Spontaneous production of a C-type RNA virus in a cell line derived from rat glioma.

abstract：:The gH of CMV is a major target for strain-specific neutralizing antibodies. To verify whether there is a correlation between HLA-DR type and strain-specific antibodies, antibodies against CMV gH in potential donors and recipients for renal transplantation were investigated. Among 471 subjects, 404 (86%) showed reacti...

journal_title：Microbiology and immunology

authors： Ishibashi K,Tokumoto T,Shirakawa H,Hashimoto K,Kushida N,Yanagida T,Shishido K,Aikawa K,Yamaguchi O,Toma H,Tanabe K,Suzutani T

更新日期：2009-07-01 00:00:00

abstract：:A sensitive, quantitative, short-time, and reproducible focus assay for Japanese encephalitis (JE) virus is described. After 2 or 3 days of incubation, the infected cells were treated with anti-JE virus serum and complement, and subsequently stained with trypan blue; then clear foci were produced. This method made it ...

journal_title：Microbiology and immunology

authors： Kimura-Kuroda J,Yasui K

更新日期：1985-01-01 00:00:00

abstract：:When the R-form lipopolysaccharide (LPS) from Klebsiella pneumoniae strain LEN-111 (O3-:K1-), from which cationic material had been removed by electrodialysis, was suspended in 50 mM Tris buffer at pH 8.5 containing 0.1 mM or higher concentrations of MgCl2, it formed an ordered two-dimensional hexagonal lattice struct...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Arakawa Y,Ito H,Naito S

更新日期：1990-01-01 00:00:00

abstract：:DNase B is a major nuclease and a possible virulence factor in Streptococcus pyogenes. The allelic diversity of streptococcal DNase B (sdaB) gene was investigated in 83 strains with 14 emm genotypes. Of the 15 alleles identified, 11 alleles carried only synonymous nucleotide substitutions. On the other hand, 4 alleles...

journal_title：Microbiology and immunology

authors： Matsumoto M,Sakae K,Hashikawa S,Torii K,Hasegawa T,Horii T,Endo M,Okuno R,Murayama S,Hirasawa K,Suzuki R,Isobe J,Tanaka D,Katsukawa C,Tamaru A,Tomita M,Ogata K,Ikebe T,Watanabe H,Ohta M,Working Group for Group A S

更新日期：2005-01-01 00:00:00

abstract：:We studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. Escherichia coli, Klebsiella pneumoniae, serratia marcescens, Pseudomonas aeruginosa, and Acinetobacte...

journal_title：Microbiology and immunology

authors： Tsuji A,Kaneko Y,Takahashi K,Ogawa M,Goto S

更新日期：1982-01-01 00:00:00

abstract：:We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV-induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric as...

journal_title：Microbiology and immunology

authors： Kodama E,Igarashi A,Mori S,Hashimoto K,Suzuki T,De Clercq E,Shigeta S

更新日期：1996-01-01 00:00:00

abstract：:In order to count Clostridium tyrobutyricum spores in milk after membrane filtration, murine 21E7-B12 monoclonal antibody was produced. Elution of the monoclonal antibody from this antigen, the flagellar filament protein, by carbohydrate ligands was used to study the epitope structure. A competitive elution of an anti...

journal_title：Microbiology and immunology

authors： Bédouet L,Arnold F,Robreau G,Batina P,Talbot F,Malcoste R

更新日期：1998-01-01 00:00:00

abstract：:Capture enzyme-linked immunosorbent assays (ELISAs) were developed to detect immunoglobulin G and M antibodies to group A streptococcal (GAS) antigens, streptolysin O, streptokinase, and group A carbohydrate. The sensitivities and the specificities of the IgM capture ELISAs to each GAS antigen were high enough to dist...

journal_title：Microbiology and immunology

authors： Hayashi S,Yokota K,Takizawa Y,Tomizawa I,Nejime T,Oguma K

更新日期：1993-01-01 00:00:00

abstract：:A polymyxin B (PXB)-resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB-resistant isolate bound little PXB, although LPS from the parental strain did. The 31P nuclear magnetic resonance (NMR) spectrum of PXB-resistant type LPS showed that it contained much less of...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Jiang GZ,Kato Y,Sugiyama T,Kawai M,Fukada M,Takahashi K

更新日期：1994-01-01 00:00:00

abstract：:Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

journal_title：Microbiology and immunology

authors： Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

更新日期：1993-01-01 00:00:00

abstract：:The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

journal_title：Microbiology and immunology

authors： Singh JP,Verma R

更新日期：2008-08-01 00:00:00

abstract：:Amino acid requirements for the growth of Aedes albopictus, clone C6/36, cells and for the production of dengue (DEN) and Chikungunya (CHIK) viruses were examined by growing the cells or the viruses in media which were deprived of one of the 20 amino acids. Cell growth was markedly inhibited when cystine was omitted f...

journal_title：Microbiology and immunology

authors： Sasao F,Igarashi A,Fukai K

更新日期：1980-01-01 00:00:00

abstract：:Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

journal_title：Microbiology and immunology

authors： Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

更新日期：2008-12-01 00:00:00

abstract：:A monoclonal antibody (MoAb) to human neutrophils, designated 3H9, was established by screening for the inhibition of neutrophil adherence to plastic plates containing a medium supplemented with fetal calf serum (FCS medium). The antigen recognized by 3H9 was shown to be present on human leukocytes and found at the hi...

journal_title：Microbiology and immunology

authors： Ohtake K,Takei H,Watanabe T,Sato Y,Yamashita T,Sudo K,Kuroki M,Chihara J,Sendo F

