Multicenter evaluation of prototype real-time PCR assays for Epstein-Barr virus and cytomegalovirus DNA in whole blood samples from transplant recipients.

abstract：:The concept of superantigens is well-known and widely accepted. In this brief communication, we analyze the behaviour of antigen-presenting cells after T-cell activation by staphylococcal enterotoxin B, a representative superantigen. We tried to activate murine T cells by inflammatory mouse peritoneal macrophage in th...

journal_title：Microbiology and immunology

authors： Sakurada J,Tajima A,Shinji H,Seki K,Masuda S

更新日期：2000-01-01 00:00:00

abstract：:The fusion of BHK-21-KB cells by vesicular stomatitis virus was not induced in Eagle's minimal essential medium without glucose. In medium containing glucose, the rate of polykaryocyte formation decreased as the concentration of glucose was reduced below 5 mM. However, no reduction in virus production 24 hr after infe...

journal_title：Microbiology and immunology

authors： Takehara M

更新日期：1981-01-01 00:00:00

abstract：:During the three-year period from 1984 to 1987, 506 acute gastroenteritis outbreaks involving 14,383 patients were reported to the Bureau of Public Health, Tokyo Metropolitan Government. Eighty (4,324 patients) of 150 outbreaks (4,860 patients) from which etiologic agents were not identified were subjected to virologi...

journal_title：Microbiology and immunology

authors： Sekine S,Okada S,Hayashi Y,Ando T,Terayama T,Yabuuchi K,Miki T,Ohashi M

更新日期：1989-01-01 00:00:00

abstract：:An environmental isolate of V. mimicus, strain E-33, has been reported to produce and secrete a hemolysin of 63 kDa. The hemolysin is enterotoxic in test animals. The nucleotide sequence of the structural gene of the hemolysin was determined. We found a 2,232 bp open reading frame, which codes a peptide of 744 amino a...

journal_title：Microbiology and immunology

authors： Rahman MM,Miyoshi S,Tomochika K,Wakae H,Shinoda S

更新日期：1997-01-01 00:00:00

abstract：:The fermentation-inhibition (FI) test for Mycoplasma pneumoniae was improved by using a combination of guinea pig complement and gamma globulin-depleted horse serum in place of unheated whole horse serum employed in the conventional assay system. As the test antigen for the new FI assay system, M. pneumoniae filtrated...

journal_title：Microbiology and immunology

authors： Okazaki N,Akema R,Takizawa K

更新日期：1991-01-01 00:00:00

abstract：:To determine the extent of genetic diversity of Salmonella choleraesuis subspecies choleraesuis serovar Infantis and whether environmental isolates were similar or identical to human isolates, a total of 110 isolates from humans, broiler samples, egg production facilities, riverwater, sewage, and chicken meat were ana...

journal_title：Microbiology and immunology

authors： Murakami K,Horikawa K,Otsuki K

更新日期：1999-01-01 00:00:00

abstract：:The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase ac...

journal_title：Microbiology and immunology

authors： Igarashi K,Sasada R,Niiyama Y,Kozai Y,Sugino Y

更新日期：1979-01-01 00:00:00

abstract：:Autoantibodies to myeloperoxidase (MPO) are a subset of anti-neutrophil cytoplasmic antibody (ANCA, MPO-ANCA) detected in the sera of some patients with primary systemic vasculitis. The titer of MPO-ANCA does not always reflect disease activity and this inconsistency may be attributable to differences in epitopic spec...

journal_title：Microbiology and immunology

authors： Suzuki K,Kobayashi S,Yamazaki K,Gondo M,Tomizawa K,Arimura Y,Nakabayashi K,Ozaki S,Yoshida M,Yoshida T,Tsusaka N,Muso E,Okazaki T,Hashimoto H

更新日期：2007-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00

abstract：:Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of...

journal_title：Microbiology and immunology

authors： Hotsubo T,Nagata N,Shimada M,Yoshida K,Fujinaga K,Chiba S

更新日期：1994-01-01 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00

abstract：:Previously, we have reported the occurrence of novel phosphocholine-containing glycoglycerolipids (GGPLs: GGPL-I and GGPL-III) in human helper T-cell (MT-4 cell line) (Mustuda et al, Glycoconjugate J. 10:340). However, the GGPLs disappeared from the MT-4 after treatment with an antimycoplasma agent. This disappearance...

journal_title：Microbiology and immunology

authors： Matsuda K,Harasawa R,Li JL,Kasama T,Taki T,Handa S,Yamamoto N

更新日期：1995-01-01 00:00:00

abstract：:To establish an in vitro method of predicting in vivo efficacy of antifungal drugs against Candida albicans and Aspergillus fumigatus, the antifungal activities of fluconazole, itraconazole, and amphotericin B were determined in mouse serum. The minimum inhibitory concentration (MIC) of each drug was measured using mo...

journal_title：Microbiology and immunology

authors： Maki K,Watabe E,Iguchi Y,Nakamura H,Tomishima M,Ohki H,Yamada A,Matsumoto S,Ikeda F,Tawara S,Mutoh S

更新日期：2006-01-01 00:00:00

abstract：:No systematic study on serotyping of Enterococcus faecalis has been reported since 1964 when M.E. Sharpe conducted serotyping of group D streptococcus in U.K. So, we attempted to re-evaluate serotyping of E. faecalis. For this purpose, we received 42 Sharpe's strains and first examined for their biochemical characteri...

