A focus assay method for Japanese encephalitis virus using complement and anti-virus serum.

abstract：:The gH of CMV is a major target for strain-specific neutralizing antibodies. To verify whether there is a correlation between HLA-DR type and strain-specific antibodies, antibodies against CMV gH in potential donors and recipients for renal transplantation were investigated. Among 471 subjects, 404 (86%) showed reacti...

journal_title：Microbiology and immunology

authors： Ishibashi K,Tokumoto T,Shirakawa H,Hashimoto K,Kushida N,Yanagida T,Shishido K,Aikawa K,Yamaguchi O,Toma H,Tanabe K,Suzutani T

更新日期：2009-07-01 00:00:00

abstract：:The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

journal_title：Microbiology and immunology

authors： Singh JP,Verma R

更新日期：2008-08-01 00:00:00

abstract：:Diarrheal diseases are major causes of morbidity and mortality among children in developing countries. We have analyzed the causative agents of diarrhea in children under five years of age who resided in rural environments but attended a hospital in Malindi, a coastal town in Kenya. Bacterial diarrhea was found in 239...

journal_title：Microbiology and immunology

authors： Saidi SM,Iijima Y,Sang WK,Mwangudza AK,Oundo JO,Taga K,Aihara M,Nagayama K,Yamamoto H,Waiyaki PG,Honda T

更新日期：1997-01-01 00:00:00

abstract：:The expression of herpes simplex virus (HSV) type-common surface antigens (CSA) in a representative cell clone (155-4-03) of hamster cell line 155-4 transformed by HSV type 2 was enhanced by treatment with inhibitors of RNA synthesis [adriamycin (ADM) and daunomycin] but not with inhibitors of DNA synthesis (2-iododeo...

journal_title：Microbiology and immunology

authors： Kako D,Ohnishi Y,Kimura S

更新日期：1981-01-01 00:00:00

abstract：:The colH gene encoding 116-kDa collagenase of Clostridium histolyticum (cColH) was cloned into an Escherichia coli-Bacillus subtilis shuttle vector to develop a method for purification of recombinant collagenase (rColH). When plasmid pJCM310 containing the colH gene was introduced into B. subtilis DB104 and the transf...

journal_title：Microbiology and immunology

authors： Jung CM,Matsushita O,Katayama S,Minami J,Ohhira I,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into Vero...

journal_title：Microbiology and immunology

authors： Kee SH,Cho EJ,Song JW,Park KS,Baek LJ,Song KJ

更新日期：2004-01-01 00:00:00

abstract：:We determined the effect of heat-stable enterotoxin produced by Yersinia enterocolitica (Y. enterocolitica ST) on cyclic nucleotide levels in the intestines of 6-day-old mice and in cultured cell line cells. The concentration of cyclic guanosine 3',5'-monophosphate (cyclic GMP) in homogenates of the intestines increas...

journal_title：Microbiology and immunology

authors： Inoue T,Okamoto K,Moriyama T,Takahashi T,Shimizu K,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:Absorption of a pooled human gamma globulin preparation with acetone-treated measles virus-infected cells removed all antibodies to measles virus antigens except a portion of the antibody to the fusion (F) protein. The residual anti-F antibody had hemolysis-inhibiting and virus-neutralizing activities, inhibited sprea...

journal_title：Microbiology and immunology

authors： Sato TA,Kohama T,Sugiura A

更新日期：1989-01-01 00:00:00

abstract：:One strain each of granular and downy varieties of Trichophyton mentagrophytes and two strains each of granular and floccose colonial types Trichophyton rubrum were used for preparation of antigens. The antigens were analyzed by double diffusion against homologous and heterologous antisera raised in rabbits. A total o...

journal_title：Microbiology and immunology

authors： Shukla NP,Agarwal GP

更新日期：1983-01-01 00:00:00

abstract：:Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...

journal_title：Microbiology and immunology

authors： Shinoda S,Kobayashi M,Yamada H,Yoshida S,Ogawa M,Mizuguchi Y

更新日期：1987-01-01 00:00:00

abstract：:Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an ass...

journal_title：Microbiology and immunology

authors： Kuramitsu M,Okuma K,Nakashima M,Sato T,Sasaki D,Hasegawa H,Umeki K,Kubota R,Sasada K,Sobata R,Matsumoto C,Kaneko N,Tezuka K,Matsuoka S,Utsunomiya A,Koh KR,Ogata M,Ishitsuka K,Taki M,Nosaka K,Uchimaru K,Iwanaga M

更新日期：2018-10-01 00:00:00

abstract：:This study proposes a novel chemiluminescent assay of bacterial activity. Luminol chemiluminescence (LC) was amplified on addition of menadione to Escherichia coli suspension, and it was effectively inhibited by addition of superoxide dismutase rather than catalase. This fact suggests that H2O2 produced from O2 by sup...

journal_title：Microbiology and immunology

authors： Yamashoji S,Manome I,Ikedo M

更新日期：2001-01-01 00:00:00

abstract：:In this review, we have emphasized: 1) bacterial lipopolysaccharide (LPS) involvement in IgA responses to orally administered thymic-dependent (TD) antigens; 2) characterization of Peyer's patch (PP) lymphoreticular cells; and 3) gastrointestinal immunization with gram negative pathogens and anti-LPS immunity to infec...

journal_title：Microbiology and immunology

authors： McGhee JR,Michalek SM,Kiyono H,Eldridge JH,Colwell DE,Williamson SI,Wannemuehler MJ,Jirillo E,Mosteller LM,Spalding DM

更新日期：1984-01-01 00:00:00

abstract：:Human adenoviruses (HAdVs) are an important cause of acute gastroenteritis in children. However, few studies on the epidemiology or types of HAdVs associated with acute gastroenteritis have been conducted in Korea. Therefore, in the present study, the incidence of HAdV in 2064 stool samples from Korean children hospit...

