Subcellular location of the major protein antigens of paramyxoviruses revealed by immunoperoxidase cytochemistry.

abstract：:An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

更新日期：1985-01-01 00:00:00

abstract：:Frauch's micro-slide method was improved to facilitate the endpoint-determination of the Limulus test. Two precise observations, by inverted phase contrast microscopy and with a staining procedure, were newly performed as additions to the slide test. The staining procedure was proposed as an improved method for the Li...

journal_title：Microbiology and immunology

authors： Okuguchi S

更新日期：1978-01-01 00:00:00

abstract：:The possible presence of Paul-Bunnell (PB) antigen on Epstein-Barr virus (EBV)-transformed lymphocytes were investigated. Of 23 EBV genome-positive lymphoblastoid cell lines tested all but one absorbed PB type antibody from the serum of an infectious mononucleosis patient. The one EBV-negative B cell line tested did n...

journal_title：Microbiology and immunology

authors： Maeda M,Sairenji T,Hinuma Y

更新日期：1979-01-01 00:00:00

abstract：:Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively...

journal_title：Microbiology and immunology

authors： Nakamura M,Tsutsumi K,Ooka S,Sekine T,Koizuka I,Nishioka K,Kato T

更新日期：2004-01-01 00:00:00

abstract：:Measles vaccines are highly effective and safe; however, the mechanism(s) underlying their attenuation has not been well understood. In this study, type I IFNs (IFN-α and IFN-β) induction in macaques infected with measles virus (MV) strains was examined. Type I IFNs were not induced in macaques infected with wild-type...

journal_title：Microbiology and immunology

authors： Van Nguyen N,Kato SI,Nagata K,Takeuchi K

更新日期：2016-07-01 00:00:00

abstract：:The sensitivity of influenza rapid diagnostic tests (IRDTs) currently available in Japan for various influenza virus strains, including human H7N9 and H5N1 isolates, were compared and it was found that all of the IRDTs examined detected these viruses; however, their detection sensitivities differed. ...

journal_title：Microbiology and immunology

authors： Sakai-Tagawa Y,Ozawa M,Yamada S,Uchida Y,Saito T,Takahashi K,Sugaya N,Tashiro M,Kawaoka Y

更新日期：2014-10-01 00:00:00

abstract：:Antibodies to fibronectin-binding proteins (FnBPs) of Staphylococcus aureus, including binding domain of FnBPA, the D region, or the A-C regions of FnBPB were produced in rabbits and mice. These antibodies were used to characterize cell-associated FnBPs of S. aureus strain Cowan I, S. aureus strain U320 and a coagulas...

journal_title：Microbiology and immunology

authors： Sakata N,Rozalska B,Wadström T

更新日期：1994-01-01 00:00:00

abstract：:Sixteen temperature-sensitive mutants of Sendai virus were isolated from mutagenized stocks (10 mutants, designated numerically) and persistently infected cultures (6 mutants, designated alphabetically). Based on complementation tests, virion-associated activities, thermal inactivation, and viral RNA and hemadsorbing ...

journal_title：Microbiology and immunology

authors： Adachi A,Kanda T,Shibuta H

更新日期：1980-01-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is one of the etiological agents of acute respiratory tract infections. From June 2005 to May 2006, we collected 185 clinical specimens from children in Osaka City, Japan, and detected 41 hMPV RNA. Of the 41 specimens, four (9.8%) also contained other viruses (3 with adenovirus [AdV] and 1...

journal_title：Microbiology and immunology

authors： Kaida A,Kubo H,Goto K,Shiomi M,Kohdera U,Iritani N

更新日期：2007-01-01 00:00:00

abstract：:Two methods of TSS diagnosis were evaluated: comparison of symptoms with clinical criteria and monitoring for evidence of selective activation of Vbeta2(+) T cells by the causative toxin, TSS toxin-1 (TSST-1). Ten patients with acute and systemic febrile infections caused by Staphylococcus aureus were monitored for in...

journal_title：Microbiology and immunology

authors： Matsuda Y,Kato H,Ono E,Kikuchi K,Muraoka M,Takagi K,Imanishi K,Itoh S,Itoh T,Ogawa T,Nitta K,Inokuchi S,Hibi T,Ohta H,Uchiyama T

更新日期：2008-11-01 00:00:00

abstract：:The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...

journal_title：Microbiology and immunology

authors： Toyonaga T,Matsushita O,Katayama S,Minami J,Okabe A

更新日期：1992-01-01 00:00:00

abstract：:A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

journal_title：Microbiology and immunology

authors： Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

更新日期：1987-01-01 00:00:00

abstract：:We examined the mechanisms of fluconazole resistance in a fluconazole-resistant Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis. It was demonstrated that the highly resistant phenotype of this strain was associated with combined mechanisms of the energy-dependent reduced intrace...

