Detection of human cytomegalovirus DNA in breast milk by means of polymerase chain reaction.

Abstract:

:Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of 33 samples collected over 1 month after delivery. Next, we tried to amplify HCMV DNA from the breast milk samples from HCMV seropositive mothers and seronegative mothers at 1 month after delivery by polymerase chain reaction. HCMV DNA was detected in 12 of 13 samples from seropositive mothers and in none of 7 samples from seronegative mothers. It was thought that all women seropositive for HCMV principally shed the virus into their breast milk at 1 month after delivery.

journal_name

Microbiol Immunol

authors

Hotsubo T,Nagata N,Shimada M,Yoshida K,Fujinaga K,Chiba S

doi

10.1111/j.1348-0421.1994.tb01862.x

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

809-11

issue

10

eissn

0385-5600

issn

1348-0421

journal_volume

38

pub_type

杂志文章
  • Further search for small molecular inactivants capable of eliciting respiratory mucosal immunogenicity by modifying Sendai virus core RNA.

    abstract::Five groups of 32 chemicals were examined regarding their immunological functions as modifier inactivants to make inactivated Sendai nasal vaccine using a contact exposure experiment, direct immunofluorescent method, and serum HI titer. (1) Five of the nine reactive groups of reactive dyes (2-chloropyridine, 2, 4, 6-t...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb01138.x

    authors: Miyamae T

    更新日期:1996-01-01 00:00:00

  • Epidemiology and Molecular Characterization of Chikungunya Virus from Human Cases in North India, 2016.

    abstract::Chikungunya virus (CHIKV) an arthropod-borne Alphavirus is responsible for chikungunya fever and joint pain etc. Arthralgia is a major symptom; some patients recover early while others suffer for a very long time. Epidemiology and molecular characterization of three whole-genome sequences of CHIKV have been carried ou...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12869

    authors: Khan N,Bhat R,Jain V,Raghuvendara B S,Patel AK,Ray P

    更新日期:2020-12-21 00:00:00

  • Analysis of cytokine producing activity of intestinal intraepithelial T cells from TCR beta-chain and delta-chain mutant mice.

    abstract::Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utiliz...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01212.x

    authors: Kohyama M,Hachimura S,Nanno M,Ishikawa H,Kaminogawa S

    更新日期:1997-01-01 00:00:00

  • Structural characterization of the O-antigenic polysaccharide chain of Porphyromonas circumdentaria NCTC 12469.

    abstract::Structural studies were carried out on an O-antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of D-glucose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine in a molar ratio of 1:2:1:...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb02622.x

    authors: Matsuo K,Isogai E,Araki Y

    更新日期:2001-01-01 00:00:00

  • Development of a monoclonal antibody against Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) that can detect EBNA-LP expressed in P3HR1 cells.

    abstract::A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody wa...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2005.tb03743.x

    authors: Shaku F,Matsuda G,Furuya R,Kamagata C,Igarashi M,Tanaka M,Kanamori M,Nishiyama Y,Yamamoto N,Kawaguchi Y

    更新日期:2005-01-01 00:00:00

  • Caspase-1 inflammasome activity in patients with Staphylococcus aureus bacteremia.

    abstract::The inflammasome is a multiprotein complex that mediates caspase-1 activation with subsequent maturation of the proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is known to be activated by Staphylococcus aureus, one of the leading causes of bacteremia worldwide. Inflammasome activation and regulation ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12738

    authors: Rasmussen G,Idosa BA,Bäckman A,Monecke S,Strålin K,Särndahl E,Söderquist B

    更新日期:2019-12-01 00:00:00

  • Characterization of a toxin-deficient Clostridium perfringens strain, KZ1340.

    abstract::Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) an...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb03329.x

    authors: Shimizu T,Ohtani K,Ba-Thein W,Inui S,Nakamura S,Hayashi H

    更新日期:1996-01-01 00:00:00

  • Plasma levels of unactivated thrombin activatable fibrinolysis inhibitor (TAFI) are down-regulated in young adult women: analysis of a normal Japanese population.

    abstract::Thrombin-activatable fibrinolysis inhibitor (TAFI) is an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). TAFI in plasma is presumed to influence vascular disease in its role as a fibrinol...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2007.tb03939.x

    authors: Akatsu H,Ishiguro M,Ogawa N,Kanesaka T,Okada N,Yamamoto T,Campbell W,Okada H

    更新日期:2007-01-01 00:00:00

  • A nested PCR assay to detect DNA in sera for the diagnosis of deep-seated trichosporonosis.

    abstract::Deep-seated trichosporonosis caused by Trichosporon asahii has a high mortality rate and a very poor prognosis. New species-specific oligonucleotide primers for T. asahii were developed from a sequence analysis of rRNA genes that included the internal transcribed spacer regions. A nested PCR assay with specific primer...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb01282.x

    authors: Sugita T,Nakajima M,Ikeda R,Niki Y,Matsushima T,Shinoda T

    更新日期:2001-01-01 00:00:00

  • Structural requirements of cholesterol for binding to Vibrio cholerae hemolysin.

