Abstract:
:Amino acid requirements for the growth of Aedes albopictus, clone C6/36, cells and for the production of dengue (DEN) and Chikungunya (CHIK) viruses were examined by growing the cells or the viruses in media which were deprived of one of the 20 amino acids. Cell growth was markedly inhibited when cystine was omitted from the medium, and to a lesser extent by arginine deprivation. On the other hand, omission of alanine, asparagine, aspartic acid, and glutamic acid at the same time did not affect cell growth. Marked accumulation of alanine was observed in the medium when the cells were grown for 8 days in complete medium, with concomitant depletion of aspartic acid and glutamic acid. The production of CHIK virus was inhibited markedly by omission of cystine from the medium after virus infection, while the production of DEN viruses was more affected by glycine deprivation, although cystine deprivation also inhibited virus production to a lesser extent. On the other hand, production of CHIK and DEN viruses was not affected when alanine, asparagine, aspartic acid, and glutamic acid were omitted from the medium at the same time.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Sasao F,Igarashi A,Fukai Kdoi
10.1111/j.1348-0421.1980.tb02897.xsubject
Has Abstractpub_date
1980-01-01 00:00:00pages
915-24issue
10eissn
0385-5600issn
1348-0421journal_volume
24pub_type
杂志文章abstract::Irradiation treatment enhanced resistance of C57BL/6, but not BALB/c against Toxoplasma gondii infection. Six Gy-irradiated (IR) C57BL/6 recipients of B-2 cells from T. gondii-infected C57BL/6 died after infection. B-2 suppressor cells from infected C57BL/6 enhanced production of IL-4 and IL-10 in peritoneal exudate c...
journal_title:Microbiology and immunology
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abstract::In order to elucidate the molecular characteristics of Japanese encephalitis (JE) virus in Okinawa, 23 strains of JE virus isolated in a 25-year span were sequenced for the 240 nucleotides of the C-preM junction region and 111 nucleotides of the E gene region and compared with those of reference strains isolated in ma...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01150.x
更新日期:1996-01-01 00:00:00
abstract::Staphylococcus aureus Cowan I was incubated with monolayers of cells derived from several portions of mouse kidney, and found to be ingested by all types of the renal cells. Intracellular localization of S. aureus was determined by resistance of intracellular cocci against lysostaphin digestion and confirmed by electr...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1992.tb02042.x
更新日期:1992-01-01 00:00:00
abstract::Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In B. pseudomallei, RpoS has been reported to play ...
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02351.x
更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03144.x
更新日期:1987-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03277.x
更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01791.x
更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12367
更新日期:2016-04-01 00:00:00
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journal_title:Microbiology and immunology
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doi:10.1111/j.1348-0421.2004.tb03537.x
更新日期:2004-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2000.tb02513.x
更新日期:2000-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12249
更新日期:2015-05-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
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更新日期:2012-04-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2011.00353.x
更新日期:2011-08-01 00:00:00
abstract::We observed the effects of a chinese herb medicine Sho-saiko-to on the lethal and antitumor activities of recombinant human tumor necrosis factor (rhTNF) administered in mice. Sho-saiko-to was noted to protect the rhTNF-induced lethality in galactosamine-hypersensitized mice, and also Sho-saiko-to pretreated mice was ...
journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01091.x
更新日期:1996-01-01 00:00:00
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更新日期:2013-07-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00900.x
更新日期:1985-01-01 00:00:00
abstract::Four T antigen-positive phenotypic revertants were isolated by negative selection with BUdR from SV40-transformed rat and mouse cells which contain six and two viral genome equivalents per cell, respectively. Karyological analysis indicated that one rat and one mouse revertant had a hyperploid number of chromosomes, w...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1982.tb00151.x
更新日期:1982-01-01 00:00:00
abstract::We examined the role of B-1 cells in protection against Toxoplasma gondii infection using B cell-deficient mice (muMT mice). We found that primed but not naïve B-1 cells from wild-type C57BL/6 mice protected B cell-deficient recipients from challenge infection. All muMT mice transferred with primed B-1 cells survived ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
abstract::Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) an...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb03329.x
更新日期:1996-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02309.x
更新日期:1998-01-01 00:00:00
abstract::Trehalose 6,6'-dimycolate (TDM), or cord factor, is a crucial stimulus of immune responses during Mycobacterium tuberculosis infection. Although TDM has immuno-stimulatory properties, including adjuvant activity and the ability to induce granuloma formation, the mechanisms underlying these remain unknown. We hypothesi...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01186.x
更新日期:1997-01-01 00:00:00
abstract::The inflammasome is a multiprotein complex that mediates caspase-1 activation with subsequent maturation of the proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is known to be activated by Staphylococcus aureus, one of the leading causes of bacteremia worldwide. Inflammasome activation and regulation ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12738
更新日期:2019-12-01 00:00:00