Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infection in vitro.

abstract：:A mouse model for the study of postexposure prophylaxis of rabies was established. Mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after ...

journal_title：Microbiology and immunology

authors： Mifune K,Shichijo A,Makino Y,Takeuchi E,Yamada A,Sakamoto K

更新日期：1980-01-01 00:00:00

abstract：:Because cytotoxic T lymphocytes (CTLs) play an important role in the specific immunotherapy of hepatitis C virus (HCV) infection, a series of CTL epitopes has been defined from HCV genotype 1a or 1b protein. Here, we attempted to identify HCV2a-derived epitopes that are capable of inducing HLA-A2-restricted and peptid...

journal_title：Microbiology and immunology

authors： Wang Y,Takao Y,Harada M,Yutani S,Ide T,Sata M,Itoh K,Yamada A

更新日期：2006-01-01 00:00:00

abstract：:Mice primed with 1 microgram of reduced and alkylated ovalbumin (RA-OA) developed not only long-lived memory cells for delayed-type hypersensitivity (DTH), capable of differentiating into DTH-effector T cells (DTH-Te) against ovalbumin (OA) when restimulated in vitro with OA, but also spleen cells capable of augmentin...

journal_title：Microbiology and immunology

authors： Kojima A,Tamura SI,Egashira Y

更新日期：1982-01-01 00:00:00

abstract：:Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits,...

journal_title：Microbiology and immunology

authors： Nishioka M,Aibiki T,Shirai M,Terada S,Kagawa H,Watanabe S

更新日期：1986-01-01 00:00:00

abstract：:Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two-dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg-gingipain (Rgp) as determined by N-terminal amino acid s...

journal_title：Microbiology and immunology

authors： Kamaguchi A,Nakano M,Shoji M,Nakamura R,Sagane Y,Okamoto M,Watanabe T,Ohyama T,Ohta M,Nakayama K

更新日期：2004-01-01 00:00:00

abstract：:BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA spec...

journal_title：Microbiology and immunology

authors： He F,Morita H,Kubota A,Ouwehand AC,Hosoda M,Hiramatsu M,Kurisaki J

更新日期：2005-01-01 00:00:00

abstract：:B. pertussis is a causative agent of whooping cough (pertussis) in humans. Despite wide-scale vaccination in many countries, there is serious concern about pertussis as a re-emerging disease. Re-emergence of pertussis may be explained by several factors: the short duration of protection by the currently available acel...

journal_title：Microbiology and immunology

authors： Abe A,Nagamatsu K,Watanabe M

更新日期：2008-02-01 00:00:00

abstract：:A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

journal_title：Microbiology and immunology

authors： Nakatani T,Higuchi A,Noguchi H

更新日期：1992-01-01 00:00:00

abstract：:Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on sixteen human isolates of serotype 2 of rotavirus in Japan, China, and Pakistan and their genetic variations were examined. Comparative studies of their nucleotide and deduced amino acid sequences between the sixteen is...

journal_title：Microbiology and immunology

authors： Wen L,Ushijima H,Kakizawa J,Fang ZY,Nishio O,Morikawa S,Motohiro T

更新日期：1995-01-01 00:00:00

abstract：:We observed the effects of a chinese herb medicine Sho-saiko-to on the lethal and antitumor activities of recombinant human tumor necrosis factor (rhTNF) administered in mice. Sho-saiko-to was noted to protect the rhTNF-induced lethality in galactosamine-hypersensitized mice, and also Sho-saiko-to pretreated mice was ...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Tutumi E,Yokota K,Furusawa S,Sasaki K,Takayanagi Y

更新日期：1991-01-01 00:00:00

abstract：:Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of ...

journal_title：Microbiology and immunology

authors： Wang R,Chen S,Jin J,Ren F,Li Y,Qiao Z,Wang Y,Zhao L

更新日期：2015-05-01 00:00:00

abstract：:The entire cloned human adenovirus type 5 (Ad5) genome is known to be able to generate infectious virus after transfection into 293 cells when the both ends of the genome are exposed by digestion with appropriate restriction enzymes. However, when one or both ends of the genome are tagged with nucleotides and are not ...

journal_title：Microbiology and immunology

authors： Fukuda H,Terashima M,Koshikawa M,Kanegae Y,Saito I

更新日期：2006-01-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:Measles vaccines are highly effective and safe; however, the mechanism(s) underlying their attenuation has not been well understood. In this study, type I IFNs (IFN-α and IFN-β) induction in macaques infected with measles virus (MV) strains was examined. Type I IFNs were not induced in macaques infected with wild-type...

journal_title：Microbiology and immunology

authors： Van Nguyen N,Kato SI,Nagata K,Takeuchi K

更新日期：2016-07-01 00:00:00

abstract：:Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

journal_title：Microbiology and immunology

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2001-01-01 00:00:00

abstract：:The protective role of neutrophils on intranasal infection of influenza virus was investigated in 3 strains of tumor-bearing mice with neutrophilic leukocytosis. In vitro multiplication of influenza virus was inhibited by neutrophils from both normal and tumor-bearing mice, and the inhibitory effect of neutrophils was...

journal_title：Microbiology and immunology

authors： Fujisawa H

更新日期：2001-01-01 00:00:00

abstract：:Bacterial images can be obtained rather easily with an atomic-force microscope (AFM) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. The bacterial shapes or the presence of flagella can be clearly recogni...

