Anti-viral and anti-bacterial activities of an extract of blackcurrants (Ribes nigrum L.).

Abstract:

:The inhibitory effects of an extract of the blackcurrant (Ribes nigrum L.) against pathogens associated with oral, nasopharyngeal and upper respiratory infectious diseases; namely respiratory syncytial virus (RSV), influenza virus A and B (IFV-A and IFV-B), adenovirus (AdV), herpes simplex virus type 1, Haemophilus influenzae type B, Streptococcus pneumoniae and Streptococcus mutans, were investigated. Less than 1% concentration of extract of blackcurrant inhibited replication of RSV, IFV-A and -B and HSV-1 by over 50% and a 10% extract inhibited adsorption of these viruses onto the cell surface by over 95%. The effects on AdV were much less pronounced; the half minimal inhibitory concentration of AdV replication was 2.54 ± 0.26, and a 10% concentration of the extract inhibited AdV adsorption on the cell surface by 72.9 ± 3.4%. The antibacterial activities of the blackcurrant were evaluated based on its efficacy as a disinfectant. A 10% extract disinfected 99.8% of H. Influenzae type B and 78.9% of S. pneumoniae in 10 min, but had no demonstrable effect against S. mutans. The blackcurrant extract still showed antiviral and antibacterial activities after the pH had been made neutral with sodium hydroxide, suggesting that these activities are not the result of acidic reactions or of components precipitated at a neutral pH. These findings demonstrate the potential of blackcurrant extract as a functional food for oral care.

journal_name

Microbiol Immunol

authors

Ikuta K,Hashimoto K,Kaneko H,Mori S,Ohashi K,Suzutani T

doi

10.1111/j.1348-0421.2012.00510.x

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

805-9

issue

12

eissn

0385-5600

issn

1348-0421

journal_volume

56

pub_type

杂志文章
  • Effect of the extract made from Cochinchina momordica seeds on the humoral immune responses of mice to a commercial foot-and-mouth disease vaccine (serotypes O and Asia 1).

    abstract::The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosupp...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2009.00180.x

    authors: Sakwiwatkul K,Li RL,Song XM,Hu SH

    更新日期:2010-01-01 00:00:00

  • Diagnosis of toxic shock syndrome by two different systems; clinical criteria and monitoring of TSST-1-reactive T cells.

    abstract::Two methods of TSS diagnosis were evaluated: comparison of symptoms with clinical criteria and monitoring for evidence of selective activation of Vbeta2(+) T cells by the causative toxin, TSS toxin-1 (TSST-1). Ten patients with acute and systemic febrile infections caused by Staphylococcus aureus were monitored for in...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2008.00071.x

    authors: Matsuda Y,Kato H,Ono E,Kikuchi K,Muraoka M,Takagi K,Imanishi K,Itoh S,Itoh T,Ogawa T,Nitta K,Inokuchi S,Hibi T,Ohta H,Uchiyama T

    更新日期:2008-11-01 00:00:00

  • Clostridium botulinum type C in healthy swine in Japan.

    abstract::Healthy cattle and swine bred in a district of Japan were examined for the presence of Clostridium botulinum in their liver. Liver specimens were cultivated in chopped meat-glucose medium and the cultures were examined for botulinum toxin. In cattle, none of the cultures of 100 liver specimens yielded the toxin. In sw...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1992.tb01639.x

    authors: Yamakawa K,Kamiya S,Yoshimura K,Nakamura S

    更新日期:1992-01-01 00:00:00

  • Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts.

    abstract::Cauliflower mosaic virus (CaMV) open reading frame (ORF) VI product (P6) has been shown to be the major constituent of viral inclusion body, to function as a post-transcriptional transactivator, and to be essential for infectivity on whole plants. Although these findings suggest that P6 has an important role in viral ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1998.tb02298.x

    authors: Kobayashi K,Tsuge S,Nakayashiki H,Mise K,Furusawa I

    更新日期:1998-01-01 00:00:00

  • Epitope analysis using anti-oligosaccharide (G4) monoclonal antibody.

