New monoclonal antibodies that define multiple epitopes and a human-specific marker on the interleukin 2 receptor molecules of primates.

abstract：:Leptospira was isolated from environmental water in central Japan using selective medium comprising five antibiotics, namely sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5-fluorouracil. Of 100 water samples 57 (57%) were culture-positive and 50 pure cultures were isolated. Of the 50 cultures isolate...

journal_title：Microbiology and immunology

authors： Masuzawa T,Uno R,Matsuhashi N,Yamaguchi M,Xu J,Nakamura S

更新日期：2019-11-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5A (NS5A) is a multifunctional protein that is involved in the HCV life cycle and pathogenesis. In this study, a host protein(s) interacting with NS5A by tandem affinity purification were searched for with the aim of elucidating the role of NS5A. An NS5A-interacting prote...

journal_title：Microbiology and immunology

authors： Chen M,Gan X,Yoshino K,Kitakawa M,Shoji I,Deng L,Hotta H

更新日期：2016-06-01 00:00:00

abstract：:A number of studies have determined the contribution of Th1 and Th2 responses to the protective immunity and pathology of Mycobacterium bovis infection. However, much of that information is derived from experimentally infecting cattle with M. bovis and few data from naturally infected animals are available. The aim of...

journal_title：Microbiology and immunology

authors： Blanco FC,Schierloh P,Bianco MV,Caimi K,Meikle V,Alito AE,Cataldi AA,Sasiain Mdel C,Bigi F

更新日期：2009-08-01 00:00:00

abstract：:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...

journal_title：Microbiology and immunology

authors： Yang TH,Aosai F,Norose K,Ueda M,Yano A

更新日期：1996-01-01 00:00:00

abstract：:An extract of Candida albicans isolated from a patient with typical mucocutaneous lymph node syndrome (MCLS) can produce coronary arteritis in a mouse when injected intraperitoneally. An unusual feature of this arteritis is that it is granulomatous, shows no fibrinoid change and is confined to the coronary arteries. T...

journal_title：Microbiology and immunology

authors： Murata H

更新日期：1979-01-01 00:00:00

abstract：:One strain each of granular and downy varieties of Trichophyton mentagrophytes and two strains each of granular and floccose colonial types Trichophyton rubrum were used for preparation of antigens. The antigens were analyzed by double diffusion against homologous and heterologous antisera raised in rabbits. A total o...

journal_title：Microbiology and immunology

authors： Shukla NP,Agarwal GP

更新日期：1983-01-01 00:00:00

abstract：:The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric ...

journal_title：Microbiology and immunology

authors： Damodharan S,Gujar R,Pattabiraman S,Nesakumar M,Hanna LE,Vadakkuppattu RD,Usha R

更新日期：2013-05-01 00:00:00

abstract：:The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...

journal_title：Microbiology and immunology

authors： Toyonaga T,Matsushita O,Katayama S,Minami J,Okabe A

更新日期：1992-01-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:The extract from ECMS was investigated for its effect on the humoral immune responses to foot-and-mouth disease vaccination. Fifty-six mice were randomly divided into seven groups with eight animals in each. Mice in groups 5 to 7 were subcutaneously (s.c.) injected with 0.5 mg DEX daily for 4 days to induce immunosupp...

journal_title：Microbiology and immunology

authors： Sakwiwatkul K,Li RL,Song XM,Hu SH

更新日期：2010-01-01 00:00:00

abstract：:The production from murine resident peritoneal macrophages (M phi) of a soluble factor, which was capable of enhancing the antigen-presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M phi (M phi sup) were prepared by culturing peritoneal M phi with particles, i.e., zymosan A...

journal_title：Microbiology and immunology

authors： Miyazaki H,Ito A,Osawa T

更新日期：1988-01-01 00:00:00

abstract：:A mouse model for the study of postexposure prophylaxis of rabies was established. Mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after ...

journal_title：Microbiology and immunology

authors： Mifune K,Shichijo A,Makino Y,Takeuchi E,Yamada A,Sakamoto K

更新日期：1980-01-01 00:00:00

abstract：:The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

journal_title：Microbiology and immunology

authors： Singh JP,Verma R

更新日期：2008-08-01 00:00:00

abstract：:Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0 x 10(5) organisms p...

journal_title：Microbiology and immunology

authors： Konishi H,Morshed MG,Akitomi H,Nakazawa T

更新日期：1993-01-01 00:00:00

abstract：:The possible presence of Paul-Bunnell (PB) antigen on Epstein-Barr virus (EBV)-transformed lymphocytes were investigated. Of 23 EBV genome-positive lymphoblastoid cell lines tested all but one absorbed PB type antibody from the serum of an infectious mononucleosis patient. The one EBV-negative B cell line tested did n...

