Abstract:
:A monoclonal antibody (MoAb) to human neutrophils, designated 3H9, was established by screening for the inhibition of neutrophil adherence to plastic plates containing a medium supplemented with fetal calf serum (FCS medium). The antigen recognized by 3H9 was shown to be present on human leukocytes and found at the highest levels on granulocytes. On Western blotting, 3H9 reacted with a molecule having a molecular weight of 80 kDa. When this MoAb was added at the same time as a neutrophil stimulant (fMLP), the inhibition of neutrophil adherence to plastic plates in the presence of FCS medium was observed after 60 min incubation. Furthermore, this MoAb enhanced not only fMLP-induced chemotaxis but random migration of neutrophils as well. The mechanisms of these phenomena are discussed.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Ohtake K,Takei H,Watanabe T,Sato Y,Yamashita T,Sudo K,Kuroki M,Chihara J,Sendo Fdoi
10.1111/j.1348-0421.1997.tb01174.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
67-72issue
1eissn
0385-5600issn
1348-0421journal_volume
41pub_type
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