Detection sensitivity of influenza rapid diagnostic tests.

abstract：:Healthy cattle and swine bred in a district of Japan were examined for the presence of Clostridium botulinum in their liver. Liver specimens were cultivated in chopped meat-glucose medium and the cultures were examined for botulinum toxin. In cattle, none of the cultures of 100 liver specimens yielded the toxin. In sw...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Yoshimura K,Nakamura S

更新日期：1992-01-01 00:00:00

abstract：:A human T cell hybridoma clone, F5, producing high levels of macrophage migration inhibitory factor (MIF) was established by the emetine-actinomycin D selection method. This clone produced two species of MIF which were separated on a Phenyl Sepharose column. We purified MIF-2 (the more hydrophobic species of the two) ...

journal_title：Microbiology and immunology

authors： Hirose S,Ooki S,Higuchi M,Osawa T

更新日期：1991-01-01 00:00:00

abstract：:Borrelia duttonii strain 406K, a causative agent of relapsing fever, could not be cultivated in vitro in currently available media for borreliae. We have developed an in vitro cultivation system by using SflEp cell cultures. The average increases of the number of borreliae, when inoculated with 1.0 x 10(5) organisms p...

journal_title：Microbiology and immunology

authors： Konishi H,Morshed MG,Akitomi H,Nakazawa T

更新日期：1993-01-01 00:00:00

abstract：:Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on sixteen human isolates of serotype 2 of rotavirus in Japan, China, and Pakistan and their genetic variations were examined. Comparative studies of their nucleotide and deduced amino acid sequences between the sixteen is...

journal_title：Microbiology and immunology

authors： Wen L,Ushijima H,Kakizawa J,Fang ZY,Nishio O,Morikawa S,Motohiro T

更新日期：1995-01-01 00:00:00

abstract：:Murine B cell mitogens such as bacterial lipopolysaccharide (LPS), butanol-extracted water soluble adjuvant (Bu-WSA), dextran sulfate (DS), synthetic muramyl dipeptide (MDP), and its analog MDP-Lys (L18) do not show any mitogenic ability in vitro on human peripheral blood lymphocytes or mixed cell populations of purif...

journal_title：Microbiology and immunology

authors： Nitta T,Konno-Ejiri H,Okumura S,Ozawa A,Nakano M

更新日期：1985-01-01 00:00:00

abstract：:We isolated a filamentous bacteriophage, VfO4K68, from the pandemic Vibrio parahaemolyticus strain belonging to 04:K68 serovar. The VfO4K68 DNA lacked a 1,893-bp fragment present in that of the distinctive region of f237, a filamentous phage isolated from a pandemic 03:K6 strain (Nasu, H. et al., J. Clin. Microbiol., ...

journal_title：Microbiology and immunology

authors： Chan B,Miyamoto H,Taniguchi H,Yoshida S

更新日期：2002-01-01 00:00:00

abstract：:Based on the results of a phylogenetic analysis of 16S rRNA and the presence of sphingoglycolipid in cellular lipids of the type strains, transfer of "Rhizomonas" suberifaciens, Blastomonas natatoria and Erythromonas ursincola to the genus Sphingomonas as Sphingomonas suberifaciens (van Bruggen et al 1990) comb. nov.,...

journal_title：Microbiology and immunology

authors： Yabuuchi E,Kosako Y,Naka T,Suzuki S,Yano I

更新日期：1999-01-01 00:00:00

abstract：:SCG is a major 6-branched 1,3-beta-D-glucan in Sparassis crispa Fr. SCG shows antitumor activity and also enhances the hematopoietic response in cyclophosphamide (CY)-treated mice. In the present study, the molecular mechanism of the enhancement of the hematopoietic response was investigated. The levels of interferon-...

journal_title：Microbiology and immunology

authors： Harada T,Kawaminami H,Miura NN,Adachi Y,Nakajima M,Yadomae T,Ohno N

更新日期：2006-01-01 00:00:00

abstract：:A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination me...

journal_title：Microbiology and immunology

authors： Murakami T,Hiraoka K,Mikami T,Matsumoto T,Katagiri S,Suzuki M

更新日期：1991-01-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin-poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18-48 hr after endotoxin injection than in the control mice. On the ot...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Abe H,Sakaguchi O

更新日期：1984-01-01 00:00:00

abstract：:Vibrio hollisae strains isolated recently from patients in various locations were examined for the presence of the thermostable direct hemolysin gene (tdh) using nucleic acid hybridization and polymerase chain reaction assays. The results were consistent with the previous finding that all strains of V. hollisae carry ...

journal_title：Microbiology and immunology

authors： Nishibuchi M,Janda JM,Ezaki T

更新日期：1996-01-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00

abstract：:A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

journal_title：Microbiology and immunology

authors： Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

更新日期：1999-01-01 00:00:00

abstract：:Cytochalasin B (CB) and cytochalasin D (CD), inhibitors of microfilament function of host cell, were examined for their effects on Staphylococcus aureus Cowan I adherence to and ingestion by several types of the hyperosmolarity-tolerant (HOT) cells obtained from primary culture of mouse kidney. Staphylococcal adherenc...