journal_title：Microbiology and immunology

authors： Lee JI,Lee GC,Chung JY,Han TH,Lee YK,Kim MS,Lee CH

更新日期：2012-08-01 00:00:00

abstract：:Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

journal_title：Microbiology and immunology

authors： Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

更新日期：2000-01-01 00:00:00

abstract：:We elucidated the contribution of Valpha14 NKT cells to Th1 response and host resistance against mycobacterial infection. In Valpha14 NKT cell-deficient mice, host defense and DTH response to Mycobacterium bovis BCG were not different from wild-type mice after pulmonary infection. There was no significant difference i...

journal_title：Microbiology and immunology

authors： Kawakami K,Kinjo Y,Uezu K,Yara S,Miyagi K,Koguchi Y,Nakayama T,Taniguchi M,Saito A

更新日期：2002-01-01 00:00:00

abstract：:Mammals have co-evolved with retroviruses, including lentiviruses, over a long period. Evidence supporting this contention is that viral infectivity factor (Vif) encoded by lentiviruses antagonizes the anti-viral action of cellular apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3 (APOBEC3) of the host...

journal_title：Microbiology and immunology

authors： Yoshikawa R,Izumi T,Nakano Y,Yamada E,Moriwaki M,Misawa N,Ren F,Kobayashi T,Koyanagi Y,Sato K

更新日期：2016-06-01 00:00:00

abstract：:Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

journal_title：Microbiology and immunology

authors： Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

更新日期：2008-12-01 00:00:00

abstract：:Both cluster of differentiation (CD)4(+) and CD8(+) T lymphocytes play key roles in immunity to Brucella, in part because they secrete interferon (IFN)-γ and activate bactericidal functions in macrophages. Therefore, use of markers of macrophage activation may have diagnostic and prognostic significance. High-mobili...

journal_title：Microbiology and immunology

authors： Ayarcı AO,Yılmaz E,Sığırlı D,Budak F,Göral G,Oral HB

更新日期：2013-02-01 00:00:00

abstract：:We found that mycoplasma-infected cells have a higher ability to metastasize in vivo than non-mycoplasma-infected cells. To investigate this phenomenon, we obtained a monoclonal antibody, MAb 243-5, by immunization with Mycoplasma arginini-infected RPMI 4788 cells. This MAb recognized a mycoplasmal protein with an MW ...

journal_title：Microbiology and immunology

authors： Ushio S,Iwaki K,Taniai M,Ohta T,Fukuda S,Sugimura K,Kurimoto M

更新日期：1995-01-01 00:00:00

abstract：:The fusion of BHK-21-KB cells by vesicular stomatitis virus was not induced in Eagle's minimal essential medium without glucose. In medium containing glucose, the rate of polykaryocyte formation decreased as the concentration of glucose was reduced below 5 mM. However, no reduction in virus production 24 hr after infe...

journal_title：Microbiology and immunology

authors： Takehara M

更新日期：1981-01-01 00:00:00

abstract：:Normal, thioglycollate-stimulated and BCG-activated mouse peritoneal macrophages were cultivated in vitro with the conditioned medium of mouse L-929 cells. The thioglycollate- and BCG-macrophages rapidly proliferated, whereas normal macrophages grew more slowly. A clear morphological difference between the three types...

journal_title：Microbiology and immunology

authors： Nozawa RT,Yanaki N,Yokota T

更新日期：1980-01-01 00:00:00

abstract：:A leukocidin was isolated and purified from autolysates of Pseudomonas aeruginosa strain 158 by a combination of procedures such as column chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-100, and zone electrophoresis on pevikon. The purified preparation showed a single band in each experiment using ...

journal_title：Microbiology and immunology

authors： Hirayama T,Kato I,Matsuda F,Noda M

更新日期：1983-01-01 00:00:00

abstract：:To study the role of Moraxella (subgenus Branhamella) catarrhalis (B. catarrhalis) adherence to airway cells in lower respiratory tract infections, the in vitro attachments of B. catarrhalis to upper airway (oropharyngeal) and lower airway (bronchial) epithelial cells were compared. The adherence of 4 strains (1 nonfi...

journal_title：Microbiology and immunology

authors： Rikitomi N,Ahmed K,Nagatake T

更新日期：1997-01-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:Mice primed with 1 microgram of reduced and alkylated ovalbumin (RA-OA) developed not only long-lived memory cells for delayed-type hypersensitivity (DTH), capable of differentiating into DTH-effector T cells (DTH-Te) against ovalbumin (OA) when restimulated in vitro with OA, but also spleen cells capable of augmentin...

journal_title：Microbiology and immunology

authors： Kojima A,Tamura SI,Egashira Y

更新日期：1982-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:To investigate cellular components incorporated into the rabies virion, monoclonal antibodies (MAbs) were screened based on their reactivity with additional virion components. Two of the MAbs we prepared recognized a virion-associated 21 kDa polypeptide (referred to as VAP21) from a BHK-21 cell. Since the MAbs precipi...

journal_title：Microbiology and immunology

authors： Sagara J,Tochikura TS,Yamamoto T,Tsukita S,Tsukita S,Kawai A

更新日期：1997-01-01 00:00:00

abstract：:Sixteen temperature-sensitive mutants of Sendai virus were isolated from mutagenized stocks (10 mutants, designated numerically) and persistently infected cultures (6 mutants, designated alphabetically). Based on complementation tests, virion-associated activities, thermal inactivation, and viral RNA and hemadsorbing ...

journal_title：Microbiology and immunology

authors： Adachi A,Kanda T,Shibuta H

更新日期：1980-01-01 00:00:00