Menadione-catalyzed O2- production by Escherichia coli cells: application of rapid chemiluminescent assay to antimicrobial susceptibility testing.

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were...

journal_title：Microbiology and immunology

authors： Yang W,Shi L,Jia WX,Yin X,Su JY,Kou Y,Yi X,Shinoda S,Miyoshi S

更新日期：2005-01-01 00:00:00

abstract：:The botulinum neurotoxin light chain (BoNT-LC) is a zinc-dependent metalloprotease that cleaves neuronal SNARE proteins such as SNAP-25, VAMP2, and Syntaxin1. This cleavage interferes with the neurotransmitter release of peripheral neurons and results in flaccid paralysis. SNAP, VAMP, and Syntaxin are representative o...

journal_title：Microbiology and immunology

authors： Yamamoto H,Ida T,Tsutsuki H,Mori M,Matsumoto T,Kohda T,Mukamoto M,Goshima N,Kozaki S,Ihara H

更新日期：2012-04-01 00:00:00

abstract：:Macrophages act as a reservoir for Mycobacterium tuberculosis, producing latent infection in approximately 90% of infected people. In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during infection with the most relevant mycobacterial strains for humans (M. tuberculosis, M. bov...

journal_title：Microbiology and immunology

authors： Elizabeth MC,Hernández de la Cruz ON,Mauricio CA

更新日期：2016-05-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:The in vitro effect of the heat-labile toxin (HLT) of Bordetella parapertussis on HeLa, baby hamster kidney (BHK), chinese hamster ovary (CHO), myeloma (Pa-NS-1), human embryonic lung (HEL-R66) cells, erythrocytes, adipocytes and lymphocytes from guinea pigs, mice or rats, or aortic smooth muscle cells from pigs or gu...

journal_title：Microbiology and immunology

authors： Endoh M,Nagai M,Ueda T,Yoshida Y,Nakase Y

更新日期：1988-01-01 00:00:00

abstract：:This study aimed to investigate the antifungal activity of Rubus chingii extract in combination with fluconazole (FLC) against FLC-resistant Candida albicans 100 in vitro. A R. chingii extract and FLC-resistant C. albicans fungus suspension were prepared. The minimum inhibitory concentration and fractional inhibitory ...

journal_title：Microbiology and immunology

authors： Han B,Chen J,Yu YQ,Cao YB,Jiang YY

更新日期：2016-02-01 00:00:00

abstract：:Measles vaccines are highly effective and safe; however, the mechanism(s) underlying their attenuation has not been well understood. In this study, type I IFNs (IFN-α and IFN-β) induction in macaques infected with measles virus (MV) strains was examined. Type I IFNs were not induced in macaques infected with wild-type...

journal_title：Microbiology and immunology

authors： Van Nguyen N,Kato SI,Nagata K,Takeuchi K

更新日期：2016-07-01 00:00:00

abstract：:Porphyromonas gingivalis is a pathogen associated with adult periodontitis, which is a chronic inflammatory disease characterized by breakdown of the periodontal tissue. Dipeptidyl aminopeptidase IV (DPPIV) produced by P. gingivalis has been considered to be a potential virulence factor based on the finding that the v...

journal_title：Microbiology and immunology

authors： Kumagai Y,Yajima A,Konishi K

更新日期：2003-01-01 00:00:00

abstract：:Aged C57BL/6 (B6) mice could reject allogeneic BALB/c RL male 1 tumor as efficiently as young B6 mice. However, in vitro analysis showed impaired generation of cytotoxic T cell response in aged B6 mice against allogeneic tumor. The reaction could be augmented by the addition of recombinant interleukin-2 (rIL-2). Enzym...

journal_title：Microbiology and immunology

authors： Win S,Uenaka A,Nakayama E

更新日期：2002-01-01 00:00:00

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:An antiserum against a fraction enriched for scrapie-associated fibrils (SAF), was examined for its effects on scrapie incubation period by inoculating mice either intraperitoneally or intracerebrally with various dilutions of the serum mixed with scrapie-infected mouse brain homogenate. After intraperitoneal inoculat...

journal_title：Microbiology and immunology

authors： Ito M,Shinagawa M,Doi S,Sasaki S,Isomura H,Takahashi K,Goto H,Sato G

更新日期：1988-01-01 00:00:00

abstract：:Soil specimens obtained from several areas of Japan, which are closely located to or facing the Continental land of China, were examined for the distribution of Clostridium botulinum, especially pertaining to types A and B. A total of 266 specimens of Japan, when cultured, showed no type A or B toxicity, although 30 (...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Nishida S,Yoshimura K,Yu H,Lu DY,Nakamura S

更新日期：1988-01-01 00:00:00

abstract：:pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning...

journal_title：Microbiology and immunology

authors： Kanno R

更新日期：1988-01-01 00:00:00

abstract：:The NfxC-type mutant of Pseudomonas aeruginosa produces the MexEF-OprN efflux pump and down-regulates expression of the quorum-sensing-dependent efflux pump MexAB-OprM and production of virulence factors in the presence of an active transcriptional regulator, MexT. Consequently, these cells are resistant to chloramphe...

