Abstract:
:We established an assay system for detecting T cell-replacing factor (TRF) activity of translated materials in Xenopus oocytes of poly (A)-positive mRNA extracted from a T cell hybrid cell line, B151K12 (B151) which constitutively produces TRF. Since it was difficult to detect TRF activity of the translated products of B151-mRNA, partly because of low TRF activities, we developed the following two systems. First, RNA was prepared from B151 cells stimulated with phorbol myristate acetate and calcium ionophore A23187 because such stimulations augmented TRF production by approximately three to five-fold. Second, interleukin 2 (IL-2, 125 U/ml) was added to the culture of BCL1 cells to detect a small amount of TRF-active materials since IL-2 synergizes with a suboptimal dose of TRF to induce IgM secretion in TRF-responding BCL1 cells (chronic B cell leukemic cells). Here we describe TRF activity of translation products of B151-mRNA in Xenopus oocytes. B151-TRF mRNA was detected in the fractions sedimented between 15 and 18S by analysis using sucrose density gradient centrifugation.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Tominaga A,Matsumoto M,Takahashi T,Harada N,Takatsu Kdoi
10.1111/j.1348-0421.1986.tb03005.xsubject
Has Abstractpub_date
1986-01-01 00:00:00pages
789-98issue
8eissn
0385-5600issn
1348-0421journal_volume
30pub_type
杂志文章abstract::Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphop...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2006.tb03789.x
更新日期:2006-01-01 00:00:00
abstract::CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01091.x
更新日期:1996-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03155.x
更新日期:1987-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12354
更新日期:2016-02-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1986.tb00953.x
更新日期:1986-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1990.tb01003.x
更新日期:1990-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb01230.x
更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1993.tb03198.x
更新日期:1993-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03102.x
更新日期:1987-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
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doi:10.1111/j.1348-0421.2005.tb03695.x
更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2006.tb03861.x
更新日期:2006-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02414.x
更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1978.tb00427.x
更新日期:1978-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
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更新日期:2015-02-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1983.tb00639.x
更新日期:1983-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1993.tb03193.x
更新日期:1993-01-01 00:00:00
abstract::Congenitally athymic BALB/cA nu/nu mice were employed to elucidate the role of the thymus in experimental Mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. Chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. Macroscopic...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1979.tb00537.x
更新日期:1979-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1986.tb03014.x
更新日期:1986-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2001.tb01261.x
更新日期:2001-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00746.x
更新日期:1984-01-01 00:00:00
abstract::Borna disease virus (BoDV), a prototype of mammalian bornavirus, is a non-segmented, negative strand RNA virus that often causes severe neurological disorders in infected animals, including horses and sheep. Unique among animal RNA viruses, BoDV transcribes and replicates non-cytopathically in the cell nucleus, leadin...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
abstract::Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12367
更新日期:2016-04-01 00:00:00