journal_title：Microbiology and immunology

authors： Kamai Y,Maebashi K,Kudoh M,Makimura K,Naka W,Uchida K,Yamaguchi H

更新日期：2004-01-01 00:00:00

abstract：:The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgG1 antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneou...

journal_title：Microbiology and immunology

authors： Sekiya K

更新日期：1983-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights wer...

journal_title：Microbiology and immunology

authors： Konishi E,Hotta S

更新日期：1980-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00

abstract：:Nineteen strains of Newcastle disease virus (NDV) isolated from wild ducks in Japan were placed into 4 distinct antigenic groups on the basis of their reactivities to 8 monoclonal antibodies against the HN molecule of NDV in hemagglutination inhibition tests. The NDV strains of duck origin were antigenically distinct ...

journal_title：Microbiology and immunology

authors： Kawamura M,Nagata-Matsubara K,Nerome K,Yamane N,Kida H,Kodama H,Izawa H,Mikami T

更新日期：1987-01-01 00:00:00

abstract：:The production of heat-labile enterotoxin (LT) in 76 strains of human enterotoxigenic Escherichia coli (ETEC) varied by a factor of 100. Three ETEC strains that differ in the levels of LT production were chosen for the cloning of LT genes (toxAB) into plasmid pBR322, and the gene structure and expression were compared...

journal_title：Microbiology and immunology

authors： Katayama S,Ninomiya M,Minami J,Okabe A,Hayashi H

更新日期：1990-01-01 00:00:00

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

journal_title：Microbiology and immunology

authors： Nair GB,Bhattacharya SK,Takeda T

更新日期：1993-01-01 00:00:00

abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP c...

journal_title：Microbiology and immunology

authors： Ishiguro N,Shinagawa M,Onoe S,Yamanouchi K,Saito T

更新日期：1998-01-01 00:00:00

abstract：:Clostridium perfringens KZ1340 isolated from Antarctic soil was first classified as Clostridium plagarum and later as a lecithinase-negative variant of C. perfringens. Although the strain produced no detectable lecithinase (phospholipase C, PLC) activity in the culture supernatant, it was shown by Southern blot hybrid...

journal_title：Microbiology and immunology

authors： Kameyama K,Matsushita O,Katayama S,Minami J,Maeda M,Nakamura S,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) an...

journal_title：Microbiology and immunology

authors： Shimizu T,Ohtani K,Ba-Thein W,Inui S,Nakamura S,Hayashi H

更新日期：1996-01-01 00:00:00

abstract：:A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 μg/mL; amphotericin B, 5 μg/mL; fosfomycin, 400 μg/mL; and 5-fluorouracil, 100 μg/mL) was developed for selective isolation of leptospires from contaminated samples. The growth of 16 microorganisms considered as possible contamin...

journal_title：Microbiology and immunology

authors： Chakraborty A,Miyahara S,Villanueva SY,Saito M,Gloriani NG,Yoshida S

更新日期：2011-07-01 00:00:00

abstract：:We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in ...

journal_title：Microbiology and immunology

authors： Saito M,Kajiwara H,Miyamoto H,Yoshida S

更新日期：2001-01-01 00:00:00

abstract：:Lancefield group C Streptococcus dysgalactiae causes infections in farmed fish. Here, the genome of S. dysgalactiae strain kdys0611, isolated from farmed amberjack (Seriola dumerili) was sequenced. The complete genome sequence of kdys0611 consists of a single chromosome and five plasmids. The chromosome is 2,142,780 b...

journal_title：Microbiology and immunology

authors： Nishiki I,Yoshida T,Fujiwara A

更新日期：2019-07-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5B (NS5B) is an RNA replicase. We expressed full-length NS5B (591 amino acid residues) in Escherichia coli as a fusion protein with maltose binding protein (MBP-NS5B). MBP-NS5B was recovered in the soluble fraction after centrifugation at 40,000 x g and affinity-purified ...

journal_title：Microbiology and immunology

authors： Tanaka T,Sugiyama K,Ikeda M,Naganuma A,Nozaki A,Saito M,Shimotohno K,Kato N

更新日期：2000-01-01 00:00:00

abstract：:Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two-dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg-gingipain (Rgp) as determined by N-terminal amino acid s...

journal_title：Microbiology and immunology

authors： Kamaguchi A,Nakano M,Shoji M,Nakamura R,Sagane Y,Okamoto M,Watanabe T,Ohyama T,Ohta M,Nakayama K

更新日期：2004-01-01 00:00:00