Fate of Legionella pneumophila in macrophages of C57BL/6 chronic granulomatous disease mice.

Abstract:

:We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in vitro phagocytosis, but not in the CGD macrophages. Thereafter, there was a 10-fold increase of CFU in A/J macrophages. The bacteria, however, did not proliferate in C57BL/6 and CGD macrophages at 24 or 48 hr after in vitro phagocytosis. These results suggest that effector molecules for the initial killing are a superoxide anion and its metabolites, and Lgn1 gene product inhibits the intracellular growth of L. pneumophila independently of NADPH oxidase.

journal_name

Microbiol Immunol

authors

Saito M,Kajiwara H,Miyamoto H,Yoshida S

doi

10.1111/j.1348-0421.2001.tb02655.x

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

539-41

issue

7

eissn

0385-5600

issn

1348-0421

journal_volume

45

pub_type

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