Antigen 85A and mycobacterial DNA-binding protein 1 are targets of immunoglobulin G in individuals with past tuberculosis.

abstract：:Of the various virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa, the type III secretion system (TTSS) has been characterized as a major factor associated with acute lung injury, bacteremia and mortality. In addition, PcrV, a component protein of the TTSS, has been characterized as a protective...

journal_title：Microbiology and immunology

authors： Yasumoto H,Katoh H,Kinoshita M,Shimizu M,Hamaoka S,Akiyama K,Naito Y,Sawa T

更新日期：2016-02-01 00:00:00

abstract：:A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine af...

journal_title：Microbiology and immunology

authors： Guo X,Jia H,Zhang Q,Yuan W,Zhu G,Xin T,Zhu H,Sánchez-Vizcaíno JM

更新日期：2012-05-01 00:00:00

abstract：:The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...

journal_title：Microbiology and immunology

authors： Kuwae T,Kurata M

更新日期：1984-01-01 00:00:00

abstract：:The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of ...

journal_title：Microbiology and immunology

authors： Iizawa Y,Nakao M,Kondo M,Yamazaki T

更新日期：1990-01-01 00:00:00

abstract：:Immune responses to Helicobacter pylori infection play important roles in gastroduodenal diseases. The contributions of tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) to the induction of gastric inflammation and to the protection from H. pylori infection were investigated using TNF-alpha gene...

journal_title：Microbiology and immunology

authors： Yamamoto T,Kita M,Ohno T,Iwakura Y,Sekikawa K,Imanishi J

更新日期：2004-01-01 00:00:00

abstract：:Phage TP1, induced from Vibrio parahaemolyticus K-20 pilot strain by mitomycin C, exhibited a unique hexagonal head with knob-like projections which covered the whole capsid and a noncontractile tail. The appearance of this phage was very similar to those of phages VP3 and VP6, isolated from seawater. The host range o...

journal_title：Microbiology and immunology

authors： Koga T,Kawata T

更新日期：1991-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:Escherichia coli isolates from calves with diarrhea (1 day to 8 weeks old, 140 individuals) were surveyed for the three immunologically distinct fimbrial adhesins FY, 31A, and K99. Of a total of 1,370 strains isolated, 96 (7.0%), 34 (2.5%), 75 (5.5%), and 13 (0.9%) were identified as FY+, 31A+, FY+.31A+, and K99+, res...

journal_title：Microbiology and immunology

authors： Shimizu M,Sakano T,Yamamoto J,Kitajima K

更新日期：1987-01-01 00:00:00

abstract：:We evaluated the efficacy of three SARS vaccine candidates in a murine SARS model utilizing low-virulence Pp and SARS-CoV coinfection. Vaccinated mice were protected from severe respiratory disease in parallel with a low virus titer in the lungs and a high neutralizing antibody titer in the plasma. Importantly, the ad...

journal_title：Microbiology and immunology

authors： Ishii K,Hasegawa H,Nagata N,Ami Y,Fukushi S,Taguchi F,Tsunetsugu-Yokota Y

更新日期：2009-02-01 00:00:00

abstract：:Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumo...

journal_title：Microbiology and immunology

authors： Fan H,Guo JY,Ma SL,Zhang N,An CL

更新日期：2016-06-01 00:00:00

abstract：:Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been ...

journal_title：Microbiology and immunology

authors： Hatch WO,Scalarone GM

更新日期：2013-11-01 00:00:00

abstract：:We found that a sub-lethal concentration of hydrogen peroxide (HPOx) enhanced the growth of Helicobacter pylori in Brucella broth supplemented with 10% fetal bovine serum (BB/FBS). The enhancement was evident at 0.1 mM HPOx and reached a maximun at 3.5 mM. The growth stimulation was dependent on the basal media used; ...

journal_title：Microbiology and immunology

authors： Murano A,Miyake M,Kato J,Tanzawa H,Takeo K,Noda M

更新日期：1999-01-01 00:00:00

abstract：:Heat resistance at 95 C, heat activation at 75 C, and germination response were determined for spores of 10 serotype strains of Clostridium perfringens type A, including five heat-resistant and five heat-sensitive strains. The D95-values ranged from 17.6 to 63.0 and from 1.3 to 2.8 for the heat-resistant and the heat-...

journal_title：Microbiology and immunology

authors： Ando Y,Tsuzuki T,Sunagawa H,Oka S

更新日期：1985-01-01 00:00:00

abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits,...

journal_title：Microbiology and immunology

authors： Nishioka M,Aibiki T,Shirai M,Terada S,Kagawa H,Watanabe S

更新日期：1986-01-01 00:00:00

abstract：:Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...

