Abstract:
:We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in vitro phagocytosis, but not in the CGD macrophages. Thereafter, there was a 10-fold increase of CFU in A/J macrophages. The bacteria, however, did not proliferate in C57BL/6 and CGD macrophages at 24 or 48 hr after in vitro phagocytosis. These results suggest that effector molecules for the initial killing are a superoxide anion and its metabolites, and Lgn1 gene product inhibits the intracellular growth of L. pneumophila independently of NADPH oxidase.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Saito M,Kajiwara H,Miyamoto H,Yoshida Sdoi
10.1111/j.1348-0421.2001.tb02655.xkeywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
539-41issue
7eissn
0385-5600issn
1348-0421journal_volume
45pub_type
杂志文章abstract::We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for Lyme disease was compared with that of a conventional indirect immunoperoxidase tec...
journal_title:Microbiology and immunology
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doi:10.1111/j.1348-0421.1991.tb01590.x
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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更新日期:1997-01-01 00:00:00
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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