Abstract：
:The cell wall of Clostridium difficile GAI 4131 was revealed by electron microscopy to have an outer layer composed of a nearly square array and contained the two major proteins with molecular weights of 38 kDa and 42 kDa. The properties and reassembly of the two major proteins into the regular array were investigated. When the isolated cell walls were treated with hydrophobic bond-disrupting agents or a chelating agent specific for Ca2+, the two major proteins were effectively removed and the regularly arranged outer layer disappeared. The amino acid composition of the two major proteins differed from each other. The two major proteins also gave different peptide maps from each other upon proteolysis with Staphylococcus aureus V8 protease. The major proteins solubilized from the isolated cell walls with 8 M urea or 4 M guanidine hydrochloride could be reassembled into open-ended cylinders possessing the native regular pattern by dialysis against neutral buffer containing 5 mM CaCl2. The reassembled cylinders purified by centrifugation on a Percoll density gradient were composed of almost equal amounts of the 38 kDa and 42 kDa proteins and freed from the other proteins. These results suggest that the regular array in the outer cell wall layer is constructed from the two major cell wall proteins and requires Ca2+ for its assembly.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Masuda K,Itoh M,Kawata Tdoi
10.1111/j.1348-0421.1989.tb01978.xsubject
Has Abstractpub_date
1989-01-01 00:00:00pages
287-98issue
4eissn
0385-5600issn
1348-0421journal_volume
33pub_type
杂志文章abstract：:Effects of each of three indigenous Lactobacillus groups on other bacterial populations were separately investigated in gnotobiotic rats. In the wall of the non-glandular part of the stomach, contents of the stomach and contents of the upper part of the small intestine, some pre-associated indigenous bacteria were red...
journal_title：Microbiology and immunology
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doi：10.1111/j.1348-0421.1977.tb00315.x
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journal_title：Microbiology and immunology
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doi：10.1111/j.1348-0421.1986.tb03014.x
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：1979-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1981.tb00099.x
更新日期：1981-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.2004.tb03489.x
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：1999-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：1980-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1996.tb01150.x
更新日期：1996-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：2001-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1988.tb01468.x
更新日期：1988-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：2005-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.2008.00004.x
更新日期：2008-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/1348-0421.12059
更新日期：2013-07-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.2000.tb02536.x
更新日期：2000-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1348-0421.2002.tb02734.x
更新日期：2002-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1984.tb00787.x
更新日期：1984-01-01 00:00:00
abstract：:Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In B. pseudomallei, RpoS has been reported to play ...
journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-05-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1348-0421.1984.tb00679.x
更新日期：1984-01-01 00:00:00
abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...
journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1993.tb03198.x
更新日期：1993-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/1348-0421.12476
更新日期：2017-04-01 00:00:00
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journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1993.tb01679.x
更新日期：1993-01-01 00:00:00
abstract：:M protein is an important virulence determinant in Streptococcus pyogenes, but the amounts of M protein in various strains of the species remain to be elucidated. To assess the amount of M protein in strains of each emm genotype, dot blot analysis was performed on 141 clinically isolated strains. Among the cell membra...
journal_title：Microbiology and immunology
pub_type： 杂志文章
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更新日期：2011-06-01 00:00:00
abstract：:A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin-poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18-48 hr after endotoxin injection than in the control mice. On the ot...
journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1984.tb00704.x
更新日期：1984-01-01 00:00:00
abstract：:There is a deficiency in the data concerning the role of hMPV in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hMPV in adults in our region. In the present study the association of hMPV with varieties of lower respiratory tract disorders in imm...
journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.2009.00162.x
更新日期：2009-11-01 00:00:00
abstract：:The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgG1 antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneou...
journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1983.tb00656.x
更新日期：1983-01-01 00:00:00
abstract：:A bacterial strain with affinity to Candida albicans was successfully obtained from a natural environment. An uncovered Petri dish containing a suspension of heat-killed C. albicans cells was allowed to stand in a laboratory for several days. Some bacteria which had adhered to the candidal cells were tested for their ...
journal_title：Microbiology and immunology
pub_type： 杂志文章
doi：10.1111/j.1348-0421.1998.tb02289.x
更新日期：1998-01-01 00:00:00
