An indirect microhemagglutination test for Lyme disease.

Abstract:

:We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for Lyme disease was compared with that of a conventional indirect immunoperoxidase technique (IP) using sera from the patients with syphilis, randomly-chosen outpatients unrelated to Lyme disease, and from normal individuals. The IMHA was more specific than IP, giving little cross-reaction with syphilis. The IMHA also gave little background reactions with the sera of individuals unrelated to Lyme disease or syphilis. Therefore, the IMHA could be a useful test for the serodiagnosis of Lyme disease.

journal_name

Microbiol Immunol

authors

Fujita H,Sato T,Ohara Y,Homma M

doi

10.1111/j.1348-0421.1991.tb01590.x

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

589-92

issue

7

eissn

0385-5600

issn

1348-0421

journal_volume

35

pub_type

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