Route-related variation in the immunogenicity of killed Salmonella enteritidis vaccine: role of antigen presenting cells.

abstract：:Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

journal_title：Microbiology and immunology

authors： Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

更新日期：2015-12-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5B (NS5B) is an RNA replicase. We expressed full-length NS5B (591 amino acid residues) in Escherichia coli as a fusion protein with maltose binding protein (MBP-NS5B). MBP-NS5B was recovered in the soluble fraction after centrifugation at 40,000 x g and affinity-purified ...

journal_title：Microbiology and immunology

authors： Tanaka T,Sugiyama K,Ikeda M,Naganuma A,Nozaki A,Saito M,Shimotohno K,Kato N

更新日期：2000-01-01 00:00:00

abstract：:Histopathological observations of changes at the injection site in mice were made for 20 weeks after a single intramuscular (im) injection of aluminum hydroxide-adsorbed tetanus toxoid (Alum-TT), plain tetanus toxoid (TT), or aluminum adjuvant (Alum alone). Marked injury to muscle fibers and infiltration of neutrophil...

journal_title：Microbiology and immunology

authors： Goto N,Akama K

更新日期：1982-01-01 00:00:00

abstract：:ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, ...

journal_title：Microbiology and immunology

authors： Imaizumi T,Mechti N,Matsumiya T,Sakaki H,Kubota K,Yoshida H,Kimura H,Satoh K

更新日期：2008-01-01 00:00:00

abstract：:We have previously shown that immunization of mice with plasmid pMEa synthesizing Japanese encephalitis virus (JEV) envelope protein induced anti-JEV humoral and cellular immune responses. We now show that intra-muscular co-administration of mice with pMEa and pGM-CSF, encoding murine granulocyte-macrophage colony-sti...

journal_title：Microbiology and immunology

authors： Bharati K,Appaiahgari MB,Vrati S

更新日期：2005-01-01 00:00:00

abstract：:A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin-poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18-48 hr after endotoxin injection than in the control mice. On the ot...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Abe H,Sakaguchi O

更新日期：1984-01-01 00:00:00

abstract：:Diarrheal diseases are major causes of morbidity and mortality among children in developing countries. We have analyzed the causative agents of diarrhea in children under five years of age who resided in rural environments but attended a hospital in Malindi, a coastal town in Kenya. Bacterial diarrhea was found in 239...

journal_title：Microbiology and immunology

authors： Saidi SM,Iijima Y,Sang WK,Mwangudza AK,Oundo JO,Taga K,Aihara M,Nagayama K,Yamamoto H,Waiyaki PG,Honda T

更新日期：1997-01-01 00:00:00

abstract：:A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody wa...

journal_title：Microbiology and immunology

authors： Shaku F,Matsuda G,Furuya R,Kamagata C,Igarashi M,Tanaka M,Kanamori M,Nishiyama Y,Yamamoto N,Kawaguchi Y

更新日期：2005-01-01 00:00:00

abstract：:Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expre...

journal_title：Microbiology and immunology

authors： Na HN,Kim KH,Song MK,Park HL,Lee EY,Shim SH,Park S,Nam JH

更新日期：2013-09-01 00:00:00

abstract：:The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...

journal_title：Microbiology and immunology

authors： Nishimura H,Sugawara K,Gao P,Muraki Y,Hongo S,Kitame F,Nakamura K

更新日期：1995-01-01 00:00:00

abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:Temperature dependence of transfer was examined with ten R plasmids originating from clinical isolates of Salmonella. Six of the plasmids were thermosensitive upon transfer, five of which were originally harbored in S. typhimurium and the remaining one in S. derby. One of these plasmids, pNR502, which conferred resist...

journal_title：Microbiology and immunology

authors： Yoshida Y,Terawaki Y,Nakaya R

更新日期：1978-01-01 00:00:00

abstract：:Water-insoluble glucan (WIG) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. Glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze WIG. The pu...

journal_title：Microbiology and immunology

authors： Otsuka R,Imai S,Murata T,Nomura Y,Okamoto M,Tsumori H,Kakuta E,Hanada N,Momoi Y

更新日期：2015-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...

journal_title：Microbiology and immunology

authors： Otsuka N,Yoshino S,Kawano K,Toyoizumi-Ajisaka H,Shibayama K,Kamachi K

更新日期：2012-07-01 00:00:00

abstract：:Many studies have proved the relevance of local immune responses, rather than systemic immunity, to the pathogenesis of allergic rhinitis. Indeed, allergen-specific B lymphocyte undergoes class switching to IgE in situ. However, the relative contribution of in situ production to the amount in serum is still ambiguous....

journal_title：Microbiology and immunology

authors： Yoshida T,Usui A,Kusumi T,Inafuku S,Sugiyama T,Koide N,Yokochi T

更新日期：2005-01-01 00:00:00

abstract：:A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...

journal_title：Microbiology and immunology

authors： Ohyama T,Watanabe T,Fujinaga Y,Inoue K,Sunagawa H,Fujii N,Inoue K,Oguma K

