC, was unable to elicit a detectable antibody response in...

journal_title：Microbiology and immunology

authors： Nagase F,Nakashima I,Nagase N,Kato N

更新日期：1982-01-01 00:00:00

abstract：:Vpr, one of the accessory molecules of HIV-1, has been demonstrated to arrest the cell cycle at the G2 phase. This Vpr-mediated cell cycle arrest is implicated to have an important role in the viral life cycle. In the present study, we quantitate the extent of Vpr-mediated cell cycle arrest with the use of a bicistron...

journal_title：Microbiology and immunology

authors： Yamaguchi T,Watanabe N,Nakauchi H,Koito A

更新日期：1999-01-01 00:00:00

abstract：:In order to elucidate the molecular characteristics of Japanese encephalitis (JE) virus in Okinawa, 23 strains of JE virus isolated in a 25-year span were sequenced for the 240 nucleotides of the C-preM junction region and 111 nucleotides of the E gene region and compared with those of reference strains isolated in ma...

journal_title：Microbiology and immunology

authors： Ma SP,Arakaki S,Makino Y,Fukunaga T

更新日期：1996-01-01 00:00:00

abstract：:Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been ...

journal_title：Microbiology and immunology

authors： Hatch WO,Scalarone GM

更新日期：2013-11-01 00:00:00

abstract：:Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, Europe...

journal_title：Microbiology and immunology

authors： Wang J,Masuzawa T,Komikado T,Yanagihara Y

更新日期：1997-01-01 00:00:00

abstract：:An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

journal_title：Microbiology and immunology

authors： Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

更新日期：2013-06-01 00:00:00

abstract：:In Streptococcus pneumoniae, heat shock induces the synthesis of 65-, 73-, and 84-kDa proteins, and ethanol shock induces a 104-kDa protein. In this study, the 65-, 84-, and 104-kDa proteins were identified as members of the GroEL, ClpL and alcohol dehydrogenase families, respectively, and the general properties of th...

journal_title：Microbiology and immunology

authors： Choi IH,Shim JH,Kim SW,Kim SN,Pyo SN,Rhee DK

更新日期：1999-01-01 00:00:00