    abstract::A murine monoclonal antibody recognizing (1-->6)-beta-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyll...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1995.tb02166.x

    authors: Hirata A,Komoda M,Itoh W,Tabata K,Sugawara I

    更新日期:1995-01-01 00:00:00

  • Cell surface of a tetrads-forming mutant of Micrococcus luteus: chemical treatment of the cells and teichuronic acids on the surface.

    abstract::Tetrads-forming mutant MT cells of Micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. The binding of protein A-gold particles to the cel...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1990.tb00992.x

    authors: Monodane T,Tokunaga M,Torii M

    更新日期:1990-01-01 00:00:00

  • Pathogenicity of glycoprotein C-deficient herpes simplex virus 1 strain TN-1 which encodes truncated glycoprotein C.

    abstract::A clinical isolate of herpes simplex virus 1 (TN-1) from a stromal keratitis patient was found to be defective in the glycoprotein C (gC) gene (UL44), thus resulting in the production of truncated gC upon infection. To study the pathogenetic role of truncated gC, we prepared a recombinant LTN-8 derived from TN-1 with ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01890.x

    authors: Minagawa H,Liu Y,Yoshida T,Hidaka Y,Toh Y,Mori R

    更新日期:1997-01-01 00:00:00

  • Small ruminant lentiviral Vif proteins commonly utilize cyclophilin A, an evolutionarily and structurally conserved protein, to degrade ovine and caprine APOBEC3 proteins.

    abstract::Mammals have co-evolved with retroviruses, including lentiviruses, over a long period. Evidence supporting this contention is that viral infectivity factor (Vif) encoded by lentiviruses antagonizes the anti-viral action of cellular apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3 (APOBEC3) of the host...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12387

    authors: Yoshikawa R,Izumi T,Nakano Y,Yamada E,Moriwaki M,Misawa N,Ren F,Kobayashi T,Koyanagi Y,Sato K

    更新日期:2016-06-01 00:00:00

  • Oral delivery of antigens in liposomes with some lipid compositions modulates oral tolerance to the antigens.

    abstract::Several liposomes containing ovalbumin (OVA), a model antigen, with different lipid compositions were prepared in order to evaluate their ability to induce oral tolerance. Oral administration of these liposomal OVAs induced suppression of the proliferative responses of popliteal lymph node cells from the treated mice ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2002.tb02677.x

    authors: Masuda K,Horie K,Suzuki R,Yoshikawa T,Hirano K

    更新日期:2002-01-01 00:00:00

  • Genetic control of immune response to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans.

    abstract::The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01899.x

    authors: Honma K,Ishii N,Kato T,Ishihara K,Okuda K,Okuda K

    更新日期:1997-01-01 00:00:00

  • Susceptibility of influenza viruses to interferon and to poly(I) . Poly(C) determined by the plaque reduction method.

    abstract::Susceptibility of eight strains of influenza A and B viruses to interferon and to poly(I) . poly(C) were determined by the plaque reduction method. All strains tested were slightly less susceptible than vesicular stomatitis virus (VSV) in an established line of canine kidney (MDCK) cells. The 50% plaque depression dos...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1980.tb02873.x

    authors: Machida H,Kuninaka A,Yoshino H

    更新日期:1980-01-01 00:00:00

  • Moraxella (Branhamella) catarrhalis adherence to human bronchial and oropharyngeal cells: the role of adherence in lower respiratory tract infections.

    abstract::To study the role of Moraxella (subgenus Branhamella) catarrhalis (B. catarrhalis) adherence to airway cells in lower respiratory tract infections, the in vitro attachments of B. catarrhalis to upper airway (oropharyngeal) and lower airway (bronchial) epithelial cells were compared. The adherence of 4 strains (1 nonfi...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01882.x

    authors: Rikitomi N,Ahmed K,Nagatake T

    更新日期:1997-01-01 00:00:00

  • Studies on the sulfite reduction test for clostridia.