更新日期：1997-01-01 00:00:00

abstract：:Our objective was to study the alterations of CD4(+) CD25(+) Foxp3(+) T(regs) in HIV-infected SPs and to examine the role of T(regs) in the disease progression of HIV. The proportion of CD4(+) CD25(+) Foxp3(+) T(regs) in peripheral blood of 24 SPs, 30 asymptomatic HIV-infected patients, 20 AIDS patients, and 16 non-in...

journal_title：Microbiology and immunology

authors： Zhang Z,Jiang Y,Zhang M,Liu J,Sun G,Shi W,Wang Y,Shang H

更新日期：2010-10-01 00:00:00

abstract：:We previously reported a helper T-cell (Th) epitope (peptide i) which corresponded to the sequence ranging from positions 462 to 479 from the N-terminus of the Friend-murine leukemia virus (F-MuLV) envelope protein (env462-479). Homologous sequences exist in both Moloney-murine leukemia (M-MuLV env452-469) and endogen...

journal_title：Microbiology and immunology

authors： Uenishi H,Iwanami N,Yamagishi H,Nakatani T,Kawasaki T,Tamamura H,Fujii N,Kuribayashi K

更新日期：1998-01-01 00:00:00

abstract：:A human T cell hybridoma clone, F5, producing high levels of macrophage migration inhibitory factor (MIF) was established by the emetine-actinomycin D selection method. This clone produced two species of MIF which were separated on a Phenyl Sepharose column. We purified MIF-2 (the more hydrophobic species of the two) ...

journal_title：Microbiology and immunology

authors： Hirose S,Ooki S,Higuchi M,Osawa T

更新日期：1991-01-01 00:00:00

abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:Zymographic analysis was performed to know the bacteriolytic enzyme profiles of 4% SDS extracts of oral streptococci, Streptococcus mutans, S. sobrinus, S. sanguis, S. mitis and S. salivarius. We investigated the five strains in each species and found that the profile was very similar among strains of the same species...

journal_title：Microbiology and immunology

authors： Yoshimura G,Komatsuzawa H,Kajimura J,Fujiwara T,Ohara M,Kozai K,Sugai M

更新日期：2004-01-01 00:00:00

abstract：:BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA spec...

journal_title：Microbiology and immunology

authors： He F,Morita H,Kubota A,Ouwehand AC,Hosoda M,Hiramatsu M,Kurisaki J

更新日期：2005-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

journal_title：Microbiology and immunology

authors： Kim EH,Aoki T

更新日期：1994-01-01 00:00:00

abstract：:Streptococcus anginosus appears to be able to adhere to cultured epithelial cells or fibronectin and this may be associated with bacterial pathogenicity. In the present study, the molecular characteristics and virulence of the fibronectin-binding protein (FBP), Fbp62, of S. anginosus were investigated in animal models...

journal_title：Microbiology and immunology

authors： Kodama Y,Ishikawa T,Shimoyama Y,Sasaki D,Kimura S,Sasaki M

更新日期：2018-10-01 00:00:00

abstract：:Frauch's micro-slide method was improved to facilitate the endpoint-determination of the Limulus test. Two precise observations, by inverted phase contrast microscopy and with a staining procedure, were newly performed as additions to the slide test. The staining procedure was proposed as an improved method for the Li...

journal_title：Microbiology and immunology

authors： Okuguchi S

更新日期：1978-01-01 00:00:00

abstract：:The IFN-induced double-stranded RNA (dsRNA)-activated protein kinase PKR is one of the key molecules in the antiviral effects of IFN. To clarify the effects of hepatitis C virus nonstructural protein 5A (NS5A) on antiviral activity of IFN, in particular on PKR kinase activity, in mammalian cells, we established induci...

journal_title：Microbiology and immunology

authors： Noguchi T,Satoh S,Noshi T,Hatada E,Fukuda R,Kawai A,Ikeda S,Hijikata M,Shimotohno K

更新日期：2001-01-01 00:00:00

abstract：:The cell wall of Clostridium difficile GAI 4131 was revealed by electron microscopy to have an outer layer composed of a nearly square array and contained the two major proteins with molecular weights of 38 kDa and 42 kDa. The properties and reassembly of the two major proteins into the regular array were investigated...

journal_title：Microbiology and immunology

authors： Masuda K,Itoh M,Kawata T

更新日期：1989-01-01 00:00:00

abstract：:A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. As in the antibiotic sensitivity test, filter paper disks containing defined amounts of bile salt were placed on a lawn of bacterial cells ...

journal_title：Microbiology and immunology

authors： Yamazaki E,Sugimoto K,Niwano K,Okada J

更新日期：1982-01-01 00:00:00

abstract：:Botulinum neurotoxin (BoNT) is the causative agent of botulism in humans and animals. Only BoNT serotype A subtype 1 (BoNT/A1) is used clinically because of its high potency and long duration of action. BoNT/A1 and BoNT/A subtype 2 (BoNT/A2) have a high degree of amino acid sequence similarity in the light chain (LC) ...

journal_title：Microbiology and immunology

authors： Kohda T,Tsukamoto K,Torii Y,Kozaki S,Mukamoto M

更新日期：2020-07-01 00:00:00

abstract：:Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Iwata H,Miyadai T,Kimura Y

更新日期：1988-01-01 00:00:00

abstract：:An emetic toxin cereulide, produced by Bacillus cereus, causes emetic food poisonings, but a method for quantitative measurement of cereulide has not been well established. A current detection method is a bioassay method using the HEp-2 cell vacuolation test, but it was unable to measure an accurate concentration. We ...

journal_title：Microbiology and immunology

authors： Kawamura-Sato K,Hirama Y,Agata N,Ito H,Torii K,Takeno A,Hasegawa T,Shimomura Y,Ohta M

更新日期：2005-01-01 00:00:00