Abstract:
:To study the role of Moraxella (subgenus Branhamella) catarrhalis (B. catarrhalis) adherence to airway cells in lower respiratory tract infections, the in vitro attachments of B. catarrhalis to upper airway (oropharyngeal) and lower airway (bronchial) epithelial cells were compared. The adherence of 4 strains (1 nonfimbriated and 3 fimbriated) of B. catarrhalis to respiratory tract epithelial cells collected from 11 patients with chronic pulmonary disease (CPD) and 11 healthy individuals was evaluated. Both the fimbriated and nonfimbriated strains showed increased attachment to oropharyngeal cells in the CPD patients (mean +/- SEM; 25.0 +/- 3.2/cell; P < 0.01) when compared to the control subjects (12.1 +/- 1.1/cell). On the average, the attachment to bronchial cells was 6.1 to 13.6 times greater per surface area (bacteria/micron2) than the attachment to oropharyngeal cells. The fimbriated strains tended to adhere in higher numbers to bronchial cells (19.0 +/- 1.8/cell) than the nonfimbriated strain (8.7 +/- 1.2/cell), although there was no difference between the CPD and control groups. In conclusion, the attachment of B. catarrhalis to oropharyngeal cells may be an enhancing factor for colonization in the upper respiratory tract in patients with CPD, and elevated adherence of the bacteria to bronchial cells may suggest pathogenic importance when mucociliary function is impaired.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Rikitomi N,Ahmed K,Nagatake Tdoi
10.1111/j.1348-0421.1997.tb01882.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
487-94issue
6eissn
0385-5600issn
1348-0421journal_volume
41pub_type
杂志文章abstract::Several liposomes containing ovalbumin (OVA), a model antigen, with different lipid compositions were prepared in order to evaluate their ability to induce oral tolerance. Oral administration of these liposomal OVAs induced suppression of the proliferative responses of popliteal lymph node cells from the treated mice ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2002.tb02677.x
更新日期:2002-01-01 00:00:00
abstract::The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1986.tb02970.x
更新日期:1986-01-01 00:00:00
abstract::In order to analyze the host-parasite interactions in experimental trichomoniasis, the growth of Trichomonas foetus in the peritoneal cavity and changes in the peritoneal exudate cells were followed in mice treated with dextran sulfate 500 (DS 500), a known macrophage-toxic agent. Light microscopic observation showed ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1980.tb02898.x
更新日期:1980-01-01 00:00:00
abstract::The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...
journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.2008.00008.x
更新日期:2008-05-01 00:00:00
abstract::Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03102.x
更新日期:1987-01-01 00:00:00
abstract::Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expre...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12079
更新日期:2013-09-01 00:00:00
abstract::Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03272.x
更新日期:1995-01-01 00:00:00
abstract::To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhim...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03255.x
更新日期:1995-01-01 00:00:00
abstract::Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01472.x
更新日期:1988-01-01 00:00:00
abstract::Porphyromonas gingivalis is a pathogen associated with adult periodontitis, which is a chronic inflammatory disease characterized by breakdown of the periodontal tissue. Dipeptidyl aminopeptidase IV (DPPIV) produced by P. gingivalis has been considered to be a potential virulence factor based on the finding that the v...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03443.x
更新日期:2003-01-01 00:00:00
abstract::Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00696.x
更新日期:1984-01-01 00:00:00
abstract::L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in bo...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01754.x
更新日期:1994-01-01 00:00:00
abstract::A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2011.00406.x
更新日期:2012-02-01 00:00:00
abstract::We examined the mechanisms of fluconazole resistance in a fluconazole-resistant Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis. It was demonstrated that the highly resistant phenotype of this strain was associated with combined mechanisms of the energy-dependent reduced intrace...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2004.tb03623.x
更新日期:2004-01-01 00:00:00
abstract::The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb02963.x
更新日期:1985-01-01 00:00:00
abstract::We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antise...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01431.x
更新日期:1988-01-01 00:00:00
abstract::There are many examples of probiotic effects of various lactic bacteria on enteropathogens. In this study, Lactobacillus strains (L. rhamnosus, L. gasseri, L. casei and L. plantarum) were tested in an in vitro model of enterohemorrhagic Escherichia coli (EHEC) infection of a human colon epithelial cell line, C2BBe1. W...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03377.x
更新日期:2003-01-01 00:00:00
abstract::Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12249
更新日期:2015-05-01 00:00:00
abstract::Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utiliz...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01212.x
更新日期:1997-01-01 00:00:00
abstract::For the simple and rapid detection/identification of major pathogenic fungal species such as Candida albicans, C. tropicalis, C. parapsilosis, C. glabrata and Aspergillus fumigatus, common primers for these species and specific primers for each species, designed on the basis on the genomic nucleotide sequences of the ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03426.x
更新日期:2003-01-01 00:00:00
abstract::The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03251.x
更新日期:1995-01-01 00:00:00
abstract::Fibronectin is known to be an important factor in colonization by Staphylococcus aureus of host tissues as well as other extracellular matrix proteins such as collagen and laminin. We investigated the effect of fibronectin on the phagocytosis of the S. aureus Cowan I strain by macrophages and of coagulase-negative sta...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02361.x
更新日期:1998-01-01 00:00:00
abstract::The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences fla...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00839.x
更新日期:1985-01-01 00:00:00
abstract::The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2004.tb03503.x
更新日期:2004-01-01 00:00:00
abstract::Unmethylated CpG-ODN are known to enhance Th1-type immune response. However, optimal sequences of CpG-ODN for activating Th1-type immune cells vary among species. It is necessary to identify the effective CpG-ODN sequences in each species. In the present study, in order to identify the sequences of CpG-ODN that produc...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2010.00298.x
更新日期:2011-03-01 00:00:00
abstract::A 4-year (1996-2000) survey of rotavirus infection involving 2,218 diarrheal fecal specimens of children collected from five regions of Japan was conducted. A total of 642 (28.9%) specimens were found to be rotavirus positive. A changed prevalence pattern of rotavirus G serotype was found with an increase of G9 and G2...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03422.x
更新日期:2003-01-01 00:00:00
abstract::A new type of immuno-cell therapy called BRM-activated killer (BAK) therapy using non-MHC-restricted lymphocytes, CD56-positive cells, was devised. Peripheral blood lymphocytes were selected by immobilization with anti-CD3 monoclonal antibody and cultured for 2 weeks in the presence of IL-2. Thereafter, they were reac...
journal_title:Microbiology and immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1348-0421.2001.tb02638.x
更新日期:2001-01-01 00:00:00
abstract::Autoantibodies to myeloperoxidase (MPO) are a subset of anti-neutrophil cytoplasmic antibody (ANCA, MPO-ANCA) detected in the sera of some patients with primary systemic vasculitis. The titer of MPO-ANCA does not always reflect disease activity and this inconsistency may be attributable to differences in epitopic spec...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb04017.x
更新日期:2007-01-01 00:00:00
abstract::EAEC is increasingly recognized as an emerging enteric pathogen. Typical EAEC expressing the AggR regulon have been proven to be an important cause of childhood diarrhea in industrialized countries as well as in the developing world, while atypical EAEC without this regulon have not been thoroughly investigated. To in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2010.00210.x
更新日期:2010-06-01 00:00:00
abstract::We evaluated the efficacy of three SARS vaccine candidates in a murine SARS model utilizing low-virulence Pp and SARS-CoV coinfection. Vaccinated mice were protected from severe respiratory disease in parallel with a low virus titer in the lungs and a high neutralizing antibody titer in the plasma. Importantly, the ad...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2008.00097.x
更新日期:2009-02-01 00:00:00