    abstract::Peptone-yeast extract (PY) medium containing 0.035% ferric ammonium citrate as an indicator, 0.05% sulfite as a substrate, 0.05% cysteine as a reducer and 0.5% glucose was found to be suitable for observing the sulfite reduction test. The effect of added cysteine on the test was suppressed by the addition of glucose. ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1980.tb02830.x

    authors: Kawabata N

    更新日期:1980-01-01 00:00:00

  • Studies on structural proteins of Chikungunya Virus. I. Separation of three species of proteins and their preliminary characterization.

    abstract::Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights wer...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1980.tb02846.x

    authors: Konishi E,Hotta S

    更新日期:1980-01-01 00:00:00

  • A unique mutation of glycoprotein gene of the attenuated RC-HL strain of rabies virus, a seed virus used for production of animal vaccine in Japan.

    abstract::Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This result suggests that besides arginine at position 333, some other positions o...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1994.tb01812.x

    authors: Ito H,Minamoto N,Watanabe T,Goto H,Rong LT,Sugiyama M,Kinjo T,Mannen K,Mifune K,Konobe T

    更新日期:1994-01-01 00:00:00

  • Mechanism of antibody-mediated protection against herpes simplex virus infection in athymic nude mice: requirement of Fc portion of antibody.

    abstract::Experiments were performed to investigate the resistance of the host due to antibody-mediated mechanisms to herpes simplex virus (HSV) infection. Transfer of hyperimmune anti-HSV mouse serum inhibited the development of skin lesions and prolonged the survival of lethally HSV-infected nude mice. Relatively high concent...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1982.tb00203.x

    authors: Hayashida I,Nagafuchi S,Hayashi Y,Kino Y,Mori R,Oda H,Ohtomo N,Tashiro A

    更新日期:1982-01-01 00:00:00

  • An ultrastructural study of HeLa cell invasion with Salmonella typhi GIFU 10007.

    abstract::Scanning electron micrograph of HeLa S3 monolayered cells, inoculated with viable bacteria of a Salmonella typhi strain GIFU 10007, revealed that the extended microvilli tangled the bacteria within 10 min after inoculation. The micrographs of HeLa cells, at 1 hr after inoculation, indicate the following: shortening of...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1987.tb03063.x

    authors: Yokoyama H,Ikedo M,Kohbata S,Ezaki T,Yabuuchi E

    更新日期:1987-01-01 00:00:00

  • Effect of N-acetylchito-oligosaccharides on activation of phagocytes.

    abstract::Four N-acetylchito-oligosaccharides, from tetra-N-acetylchitotetraose (NACOS-4) to hepta-N-acetylchitoheptaose (NACOS-7), were found to increase the number of peritoneal exudate cells (PEC) in male BALB/c mice after 3 hr intraperitoneal administration of 50 mg/kg of each oligosaccharide. The number of attracted cells,...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1986.tb03004.x

    authors: Suzuki K,Tokoro A,Okawa Y,Suzuki S,Suzuki M

    更新日期:1986-01-01 00:00:00

  • Activation of Toll‐like receptor 9 inhibits lipopolysaccharide‐induced receptor activator of nuclear factor kappa‐ B ligand expression in rat B lymphocytes.

    abstract::B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12129

    authors: Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

    更新日期:2014-01-01 00:00:00

  • Apoptosis observed in murine peritoneal macrophages treated with interferon gamma through staphylococcal enterotoxin-dependent cell-mediated cytotoxicity.

    abstract::The concept of superantigens is well-known and widely accepted. In this brief communication, we analyze the behaviour of antigen-presenting cells after T-cell activation by staphylococcal enterotoxin B, a representative superantigen. We tried to activate murine T cells by inflammatory mouse peritoneal macrophage in th...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb02603.x

    authors: Sakurada J,Tajima A,Shinji H,Seki K,Masuda S

    更新日期:2000-01-01 00:00:00

  • Enhanced effect of BCG vaccine against pulmonary Mycobacterium tuberculosis infection in mice with lung Th17 response to mycobacterial heparin-binding hemagglutinin adhesin antigen.

    abstract::Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12340

    authors: Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

    更新日期:2015-12-01 00:00:00

  • Endogenous inhibitors of factor B.

    abstract::proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1983.tb02941.x

    authors: Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

    更新日期:1983-01-01 00:00:00

  • Physiological roles of three Na+/H+ antiporters in the halophilic bacterium Vibrio parahaemolyticus.

