Determination of antifungal activities in serum samples from mice treated with different antifungal drugs allows detection of an active metabolite of itraconazole.

abstract：:We previously reported a helper T-cell (Th) epitope (peptide i) which corresponded to the sequence ranging from positions 462 to 479 from the N-terminus of the Friend-murine leukemia virus (F-MuLV) envelope protein (env462-479). Homologous sequences exist in both Moloney-murine leukemia (M-MuLV env452-469) and endogen...

journal_title：Microbiology and immunology

authors： Uenishi H,Iwanami N,Yamagishi H,Nakatani T,Kawasaki T,Tamamura H,Fujii N,Kuribayashi K

更新日期：1998-01-01 00:00:00

abstract：:Investigation of 136 turkeys (24 manifesting infra-orbital sinusitis, 112 apparently healthy) resulted in isolation of 79 strains of Mycoplasma and 4 of Acholeplasma. By the disc growth inhibition test with 16 reference antisera of avian serogroups, 55 strains were identified serologically and 28 remained unidentified...

journal_title：Microbiology and immunology

authors： El Sayed SA,Chandiramani NK,Garg DN

更新日期：1981-01-01 00:00:00

abstract：:We have developed a convenient method for assaying the receptor-destroying enzyme (RDE) activity of influenza C virus. This method measures the ability of the RDE to destroy the hemagglutination-inhibition activity of a potent inhibitor present in rat serum. Some physico-chemical properties of the RDE of influenza C v...

journal_title：Microbiology and immunology

authors： Sugawara K,Kitame F,Homma M,Nakamura K

更新日期：1985-01-01 00:00:00

abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:Site-specific recombinase is widely applied for the regulation of gene expression because its regulatory action is strict and efficient. However, each system can mediate regulation of only one gene at a time. Here, we demonstrate efficient "sequential" gene regulation using Cre-and FLP-expressing recombinant adenoviru...

journal_title：Microbiology and immunology

authors： Kondo S,Takahashi Y,Shiozawa S,Ichise H,Yoshida N,Kanegae Y,Saito I

更新日期：2006-01-01 00:00:00

abstract：:The function of MHC class II HLA-DR molecules expressed on a human B lymphoid progenitor cell line FL8.2.4.4 (abbreviated as FL4.4) was examined. FL4.4 cells expressed HLA-DR molecules and stimulation of the DR molecules by anti-DR mAb or by superantigen TSST-1 induced strong augmentation of homocytic aggregation and ...

journal_title：Microbiology and immunology

authors： Naitoh K,Ichigi Y,Miyake K,Muraguchi A,Kimoto M

更新日期：1994-01-01 00:00:00

abstract：:Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tub...

journal_title：Microbiology and immunology

authors： Osada-Oka M,Tateishi Y,Hirayama Y,Ozeki Y,Niki M,Kitada S,Maekura R,Tsujimura K,Koide Y,Ohara N,Yamamoto T,Kobayashi K,Matsumoto S

更新日期：2013-01-01 00:00:00

abstract：:Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Iwata H,Miyadai T,Kimura Y

更新日期：1988-01-01 00:00:00

abstract：:In malignant B lymphoma cells interleukin-10 (IL-10) expression is frequently upregulated. This effect is thought to support to the malignant transformation of these cells and to be a potential target for pharmacotherapy. To define better the mechanism for upregulation of the IL-10 gene, we tested the association betw...

journal_title：Microbiology and immunology

authors： Horie K,Ohashi M,Satoh Y,Sairenji T

更新日期：2007-01-01 00:00:00

abstract：:A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

journal_title：Microbiology and immunology

authors： Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

更新日期：1999-01-01 00:00:00

abstract：:Four T antigen-positive phenotypic revertants were isolated by negative selection with BUdR from SV40-transformed rat and mouse cells which contain six and two viral genome equivalents per cell, respectively. Karyological analysis indicated that one rat and one mouse revertant had a hyperploid number of chromosomes, w...

journal_title：Microbiology and immunology

authors： Maruyama K,Furuta T,Ueda K,Oda K

更新日期：1982-01-01 00:00:00

abstract：:Congenitally athymic BALB/cA nu/nu mice were employed to elucidate the role of the thymus in experimental Mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. Chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. Macroscopic...

journal_title：Microbiology and immunology

authors： Tanaka H

更新日期：1979-01-01 00:00:00

abstract：:The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric ...

journal_title：Microbiology and immunology

authors： Damodharan S,Gujar R,Pattabiraman S,Nesakumar M,Hanna LE,Vadakkuppattu RD,Usha R

更新日期：2013-05-01 00:00:00

abstract：:Butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii was cultured with peripheral blood mononuclear cells (PBM) in the presence of sub- and/or supra-optimal mitogenic concentrations of concanavalin A (Con A). The addition of Bu-WSA resulted in increased tritiated thymidine incorpora...

journal_title：Microbiology and immunology

authors： Nitta T,Okumura S,Tsushi M,Nakano M

更新日期：1982-01-01 00:00:00

abstract：:Peptone-yeast extract (PY) medium containing 0.035% ferric ammonium citrate as an indicator, 0.05% sulfite as a substrate, 0.05% cysteine as a reducer and 0.5% glucose was found to be suitable for observing the sulfite reduction test. The effect of added cysteine on the test was suppressed by the addition of glucose. ...