journal_title：Microbiology and immunology

authors： Umeda A,Saito M,Amako K

更新日期：1998-01-01 00:00:00

abstract：:Leptospirosis is a worldwide zoonosis. The importance of urban leptospirosis is recognized in Japan: urban rats carry pathogenic leptospires and people acquire these pathogens through contact with surface water or soil contaminated by the urine of the infected animals. To determine the current Leptospira carriage rate...

journal_title：Microbiology and immunology

authors： Saito M,Villanueva SY,Masuzawa T,Haraguchi Y,Ita S,Miyahara S,Ozuru R,Yamaguchi T,Yoshimura M,Ikejiri M,Aramaki N,Amran MY,Muslich LT,Iida K,Yanagihara Y,Gloriani NG,Yoshida S

更新日期：2015-06-01 00:00:00

abstract：:Interleukin 8 (IL-8) is induced in many cell types by various stimuli including virus infection. It was reported that nonstructural protein 5A (NS5A) of hepatitis C virus (HCV) was involved in induction of IL-8 expression at both mRNA and protein levels in cultured human cells. In this study, we aimed to determine whe...

journal_title：Microbiology and immunology

authors： Kadoya H,Nagano-Fujii M,Deng L,Nakazono N,Hotta H

更新日期：2005-01-01 00:00:00

abstract：:Cell-mediated immune response to Japanese encephalitis virus (JEV) and its purified envelope (E) protein was measured in 45 laboratory confirmed JE patients using a proliferation assay of peripheral blood mononuclear cells (PBMC). In parallel, JEV-specific IgM antibodies were measured by ELISA. No correlation was obse...

journal_title：Microbiology and immunology

authors： Desai A,Ravi V,Chandramuki A,Gourie-Devi M

更新日期：1995-01-01 00:00:00

abstract：:Aged C57BL/6 (B6) mice could reject allogeneic BALB/c RL male 1 tumor as efficiently as young B6 mice. However, in vitro analysis showed impaired generation of cytotoxic T cell response in aged B6 mice against allogeneic tumor. The reaction could be augmented by the addition of recombinant interleukin-2 (rIL-2). Enzym...

journal_title：Microbiology and immunology

authors： Win S,Uenaka A,Nakayama E

更新日期：2002-01-01 00:00:00

abstract：:We have previously reported a mouse model of severe group A streptococcal infection (Microbiol. Immunol. 45: 777-786, 2001). When we injected Streptococcus pyogenes strains intramuscularly, the mice suffered from acute phase of infection for a few days but recovered from the illness and gained body weight. These mice,...

journal_title：Microbiology and immunology

authors： Iida K,Seki M,Saito M,Kawamura Y,Kajiwara H,Yoshida S

更新日期：2006-01-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:Quantitative PCR is becoming widespread for diagnosing and monitoring post-transplantation diseases associated with EBV and CMV. These assays need to be standardized to manage patients in different facilities. Five independent laboratories in Japan compared home-brew assays and a prototype assay system to establish a ...

journal_title：Microbiology and immunology

authors： Ito Y,Takakura S,Ichiyama S,Ueda M,Ando Y,Matsuda K,Hidaka E,Nakatani K,Nishioka J,Nobori T,Kajiyama N,Kimura H

更新日期：2010-09-01 00:00:00

abstract：:Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expre...

journal_title：Microbiology and immunology

authors： Na HN,Kim KH,Song MK,Park HL,Lee EY,Shim SH,Park S,Nam JH

更新日期：2013-09-01 00:00:00

abstract：:A polymyxin B (PXB)-resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB-resistant isolate bound little PXB, although LPS from the parental strain did. The 31P nuclear magnetic resonance (NMR) spectrum of PXB-resistant type LPS showed that it contained much less of...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Jiang GZ,Kato Y,Sugiyama T,Kawai M,Fukada M,Takahashi K

更新日期：1994-01-01 00:00:00

abstract：:In Streptococcus pneumoniae, heat shock induces the synthesis of 65-, 73-, and 84-kDa proteins, and ethanol shock induces a 104-kDa protein. In this study, the 65-, 84-, and 104-kDa proteins were identified as members of the GroEL, ClpL and alcohol dehydrogenase families, respectively, and the general properties of th...

journal_title：Microbiology and immunology

authors： Choi IH,Shim JH,Kim SW,Kim SN,Pyo SN,Rhee DK

更新日期：1999-01-01 00:00:00

abstract：:Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of...

journal_title：Microbiology and immunology

authors： Hotsubo T,Nagata N,Shimada M,Yoshida K,Fujinaga K,Chiba S

更新日期：1994-01-01 00:00:00

abstract：:The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this s...

journal_title：Microbiology and immunology

authors： Taninaka Y,Nakahara KS,Hagiwara-Komoda Y

更新日期：2020-01-01 00:00:00

abstract：:To evaluate a modified rapid ELISA method for detecting CAg during Toxoplasma gondii infection, we analyzed the specificity and sensitivity of the ELISA method by using experimental Toxoplasma infection in rabbits and also tested this method in human samples including 5428 serum, 548 cerebrospinal fluid and two breast...

journal_title：Microbiology and immunology

authors： Chen R,Lu S,Lou D,Lin A,Zeng X,Ding Z,Wen L,Ohta N,Wang J,Fu C

更新日期：2008-03-01 00:00:00