Facilitated production of secretory IgA against Shiga toxin B subunits by intranasal application of antigen-coated polystyrene microspheres.

Abstract:

:We examined the effects of microspheres as antigen carriers in mucosal immunization. Shiga toxin B subunits (Stx1B) were adsorbed on 6 mum polystyrene microspheres, which were then intranasally administered to mice together with cholera toxin (CT). Stx1B-specific serum IgG production and secretory IgA production at local mucosal sites were enhanced by the use of microspheres. When OVA was used as a model antigen, secretory IgA production but not serum IgG production was enhanced on the use of microspheres. These results indicated that microspheres provide a useful means of potentiating the immune response against Stx1B with weak immunogenicity.

journal_name

Microbiol Immunol

authors

Kurohane K,Kobayashi C,Imai Y

doi

10.1111/j.1348-0421.2005.tb03714.x

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

149-54

issue

2

eissn

0385-5600

issn

1348-0421

pii

JST.JSTAGE/mandi/49.149

journal_volume

49

pub_type

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