journal_title：Microbiology and immunology

authors： Kawabata N

更新日期：1980-01-01 00:00:00

abstract：:A new type of immuno-cell therapy called BRM-activated killer (BAK) therapy using non-MHC-restricted lymphocytes, CD56-positive cells, was devised. Peripheral blood lymphocytes were selected by immobilization with anti-CD3 monoclonal antibody and cultured for 2 weeks in the presence of IL-2. Thereafter, they were reac...

journal_title：Microbiology and immunology

authors： Ebina T,Ogama N,Shimanuki H,Kubota T,Isono N

更新日期：2001-01-01 00:00:00

abstract：:A solubilized sheep red blood cell (SRBC) antigen (supernatant fraction obtained by centrifuging 10(7)-2 x 108 sonicated SRBC at 6 x 10(4) g for 30 min [Sup-SRBC]), whose ability to inhibit anti-SRBC plaque formation was 70% of that of the original sonicated SRBC, was unable to elicit a detectable antibody response in...

journal_title：Microbiology and immunology

authors： Nagase F,Nakashima I,Nagase N,Kato N

更新日期：1982-01-01 00:00:00

abstract：:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhim...

journal_title：Microbiology and immunology

authors： Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S

更新日期：1995-01-01 00:00:00

abstract：:Both cluster of differentiation (CD)4(+) and CD8(+) T lymphocytes play key roles in immunity to Brucella, in part because they secrete interferon (IFN)-γ and activate bactericidal functions in macrophages. Therefore, use of markers of macrophage activation may have diagnostic and prognostic significance. High-mobili...

journal_title：Microbiology and immunology

authors： Ayarcı AO,Yılmaz E,Sığırlı D,Budak F,Göral G,Oral HB

更新日期：2013-02-01 00:00:00

abstract：:The colH gene encoding 116-kDa collagenase of Clostridium histolyticum (cColH) was cloned into an Escherichia coli-Bacillus subtilis shuttle vector to develop a method for purification of recombinant collagenase (rColH). When plasmid pJCM310 containing the colH gene was introduced into B. subtilis DB104 and the transf...

journal_title：Microbiology and immunology

authors： Jung CM,Matsushita O,Katayama S,Minami J,Ohhira I,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:Live attenuated bacteria can be used as a carrier for the delivery of foreign antigens to a host's immune system. The N-terminal domain of SipB, a translocon protein of the type III secretion system of Salmonella enterica serovar Typhimurium, is required for secretion and outer membrane localization. In the present st...

journal_title：Microbiology and immunology

authors： Jang JI,Kim JS,Eom JS,Kim HG,Kim BH,Lim S,Bang IS,Park YK

更新日期：2012-09-01 00:00:00

abstract：:Normal, thioglycollate-stimulated and BCG-activated mouse peritoneal macrophages were cultivated in vitro with the conditioned medium of mouse L-929 cells. The thioglycollate- and BCG-macrophages rapidly proliferated, whereas normal macrophages grew more slowly. A clear morphological difference between the three types...

journal_title：Microbiology and immunology

authors： Nozawa RT,Yanaki N,Yokota T

更新日期：1980-01-01 00:00:00

abstract：:The fermentation-inhibition (FI) test for Mycoplasma pneumoniae was improved by using a combination of guinea pig complement and gamma globulin-depleted horse serum in place of unheated whole horse serum employed in the conventional assay system. As the test antigen for the new FI assay system, M. pneumoniae filtrated...

journal_title：Microbiology and immunology

authors： Okazaki N,Akema R,Takizawa K

更新日期：1991-01-01 00:00:00

abstract：:The activity of glucose dehydrogenase present in resting spores of Bacillus subtilis varied strikingly with the conditions for disrupting the spores by sonic treatment, namely, the time and strength of sonication, and the type and pH of the solution used for suspending the spores. When the resting spores were sonicate...

journal_title：Microbiology and immunology

authors： Tochikubo K,Yasuda Y

更新日期：1983-01-01 00:00:00

abstract：:No systematic study on serotyping of Enterococcus faecalis has been reported since 1964 when M.E. Sharpe conducted serotyping of group D streptococcus in U.K. So, we attempted to re-evaluate serotyping of E. faecalis. For this purpose, we received 42 Sharpe's strains and first examined for their biochemical characteri...

journal_title：Microbiology and immunology

authors： Maekawa S,Yoshioka M,Kumamoto Y

更新日期：1992-01-01 00:00:00

abstract：:Fibronectin is known to be an important factor in colonization by Staphylococcus aureus of host tissues as well as other extracellular matrix proteins such as collagen and laminin. We investigated the effect of fibronectin on the phagocytosis of the S. aureus Cowan I strain by macrophages and of coagulase-negative sta...

journal_title：Microbiology and immunology

authors： Shinji H,Sakurada J,Seki K,Murai M,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:The effects of added maltose on the activities of a preparation of crude glucosyltransferases (GTases) and purified dextransucrase (DS) were investigated to elucidate the inhibition mechanism of maltose on the synthesis of water-insoluble glucan (ISG) in Streptococcus mutans HS-6. Tri- and tetra-saccharides produced b...

journal_title：Microbiology and immunology

authors： Fukui K,Moriyama T

更新日期：1983-01-01 00:00:00

abstract：:A bacterial strain with affinity to Candida albicans was successfully obtained from a natural environment. An uncovered Petri dish containing a suspension of heat-killed C. albicans cells was allowed to stand in a laboratory for several days. Some bacteria which had adhered to the candidal cells were tested for their ...

journal_title：Microbiology and immunology

authors： Seki K,Kobayashi K,Tomiya Y,Sakurada J,Murai M,Usui A,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences fla...

journal_title：Microbiology and immunology

authors： Tabuchi A

更新日期：1985-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00