journal_title：Microbiology and immunology

authors： Maekawa S,Yoshioka M,Kumamoto Y

更新日期：1992-01-01 00:00:00

abstract：:The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgG1 antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneou...

journal_title：Microbiology and immunology

authors： Sekiya K

更新日期：1983-01-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for detecting Clostridium difficile toxin A is presented. Purified monoclonal antibody (mAb 37B5) was used for latex sensitization. The culture supernatants of 93 strains of C. difficile were tested by RPLA assay and the results compared with those of a commercially ...

journal_title：Microbiology and immunology

authors： Toma C,Nakamura S,Kamiya S,Nakasone N,Iwanaga M

更新日期：1999-01-01 00:00:00

abstract：:An important toxin-antitoxin (TA) system hok/sok, encoded by R1 plasmid of Escherichia coli, is involved in the post segregation killing of cells that have lost the plasmid. The lethal properties of hok protein have been utilized for the environmental containment of microbes and the development of potential vaccine ca...

journal_title：Microbiology and immunology

authors： Kaur A,Muthukumarappa T,Kanta P,Banday AZ,Chidananda MK

更新日期：2020-11-01 00:00:00

abstract：:Periapical lesions are induced by bacterial infection of the dental pulp and result in destruction of the surrounding alveolar bone. Although various immunological studies concerning periapical bone resorption have been reported, the role of cytokines in the formation of periapical lesions remains unclear. In this stu...

journal_title：Microbiology and immunology

authors： Oseko F,Yamamoto T,Akamatsu Y,Kanamura N,Iwakura Y,Imanishi J,Kita M

更新日期：2009-05-01 00:00:00

abstract：:In order to elucidate the molecular characteristics of Japanese encephalitis (JE) virus in Okinawa, 23 strains of JE virus isolated in a 25-year span were sequenced for the 240 nucleotides of the C-preM junction region and 111 nucleotides of the E gene region and compared with those of reference strains isolated in ma...

journal_title：Microbiology and immunology

authors： Ma SP,Arakaki S,Makino Y,Fukunaga T

更新日期：1996-01-01 00:00:00

abstract：:In recent years, phosphatidylinositol 4-kinase III beta (PI4KB) has emerged as a conserved target of anti-picornavirus compounds. In the present study, PI4KB was identified as the direct target of the plant-derived anti-picornavirus compounds, oxoglaucine and pachypodol (also known as Ro 09-0179). PI4KB was also ident...

journal_title：Microbiology and immunology

authors： Arita M,Philipov S,Galabov AS

更新日期：2015-06-01 00:00:00

abstract：:Effects of each of three indigenous Lactobacillus groups on other bacterial populations were separately investigated in gnotobiotic rats. In the wall of the non-glandular part of the stomach, contents of the stomach and contents of the upper part of the small intestine, some pre-associated indigenous bacteria were red...

journal_title：Microbiology and immunology

authors： Watanabe T,Morotomi M,Kawai Y,Mutai M

更新日期：1977-01-01 00:00:00

abstract：:Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...

journal_title：Microbiology and immunology

authors： Machida K,Sakurai S,Kondo I,Ikawa S

更新日期：1988-01-01 00:00:00

abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI) is an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). TAFI in plasma is presumed to influence vascular disease in its role as a fibrinol...

journal_title：Microbiology and immunology

authors： Akatsu H,Ishiguro M,Ogawa N,Kanesaka T,Okada N,Yamamoto T,Campbell W,Okada H

更新日期：2007-01-01 00:00:00

abstract：:The purpose of this study was to clarify the taxonomic relationship between all the serovars and species of the genus Erysipelothrix by performing DNA-DNA hybridization experiments, the customary criterion for separation of bacterial genospecies. A total of 93 strains were isolated from a wide variety of sources, incl...

journal_title：Microbiology and immunology

authors： Takahashi T,Fujisawa T,Umeno A,Kozasa T,Yamamoto K,Sawada T

更新日期：2008-10-01 00:00:00

abstract：:The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of ...

journal_title：Microbiology and immunology

authors： Iizawa Y,Nakao M,Kondo M,Yamazaki T

更新日期：1990-01-01 00:00:00

abstract：:Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...

journal_title：Microbiology and immunology

authors： Wada K,Nagai H,Hagiwara T,Ibe S,Utsumi M,Kaneda T

更新日期：2004-01-01 00:00:00

abstract：:Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

journal_title：Microbiology and immunology

authors： Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

更新日期：1996-01-01 00:00:00

abstract：:Shigella sonnei, which has generally been associated with dysentery in developed countries, has recently been emerging in developing countries. Specifically, in Brazil few published studies have that molecularly characterized this species. The aims of this study were to analyze the efficacy of typing using multiple-lo...

journal_title：Microbiology and immunology

authors： Seribelli AA,Frazão MR,Medeiros MIC,Stehling EG,Falcão JP

更新日期：2017-12-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00

abstract：:A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine af...

journal_title：Microbiology and immunology

authors： Guo X,Jia H,Zhang Q,Yuan W,Zhu G,Xin T,Zhu H,Sánchez-Vizcaíno JM

更新日期：2012-05-01 00:00:00