journal_title：Microbiology and immunology

authors： Maeda M,Sairenji T,Hinuma Y

更新日期：1979-01-01 00:00:00

abstract：:Frauch's micro-slide method was improved to facilitate the endpoint-determination of the Limulus test. Two precise observations, by inverted phase contrast microscopy and with a staining procedure, were newly performed as additions to the slide test. The staining procedure was proposed as an improved method for the Li...

journal_title：Microbiology and immunology

authors： Okuguchi S

更新日期：1978-01-01 00:00:00

abstract：:An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

更新日期：1985-01-01 00:00:00

abstract：:Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

journal_title：Microbiology and immunology

authors： Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

更新日期：2000-01-01 00:00:00

abstract：:L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in bo...

journal_title：Microbiology and immunology

authors： Nishikawa F,Kita E,Yamada H,Nakano A,Kashiba S

更新日期：1994-01-01 00:00:00

abstract：:A cholera-like enterotoxin was purified from Vibrio cholerae O139 strain AI-1841 isolated from a diarrheal patient in Bangladesh. Its characteristics were compared with that of cholera toxins (CTs) of classical strain 569B and El Tor strain KT25. Al-1841 produced as much toxin as O1 strains. The toxins were indistingu...

journal_title：Microbiology and immunology

authors： Nakashima K,Eguchi Y,Nakasone N

更新日期：1995-01-01 00:00:00

abstract：:EAEC is increasingly recognized as an emerging enteric pathogen. Typical EAEC expressing the AggR regulon have been proven to be an important cause of childhood diarrhea in industrialized countries as well as in the developing world, while atypical EAEC without this regulon have not been thoroughly investigated. To in...

journal_title：Microbiology and immunology

authors： Tokuda K,Nishi J,Imuta N,Fujiyama R,Kamenosono A,Manago K,Kawano Y

更新日期：2010-06-01 00:00:00

abstract：:Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and laste...

journal_title：Microbiology and immunology

authors： Kuramoto E,Toizumi S,Shimada S,Tokunaga T

更新日期：1989-01-01 00:00:00

abstract：:The killing activity of cord blood mononuclear cells (cMNC) against cytomegalovirus (CMV)-uninfected and -infected fibroblasts was comparable to that of adult peripheral blood mononuclear cells (aPBMC). The killing activity of cMNC against K562 cells was significantly lower compared with that of aPBMC. Treatment of cM...

journal_title：Microbiology and immunology

authors： Hoshina T,Kida K,Ito M

更新日期：1999-01-01 00:00:00

abstract：:Normal, thioglycollate-stimulated and BCG-activated mouse peritoneal macrophages were cultivated in vitro with the conditioned medium of mouse L-929 cells. The thioglycollate- and BCG-macrophages rapidly proliferated, whereas normal macrophages grew more slowly. A clear morphological difference between the three types...

journal_title：Microbiology and immunology

authors： Nozawa RT,Yanaki N,Yokota T

更新日期：1980-01-01 00:00:00

abstract：:β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...

journal_title：Microbiology and immunology

authors： Ishibashi K,Kurone Y,Motoi M,Miura NN,Adachi Y,Shirasu Y,Ohno N

更新日期：2010-11-01 00:00:00

abstract：:A cytotoxic T lymphocyte (CTL) response to dengue virus-infected target cells is described. Effector cells were generated in an in vitro secondary culture and appeared to be T cells possessing both the Lyt 1.1 and Lyt 2.1 surface antigens. A stronger CTL response was noted with the H-2k haplotype compared to H-2d, and...

journal_title：Microbiology and immunology

authors： Pang T,Devi S,Blanden RV,Lam SK

更新日期：1988-01-01 00:00:00

abstract：:Oral administration of adenovirus strain K87 to BALB/c nude mice resulted in viral proliferation in the intestinal tract up to around week 6 at which point replication was suppressed. In other words, the host acquired resistance. However, this resistance was temporary and the viral infection persisted over a long peri...

journal_title：Microbiology and immunology

authors： Umehara K,Hirakawa M,Hashimoto K

更新日期：1984-01-01 00:00:00

abstract：:None of the 58 Moraxella catarrhalis strains grew on nutrient agar without sodium chloride supplementation, whereas 49 of 51 commensal Neisseria spp. strains tested did. Growth on nutrient agar without sodium chloride supplementation could be used for screening between M. catarrhalis and commensal Neisseria spp. ...

journal_title：Microbiology and immunology

authors： Chida T,Sawabe E,Ono E,Nishiyama H,Ebina K,Sato K,Watanabe Y,Kuroki T,Okamura N

更新日期：2005-01-01 00:00:00

abstract：:The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...

journal_title：Microbiology and immunology

authors： Tsuboi A,Oka Y,Osaki T,Kumagai T,Tachibana I,Hayashi S,Murakami M,Nakajima H,Elisseeva OA,Fei W,Masuda T,Yasukawa M,Oji Y,Kawakami M,Hosen N,Ikegame K,Yoshihara S,Udaka K,Nakatsuka S,Aozasa K,Kawase I,Sugiyama H

更新日期：2004-01-01 00:00:00