Abstract:
:Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) and colA (kappa-toxin) genes were present on the same EcoRI fragment as in the standard strain, NCTC8237. Northern analysis revealed that mature plc mRNA was transcribed in KZ1340 though less efficiently than in NCTC8237, while no mature colA mRNA was present in KZ1340. After transformation of the pfoA and plc genes into the KZ1340 via shuttle vector, pJIR418, the pfoA gene was successfully expressed but the plc gene was not efficiently expressed, suggesting that in KZ1340 there is negative regulation of plc gene expression. Toxin-deficient C. perfringens KZ1340 might be a suitable host for expression analysis of the pfoA gene and other clostridial virulence genes, if expressed efficiently, because it produces a small amount of extracellular toxins.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Shimizu T,Ohtani K,Ba-Thein W,Inui S,Nakamura S,Hayashi Hdoi
10.1111/j.1348-0421.1996.tb03329.xsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
141-5issue
2eissn
0385-5600issn
1348-0421journal_volume
40pub_type
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