journal_title：Microbiology and immunology

authors： Uwate M,Ichise YK,Shirai A,Omasa T,Nakae T,Maseda H

更新日期：2013-04-01 00:00:00

abstract：:In order to count Clostridium tyrobutyricum spores in milk after membrane filtration, murine 21E7-B12 monoclonal antibody was produced. Elution of the monoclonal antibody from this antigen, the flagellar filament protein, by carbohydrate ligands was used to study the epitope structure. A competitive elution of an anti...

journal_title：Microbiology and immunology

authors： Bédouet L,Arnold F,Robreau G,Batina P,Talbot F,Malcoste R

更新日期：1998-01-01 00:00:00

abstract：:Sixty-eight sera from the acute, recurrent, and provoked types of female genital herpes were compared for the seroprevalence of herpes simplex virus (HSV) types 1 and 2 by immunodot assay using HSV glycoprotein G. In the HSV-1-isolated patients, no HSV-2 antibodies were detected, whereas in the HSV-2-isolated patients...

journal_title：Microbiology and immunology

authors： Hashido M,Lee FK,Nahmias AJ,Kawana T

更新日期：1997-01-01 00:00:00

abstract：:The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian stra...

journal_title：Microbiology and immunology

authors： Yamagata J,Ahmed K,Khawplod P,Mannen K,Xuyen DK,Loi HH,Dung NV,Nishizono A

更新日期：2007-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:The purpose of this study was to sequence the central part of the coding region of the Clostridium tyrobutyricum fiagellin gene to improve the immunoenzymatic counting of cells after milk filtration. The coding region was amplified by PCR, and the amplified products were cloned. A DNA sequence analysis of positive clo...

journal_title：Microbiology and immunology

authors： Arnold F,Bédouet L,Batina P,Robreau G

更新日期：1999-01-01 00:00:00

abstract：:The major structural proteins of Newcastle disease virus and Sendai virus were localized in infected BHK-21 and MDBK cells by ultrastructural immunoperoxidase cytochemistry using antibodies against the individual viral protein antigens. The intracellular glycoproteins were strictly membrane bound, being localized in t...

journal_title：Microbiology and immunology

authors： Nagai Y,Yoshida T,Hamaguchi M,Nagura H,Hasegawa H,Yoshimura S,Watanabe K

更新日期：1983-01-01 00:00:00

abstract：:Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an ass...

journal_title：Microbiology and immunology

authors： Kuramitsu M,Okuma K,Nakashima M,Sato T,Sasaki D,Hasegawa H,Umeki K,Kubota R,Sasada K,Sobata R,Matsumoto C,Kaneko N,Tezuka K,Matsuoka S,Utsunomiya A,Koh KR,Ogata M,Ishitsuka K,Taki M,Nosaka K,Uchimaru K,Iwanaga M

更新日期：2018-10-01 00:00:00

abstract：:To clarify the epidemiology of viral acute respiratory infections (ARIs), 305 human parainfluenza virus types 1 (HPIV1), 154 HPIV2 and 574 HPIV3 strains were isolated from 16,962 nasopharyngeal swabs obtained between 2002 and 2011 at pediatric clinics in Yamagata, Japan. The total isolation frequency for HPIV1-3 was 6...

journal_title：Microbiology and immunology

authors： Mizuta K,Abiko C,Aoki Y,Ikeda T,Itagaki T,Katsushima F,Katsushima Y,Matsuzaki Y,Noda M,Kimura H,Ahiko T

更新日期：2012-12-01 00:00:00

abstract：:Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reac...

journal_title：Microbiology and immunology

authors： Himeno K,Nomoto K,Takeya K

更新日期：1978-01-01 00:00:00

abstract：:Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...

journal_title：Microbiology and immunology

authors： Shinoda S,Kobayashi M,Yamada H,Yoshida S,Ogawa M,Mizuguchi Y

更新日期：1987-01-01 00:00:00

abstract：:The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this s...

journal_title：Microbiology and immunology

authors： Taninaka Y,Nakahara KS,Hagiwara-Komoda Y

更新日期：2020-01-01 00:00:00

abstract：:There are two types of carboxypeptidases present in human blood, carboxypeptidase N (CPN) and arginine carboxypeptidase (CPR). CPR is generated during coagulation from a precursor (proCPR) which can be converted to the active form by trypsin in vitro. Since it is difficult to distinguish the two types of carboxypeptid...

journal_title：Microbiology and immunology

authors： Guo X,Morioka A,Kaneko Y,Okada N,Obata K,Nomura T,Campbell W,Okada H

更新日期：1999-01-01 00:00:00

abstract：:Photodynamic therapy (PDT) has been considered a feasible alternative for antimicrobial therapy of multidrug-resistant pathogens. However, bacterial response mechanisms against PDT-generated photo-oxidative stress remain largely unknown. Herein, it is shown that the accessory gene regulator Agr is involved in Staphylo...

journal_title：Microbiology and immunology

authors： Park HJ,Moon YH,Yoon HE,Park YM,Yoon JH,Bang IS

更新日期：2013-08-01 00:00:00

abstract：:The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...

journal_title：Microbiology and immunology

authors： Kuwae T,Kurata M

更新日期：1984-01-01 00:00:00

abstract：:Congenitally athymic BALB/cA nu/nu mice were employed to elucidate the role of the thymus in experimental Mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. Chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. Macroscopic...

journal_title：Microbiology and immunology

authors： Tanaka H

更新日期：1979-01-01 00:00:00