journal_title：Microbiology and immunology

authors： Murai M,Seki K,Sakurada J,Usui A,Masuda S

更新日期：1993-01-01 00:00:00

abstract：:Quantitative PCR is becoming widespread for diagnosing and monitoring post-transplantation diseases associated with EBV and CMV. These assays need to be standardized to manage patients in different facilities. Five independent laboratories in Japan compared home-brew assays and a prototype assay system to establish a ...

journal_title：Microbiology and immunology

authors： Ito Y,Takakura S,Ichiyama S,Ueda M,Ando Y,Matsuda K,Hidaka E,Nakatani K,Nishioka J,Nobori T,Kajiyama N,Kimura H

更新日期：2010-09-01 00:00:00

abstract：:The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

journal_title：Microbiology and immunology

authors： Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

更新日期：2012-01-01 00:00:00

abstract：:We have developed a convenient method for assaying the receptor-destroying enzyme (RDE) activity of influenza C virus. This method measures the ability of the RDE to destroy the hemagglutination-inhibition activity of a potent inhibitor present in rat serum. Some physico-chemical properties of the RDE of influenza C v...

journal_title：Microbiology and immunology

authors： Sugawara K,Kitame F,Homma M,Nakamura K

更新日期：1985-01-01 00:00:00

abstract：:A leukocidin was isolated and purified from autolysates of Pseudomonas aeruginosa strain 158 by a combination of procedures such as column chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-100, and zone electrophoresis on pevikon. The purified preparation showed a single band in each experiment using ...

journal_title：Microbiology and immunology

authors： Hirayama T,Kato I,Matsuda F,Noda M

更新日期：1983-01-01 00:00:00

abstract：:The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17-kDa antigen gene of Rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the Katayama, Abe, Misaka and Kojima strains,...

journal_title：Microbiology and immunology

authors： Tange Y,Matsumoto M,Okada T,Hasegawa H,Kobayashi Y

更新日期：1994-01-01 00:00:00

abstract：:This study investigated the mechanisms of resistance of 36 quinolone-resistant Salmonella Typhimurium strains isolated from outpatients with infectious diarrhea in Beijing Tian Tan Hospital between 2013 and 2015. The resistance spectrum of the 36 strains was measured using a broth dilution method. Class 1 integrons ha...

journal_title：Microbiology and immunology

authors： Yuan J,Guo W

更新日期：2017-04-01 00:00:00

abstract：:Nineteen strains of Newcastle disease virus (NDV) isolated from wild ducks in Japan were placed into 4 distinct antigenic groups on the basis of their reactivities to 8 monoclonal antibodies against the HN molecule of NDV in hemagglutination inhibition tests. The NDV strains of duck origin were antigenically distinct ...

journal_title：Microbiology and immunology

authors： Kawamura M,Nagata-Matsubara K,Nerome K,Yamane N,Kida H,Kodama H,Izawa H,Mikami T

更新日期：1987-01-01 00:00:00

abstract：:There are many examples of probiotic effects of various lactic bacteria on enteropathogens. In this study, Lactobacillus strains (L. rhamnosus, L. gasseri, L. casei and L. plantarum) were tested in an in vitro model of enterohemorrhagic Escherichia coli (EHEC) infection of a human colon epithelial cell line, C2BBe1. W...

journal_title：Microbiology and immunology

authors： Hirano J,Yoshida T,Sugiyama T,Koide N,Mori I,Yokochi T

更新日期：2003-01-01 00:00:00

abstract：:Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of ...

journal_title：Microbiology and immunology

authors： Wang R,Chen S,Jin J,Ren F,Li Y,Qiao Z,Wang Y,Zhao L

更新日期：2015-05-01 00:00:00

abstract：:One- and two-dimensional polyacrylamide gel electrophoresis (PAGE) was performed on immunoprecipitates formed between anti-human T-cell xeno-antiserum (ATS) and cell-surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a Lentil lectin-coupled Sepharose 4B column. ...

journal_title：Microbiology and immunology

authors： Fujimoto J,Ishii Y,Kon S,Matsuura A,Kikuchi K

更新日期：1981-01-01 00:00:00

abstract：:During 1985 to 1990, serum samples were obtained from 229 healthy dogs. The dogs lived in Hokkaido, known to be infested with ticks. An enzyme-linked immunosorbent assay (ELISA) was used to detect IgG and IgM antibodies against Borrelia burgdorferi HO14 and HP3, which were isolated from Ixodes ovatus and I. persulcatu...

journal_title：Microbiology and immunology

authors： Isogai E,Isogai H,Sato N,Yuzawa M,Kawakami M

更新日期：1990-01-01 00:00:00

abstract：:The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric ...

journal_title：Microbiology and immunology

authors： Damodharan S,Gujar R,Pattabiraman S,Nesakumar M,Hanna LE,Vadakkuppattu RD,Usha R

更新日期：2013-05-01 00:00:00

abstract：:Using a new plaque cloning technique, we obtained unique Chlamydia trachomatis strains which were confirmed to be free of the 7.5-kb common plasmid and glycogen in inclusions. The in vitro susceptibility of these strains to various chemotherapeutic agents was tested by comparison with their parent strains and clinical...

journal_title：Microbiology and immunology

authors： Miyashita N,Matsumoto A,Matsushima T

更新日期：2000-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and laste...

journal_title：Microbiology and immunology

authors： Kuramoto E,Toizumi S,Shimada S,Tokunaga T

更新日期：1989-01-01 00:00:00