更新日期：1995-01-01 00:00:00

abstract：:The nucleic acid fraction from cells of 6 species of bacterium and 2 kinds of vertebrate, calf and salmon, was extracted and purified by the same procedures as described previously. When the spleen cells from BALB/c mice were incubated with the nucleic acid fraction from either of the bacteria, natural killer (NK) act...

journal_title：Microbiology and immunology

authors： Yamamoto S,Yamamoto T,Shimada S,Kuramoto E,Yano O,Kataoka T,Tokunaga T

更新日期：1992-01-01 00:00:00

abstract：:To establish an in vitro method of predicting in vivo efficacy of antifungal drugs against Candida albicans and Aspergillus fumigatus, the antifungal activities of fluconazole, itraconazole, and amphotericin B were determined in mouse serum. The minimum inhibitory concentration (MIC) of each drug was measured using mo...

journal_title：Microbiology and immunology

authors： Maki K,Watabe E,Iguchi Y,Nakamura H,Tomishima M,Ohki H,Yamada A,Matsumoto S,Ikeda F,Tawara S,Mutoh S

更新日期：2006-01-01 00:00:00

abstract：:Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

journal_title：Microbiology and immunology

authors： Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

更新日期：2008-12-01 00:00:00

abstract：:Recipient cells migrating into the transplantation site of an allograft recognize histocompatibility antigens on the grafts and are cytotoxic against the grafts. Although the alloreactive immune response is predominantly directed at the major histocompatibility complex (major histocompatibility complex [MHC]; H-2 in m...

journal_title：Microbiology and immunology

authors： Inoue Y,Tashiro-Yamaji J,Hayashi M,Kiyonari H,Shimizu T,Ibata M,Yamana H,Kubota T,Tanigawa N,Yoshida R

更新日期：2011-06-01 00:00:00

abstract：:The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...

journal_title：Microbiology and immunology

authors： Tsuboi A,Oka Y,Osaki T,Kumagai T,Tachibana I,Hayashi S,Murakami M,Nakajima H,Elisseeva OA,Fei W,Masuda T,Yasukawa M,Oji Y,Kawakami M,Hosen N,Ikegame K,Yoshihara S,Udaka K,Nakatsuka S,Aozasa K,Kawase I,Sugiyama H

更新日期：2004-01-01 00:00:00

abstract：:Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...

journal_title：Microbiology and immunology

authors： Chosa T,Yamamoto N,Koyanagi Y,Kohno M,Nagata K,Hinuma Y

更新日期：1984-01-01 00:00:00

abstract：:One- and two-dimensional polyacrylamide gel electrophoresis (PAGE) was performed on immunoprecipitates formed between anti-human T-cell xeno-antiserum (ATS) and cell-surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a Lentil lectin-coupled Sepharose 4B column. ...

journal_title：Microbiology and immunology

authors： Fujimoto J,Ishii Y,Kon S,Matsuura A,Kikuchi K

更新日期：1981-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00

abstract：:Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

journal_title：Microbiology and immunology

authors： Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

更新日期：1996-01-01 00:00:00

abstract：:The effect of the two-year milk-feeding on the gastrointestinal (GI) microflora of a cynomolgus monkey was determined. Bifidobacterium spp. in the animal fed with cow's milk alone were distributed in higher number in the stomach, duodenum, cecum, and rectum. The increasingly higher numbers of Lactobacillus spp. and Ca...

journal_title：Microbiology and immunology

authors： Benno Y,Honjo S,Mitsuoka T

更新日期：1987-01-01 00:00:00

abstract：:The plasma concentration of soluble P-selectin (GMP-140/CD62P/PADGEM), a selectin produced by activated platelets and endothelial cells, was quantitated in a group of adults and East African negro children presenting with either non-severe or severe (cerebral) malaria caused by Plasmodium falciparum. Sixty percent of ...

journal_title：Microbiology and immunology

authors： Facer CA,Theodoridou A

更新日期：1994-01-01 00:00:00

abstract：:The concept of superantigens is well-known and widely accepted. In this brief communication, we analyze the behaviour of antigen-presenting cells after T-cell activation by staphylococcal enterotoxin B, a representative superantigen. We tried to activate murine T cells by inflammatory mouse peritoneal macrophage in th...

journal_title：Microbiology and immunology

authors： Sakurada J,Tajima A,Shinji H,Seki K,Masuda S

更新日期：2000-01-01 00:00:00

abstract：:The cell wall of Clostridium difficile GAI 4131 was revealed by electron microscopy to have an outer layer composed of a nearly square array and contained the two major proteins with molecular weights of 38 kDa and 42 kDa. The properties and reassembly of the two major proteins into the regular array were investigated...

journal_title：Microbiology and immunology

authors： Masuda K,Itoh M,Kawata T

更新日期：1989-01-01 00:00:00

abstract：:A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination me...

journal_title：Microbiology and immunology

authors： Murakami T,Hiraoka K,Mikami T,Matsumoto T,Katagiri S,Suzuki M

更新日期：1991-01-01 00:00:00