Abstract:
:Leptospira was isolated from environmental water in central Japan using selective medium comprising five antibiotics, namely sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5-fluorouracil. Of 100 water samples 57 (57%) were culture-positive and 50 pure cultures were isolated. Of the 50 cultures isolated from water 48 were classified into a saprophytic clade on the basis of 16S ribosomal RNA gene sequences. However, it was previously reported that isolates from soil in Japan belonged to pathogenic, intermediate, and saprophytic clades, the current findings suggest less diversity of Leptospira species in environmental water than that in soil in Japan.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Masuzawa T,Uno R,Matsuhashi N,Yamaguchi M,Xu J,Nakamura Sdoi
10.1111/1348-0421.12741subject
Has Abstractpub_date
2019-11-01 00:00:00pages
469-473issue
11eissn
0385-5600issn
1348-0421journal_volume
63pub_type
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