    abstract::Vibrio parahaemolyticus mutants lacking three Na+/H+ antiporters (NhaA, NhaB, NhaD) were constructed. The DeltanhaA strains showed significantly higher sensitivity to LiCl regarding their growth compared to the parental strain. The DeltanhaA and DeltanhaB strains exhibited higher sensitivities to LiCl. The mutant XACa...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2005.tb03662.x

    authors: Kuroda T,Mizushima T,Tsuchiya T

    更新日期:2005-01-01 00:00:00

  • Induction of acute arthritis in mice by peptidoglycan derived from gram-positive bacteria and its possible role in cytokine production.

    abstract::The activities of a water-soluble peptidoglycan fragment derived from Staphylococcus epidermidis (SEPS) were examined as to their role in proliferation of spleen mononuclear cells (SMNC) from various strains of mice, the production of cytokines in vitro, and the induction of an inflammatory reaction in vivo. The proli...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb01679.x

    authors: Onta T,Sashida M,Fujii N,Sugawara S,Rikiishi H,Kumagai K

    更新日期:1993-01-01 00:00:00

  • Herpes simplex virus-specific IgM, IgA and IgG subclass antibody responses in primary and nonprimary genital herpes patients.

    abstract::To clarify the humoral immunity in herpes simplex virus (HSV) infection, HSV-specific IgM, IgA and IgG subclass antibody responses were studied in patients with genital herpes: 17 primary, 13 recurrent and 6 nonprimary first episode. A total of 181 serum samples serially collected from the patients, 5 per patient unti...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01872.x

    authors: Hashido M,Kawana T

    更新日期:1997-01-01 00:00:00

  • Temperature-sensitive immunoglobulin A-binding and dimerization of C-terminus-impaired protein Arp4 produced in Escherichia coli.

    abstract::A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1992.tb02096.x

    authors: Nakatani T,Higuchi A,Noguchi H

    更新日期:1992-01-01 00:00:00

  • Resistance of Pseudomonas pseudomallei to normal human serum bactericidal action.

    abstract::The effect of human normal serum (HNS) on Pseudomonas pseudomallei was determined. It is apparent from our data that the organism is resistant to the normal serum bactericidal mechanism. Ancillary experiments to confirm this serum-resistant property of P. pseudomallei were done by examining the effects of growth phase...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1988.tb01426.x

    authors: Ismail G,Razak N,Mohamed R,Embi N,Omar O

    更新日期:1988-01-01 00:00:00

  • Inhibition of HIV-1 infection by an intramolecular antisense peptide to T20 in gp160.

    abstract::Antisense amino acids are amino acids which can be translated from the corresponding anti-codons of a sense amino acid. Antisense peptides encoded by the noncoding DNA strand have a tendency to interact with each other. We have demonstrated that antisense peptide sequences are present intramolecularly, and these may c...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb02485.x

    authors: Imai M,Okada N,Okada H

    更新日期:2000-01-01 00:00:00

  • Genotypic characterization of human and environmental isolates of Salmonella choleraesuis subspecies choleraesuis serovar infantis by pulsed-field gel electrophoresis.

    abstract::To determine the extent of genetic diversity of Salmonella choleraesuis subspecies choleraesuis serovar Infantis and whether environmental isolates were similar or identical to human isolates, a total of 110 isolates from humans, broiler samples, egg production facilities, riverwater, sewage, and chicken meat were ana...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1999.tb02406.x

    authors: Murakami K,Horikawa K,Otsuki K

    更新日期:1999-01-01 00:00:00

  • Epidemiological analysis of serum anti-Pseudomonas aeruginosa PcrV titers in adults.

    abstract::Of the various virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa, the type III secretion system (TTSS) has been characterized as a major factor associated with acute lung injury, bacteremia and mortality. In addition, PcrV, a component protein of the TTSS, has been characterized as a protective...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12353

    authors: Yasumoto H,Katoh H,Kinoshita M,Shimizu M,Hamaoka S,Akiyama K,Naito Y,Sawa T

    更新日期:2016-02-01 00:00:00