Abstract:
:A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody was mapped in the W2 domain of EBNA-LP. While another monoclonal antibody to EBNA-LP, JF186, which is widely used for analyses of the viral protein, did not react with truncated forms of EBNA-LP expressed in P3HR1 cells, as reported earlier, the LP4D3 antibody did. The LP4D3 antibody will be a useful tool for further studies of EBNA-LP, especially investigations into the phenotypes of mutant EBNA-LP expressed in P3HR1 cells.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Shaku F,Matsuda G,Furuya R,Kamagata C,Igarashi M,Tanaka M,Kanamori M,Nishiyama Y,Yamamoto N,Kawaguchi Ydoi
10.1111/j.1348-0421.2005.tb03743.xkeywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
477-83issue
5eissn
0385-5600issn
1348-0421pii
JST.JSTAGE/mandi/49.477journal_volume
49pub_type
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