journal_title：Microbiology and immunology

authors： Wada K,Nagai H,Hagiwara T,Ibe S,Utsumi M,Kaneda T

更新日期：2004-01-01 00:00:00

abstract：:A monoclonal antibody (MoAb) to human neutrophils, designated 3H9, was established by screening for the inhibition of neutrophil adherence to plastic plates containing a medium supplemented with fetal calf serum (FCS medium). The antigen recognized by 3H9 was shown to be present on human leukocytes and found at the hi...

journal_title：Microbiology and immunology

authors： Ohtake K,Takei H,Watanabe T,Sato Y,Yamashita T,Sudo K,Kuroki M,Chihara J,Sendo F

更新日期：1997-01-01 00:00:00

abstract：:The population levels of intestinal microflora and bile acid composition in the digestive tract were examined in rats fed bile acids to determine the relationships between gastrointestinal microflora and the host. The population level of Bacteroides was increased in the ceca of rats fed cholic acid or deoxycholic acid...

journal_title：Microbiology and immunology

authors： Sakai K,Makino T,Kawai Y,Mutai M

更新日期：1980-01-01 00:00:00

abstract：:Capture enzyme-linked immunosorbent assays (ELISAs) were developed to detect immunoglobulin G and M antibodies to group A streptococcal (GAS) antigens, streptolysin O, streptokinase, and group A carbohydrate. The sensitivities and the specificities of the IgM capture ELISAs to each GAS antigen were high enough to dist...

journal_title：Microbiology and immunology

authors： Hayashi S,Yokota K,Takizawa Y,Tomizawa I,Nejime T,Oguma K

更新日期：1993-01-01 00:00:00

abstract：:A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

journal_title：Microbiology and immunology

authors： Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

更新日期：1987-01-01 00:00:00

abstract：:The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences fla...

journal_title：Microbiology and immunology

authors： Tabuchi A

更新日期：1985-01-01 00:00:00

abstract：:Water-insoluble glucan (WIG) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. Glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze WIG. The pu...

journal_title：Microbiology and immunology

authors： Otsuka R,Imai S,Murata T,Nomura Y,Okamoto M,Tsumori H,Kakuta E,Hanada N,Momoi Y

更新日期：2015-01-01 00:00:00

abstract：:Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphop...

journal_title：Microbiology and immunology

authors： Tsuda Y,Mori Y,Abe T,Yamashita T,Okamoto T,Ichimura T,Moriishi K,Matsuura Y

更新日期：2006-01-01 00:00:00

abstract：:The production from murine resident peritoneal macrophages (M phi) of a soluble factor, which was capable of enhancing the antigen-presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M phi (M phi sup) were prepared by culturing peritoneal M phi with particles, i.e., zymosan A...

journal_title：Microbiology and immunology

authors： Miyazaki H,Ito A,Osawa T

更新日期：1988-01-01 00:00:00

abstract：:The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...

journal_title：Microbiology and immunology

authors： Murakami T

更新日期：2008-05-01 00:00:00

abstract：:A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin-poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18-48 hr after endotoxin injection than in the control mice. On the ot...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Abe H,Sakaguchi O

更新日期：1984-01-01 00:00:00

abstract：:CD4(+) T cells play critical roles in protection against the blood stage of malarial infection; however, their uncontrolled activation can be harmful to the host. In this study, in which rodent models of Plasmodium parasites were used, the expression of inhibitory receptors on activated CD4(+) T cells and their cytoki...

journal_title：Microbiology and immunology

authors： Doe HT,Kimura D,Miyakoda M,Kimura K,Akbari M,Yui K

更新日期：2016-02-01 00:00:00

abstract：:Murine B cell mitogens such as bacterial lipopolysaccharide (LPS), butanol-extracted water soluble adjuvant (Bu-WSA), dextran sulfate (DS), synthetic muramyl dipeptide (MDP), and its analog MDP-Lys (L18) do not show any mitogenic ability in vitro on human peripheral blood lymphocytes or mixed cell populations of purif...

journal_title：Microbiology and immunology

authors： Nitta T,Konno-Ejiri H,Okumura S,Ozawa A,Nakano M

更新日期：1985-01-01 00:00:00

abstract：:Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

journal_title：Microbiology and immunology

authors： Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

更新日期：1993-01-01 00:00:00

abstract：:Filamentous phage, fs1, was obtained from Vibrio cholerae O139. The lysogenized strains produced a large amount of fs1 phage in the culture supernatant. This phage was previously reported as novel fimbriae of that organism. The genome of the phage was a 6.5 kb single-stranded DNA. The capsid of fsl consists of a small...

journal_title：Microbiology and immunology

authors： Nakasone N,Honma Y,Toma C,Yamashiro T,Iwanaga M

更新日期：1998-01-01 00:00:00