    abstract::Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2006.tb03848.x

    authors: Ikigai H,Otsuru H,Yamamoto K,Shimamura T

    更新日期:2006-01-01 00:00:00

  • Detection and nucleotide sequence analysis of human caliciviruses (HuCVs) from samples in non-bacterial gastroenteritis outbreaks in Hokkaido, Japan.

    abstract::Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1999.tb02440.x

    authors: Ohyama T,Yoshizumi S,Sawada H,Uchiyama Y,Katoh Y,Hamaoka N,Utagawa E

    更新日期:1999-01-01 00:00:00

  • Induction of acute arthritis in mice by peptidoglycan derived from gram-positive bacteria and its possible role in cytokine production.

    abstract::The activities of a water-soluble peptidoglycan fragment derived from Staphylococcus epidermidis (SEPS) were examined as to their role in proliferation of spleen mononuclear cells (SMNC) from various strains of mice, the production of cytokines in vitro, and the induction of an inflammatory reaction in vivo. The proli...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb01679.x

    authors: Onta T,Sashida M,Fujii N,Sugawara S,Rikiishi H,Kumagai K

    更新日期:1993-01-01 00:00:00

  • Regulation of marA, soxS, rob, acrAB and micF in Salmonella enterica serovar Typhimurium.

    abstract::Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2008.00075.x

    authors: Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

    更新日期:2008-12-01 00:00:00

  • Effects of mutation in hepatitis C virus nonstructural protein 5A on interferon resistance mediated by inhibition of PKR kinase activity in mammalian cells.

    abstract::The IFN-induced double-stranded RNA (dsRNA)-activated protein kinase PKR is one of the key molecules in the antiviral effects of IFN. To clarify the effects of hepatitis C virus nonstructural protein 5A (NS5A) on antiviral activity of IFN, in particular on PKR kinase activity, in mammalian cells, we established induci...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb01322.x

    authors: Noguchi T,Satoh S,Noshi T,Hatada E,Fukuda R,Kawai A,Ikeda S,Hijikata M,Shimotohno K

    更新日期:2001-01-01 00:00:00

  • Effect of maltose on glucan synthesis by glucosyltransferases of Streptococcus mutans.

    abstract::The effects of added maltose on the activities of a preparation of crude glucosyltransferases (GTases) and purified dextransucrase (DS) were investigated to elucidate the inhibition mechanism of maltose on the synthesis of water-insoluble glucan (ISG) in Streptococcus mutans HS-6. Tri- and tetra-saccharides produced b...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1983.tb00657.x

    authors: Fukui K,Moriyama T

    更新日期:1983-01-01 00:00:00

  • Cell surface of a tetrads-forming mutant of Micrococcus luteus: chemical treatment of the cells and teichuronic acids on the surface.

    abstract::Tetrads-forming mutant MT cells of Micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. The binding of protein A-gold particles to the cel...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1990.tb00992.x

    authors: Monodane T,Tokunaga M,Torii M

    更新日期:1990-01-01 00:00:00

  • An outbreak of neonatal toxic shock syndrome-like exanthematous disease (NTED) caused by methicillin-resistant Staphylococcus aureus (MRSA) in a neonatal intensive care unit.

    abstract::Neonatal toxic shock syndrome-like exanthematous disease (NTED) is a new entity of methicillin-resistant Staphylococcus aureus (MRSA) infection. Most of NTED cases reported previously in the literature were sporadic ones. In the present report, we describe an outbreak of NTED that occurred in a neonatal intensive care...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2002.tb02696.x

    authors: Nakano M,Miyazawa H,Kawano Y,Kawagishi M,Torii K,Hasegawa T,Iinuma Y,Ohta M

    更新日期:2002-01-01 00:00:00

  • RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux.

    abstract::RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susce...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01913.x

    authors: Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

    更新日期:1997-01-01 00:00:00

  • Temperature-sensitive immunoglobulin A-binding and dimerization of C-terminus-impaired protein Arp4 produced in Escherichia coli.

    abstract::A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1992.tb02096.x

    authors: Nakatani T,Higuchi A,Noguchi H

    更新日期:1992-01-01 00:00:00

  • A role for lipid rafts in immune cell signaling.

    abstract::Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1348-0421.2001.tb01259.x

    authors: Katagiri YU,Kiyokawa N,Fujimoto J

    更新日期:2001-01-01 00:00:00

  • Formation of a hexagonal lattice structure by an R-form lipopolysaccharide of Klebsiella: comparative study of preparations extracted by the phenol-water and the phenol-chloroform-petroleum ether methods.

    abstract::An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1985.tb00895.x

    authors: Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

    更新日期:1985-01-01 00:00:00

  • Comparative studies on Borrelia afzelii isolated from a patient of Lyme disease, Ixodes persulcatus ticks, and Apodemus speciosus rodents in Japan.

    abstract::The genospecies Borrelia afzelii was isolated from a patient of Lyme disease in Hokkaido, Japan, for the first time, by culturing the minced erythema lesion in BSK II medium. Two analytical methods, rRNA gene restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) using the specific primer ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1994.tb01801.x

    authors: Nakao M,Miyamoto K,Fukunaga M,Hashimoto Y,Takahashi H

    更新日期:1994-01-01 00:00:00

  • Induction of cross-reactivity in an endogenous viral peptide non-reactive to FBL-3 tumor-specific helper T-cell clones.

    abstract::We previously reported a helper T-cell (Th) epitope (peptide i) which corresponded to the sequence ranging from positions 462 to 479 from the N-terminus of the Friend-murine leukemia virus (F-MuLV) envelope protein (env462-479). Homologous sequences exist in both Moloney-murine leukemia (M-MuLV env452-469) and endogen...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1998.tb02313.x

    authors: Uenishi H,Iwanami N,Yamagishi H,Nakatani T,Kawasaki T,Tamamura H,Fujii N,Kuribayashi K

    更新日期:1998-01-01 00:00:00

  • Susceptibility to killer T cells of gastric cancer cells enhanced by Mitomycin-C involves induction of ATBF1 and activation of p21 (Waf1/Cip1) promoter.

    abstract::Alpha-fetoprotein (AFP) expression is observed in embryonic tissues and, the expression of this protein is absent in normal adult tissues. The re-elevation of serum AFP strongly suggests generation of a malignant tumor in an adult. We demonstrated here that AFP-producing gastric cancer (AFP-gastric cancer) could be tr...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2004.tb03491.x

    authors: Miura Y,Kataoka H,Joh T,Tada T,Asai K,Nakanishi M,Okada N,Okada H

    更新日期:2004-01-01 00:00:00

  • Characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats.

    abstract::The aim of this study was to explore the presence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in a collection of S. pseudintermedius strains isolated from dogs and cats with dermatitis in Japan and to compare their genotypic and phenotypic characteristics. Clonal relationships were determined by pu...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12059

    authors: Bardiau M,Yamazaki K,Ote I,Misawa N,Mainil JG

    更新日期:2013-07-01 00:00:00

  • High molecular weight factor in FCS inhibits Helicobacter pylori VacA-binding to its receptor, RPTPbeta, on AZ-521.

    abstract::VacA, a secretory product of Helicobacter pylori, binds to its cell surface receptor, receptor tyrosine phosphatase (RPTP) beta, leading to cytoplasmic vacuolization of gastric epithelial AZ-521 cells. VacA binding to the cell surface and VacA-dependent vacuolization were inhibited by cell culture media containing fet...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2003.tb02792.x

    authors: Kimura T,Wada A,Nakayama M,Ogushi K,Nishi Y,De Guzman BB,Moss J,Hirayama T

    更新日期:2003-01-01 00:00:00

  • Clinical and bacteriological profiles of patients with typhoid fever treated during 1975-1998 in the Tokyo Metropolitan Komagome Hospital.

    abstract::Patients with typhoid fever presenting to the Tokyo Metropolitan Komagome Hospital during the period 1975-1998 were retrospectively investigated. All cases were diagnosed by a positive culture for Salmonella typhi in either of their clinical specimens. Of the total number of 130 patients, 57% contracted the disease ab...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb02536.x

    authors: Hoshino Y,Masuda G,Negishi M,Ajisawa A,Imamura A,Hachimori K,Takayama N,Yamaguchi T,Kimura M

    更新日期:2000-01-01 00:00:00

  • Neutrophil extracellular Taps play an important role in clearance of Streptococcus suis in vivo.

    abstract::Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12367

    authors: Zhao J,Lin L,Fu L,Han L,Zhang A

    更新日期:2016-04-01 00:00:00

  • Sex- and age-specific prevalences of HBeAg and anti-HBe among HBsAg carriers with or without liver function abnormalities in Okinawa, Japan.

    abstract::We investigated sex- and age-specific prevalences of hepatitis B e antigen (HBeAg) and antibody to HBeAg (anti-HBe) in 1,163 carriers of hepatitis B surface antigen (HBsAg) in Okinawa, Japan, and followed them up for longer than one year in correlation with liver function abnormalities. Elevated serum transaminase lev...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1988.tb01445.x

    authors: Hayashi J,Kashiwagi S,Ikematsu H,Nomura H,Kajiyama W,Noguchi A,Ikeda K

    更新日期:1988-01-01 00:00:00

  • Virological surveillance of human influenza in Indonesia, October 2008-March 2010.

    abstract::Despite the high prevalence of highly pathogenic H5N1 influenza A viruses in Indonesia, epidemiology information on seasonal human influenza is lacking. The present authors, therefore, conducted virologic surveillance in Surabaya, East Java from October 2008 to March 2010. Influenza viruses, including pandemic (H1N1) ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2011.00344.x

    authors: Yamaoka M,Palilingan JF,Wibisono J,Yudhawati R,Nidom RV,Alamudi MY,Ginting TE,Makino A,Nidom CA,Shinya K,Kawaoka Y

    更新日期:2011-07-01 00:00:00