Biological activities of lipopolysaccharide-like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton.

abstract：:Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...

journal_title：Microbiology and immunology

authors： Ohyama T,Yoshizumi S,Sawada H,Uchiyama Y,Katoh Y,Hamaoka N,Utagawa E

更新日期：1999-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) an arthropod-borne Alphavirus is responsible for chikungunya fever and joint pain etc. Arthralgia is a major symptom; some patients recover early while others suffer for a very long time. Epidemiology and molecular characterization of three whole-genome sequences of CHIKV have been carried ou...

journal_title：Microbiology and immunology

authors： Khan N,Bhat R,Jain V,Raghuvendara B S,Patel AK,Ray P

更新日期：2020-12-21 00:00:00

abstract：:The function of MHC class II HLA-DR molecules expressed on a human B lymphoid progenitor cell line FL8.2.4.4 (abbreviated as FL4.4) was examined. FL4.4 cells expressed HLA-DR molecules and stimulation of the DR molecules by anti-DR mAb or by superantigen TSST-1 induced strong augmentation of homocytic aggregation and ...

journal_title：Microbiology and immunology

authors： Naitoh K,Ichigi Y,Miyake K,Muraguchi A,Kimoto M

更新日期：1994-01-01 00:00:00

abstract：:We have previously reported a mouse model of severe group A streptococcal infection (Microbiol. Immunol. 45: 777-786, 2001). When we injected Streptococcus pyogenes strains intramuscularly, the mice suffered from acute phase of infection for a few days but recovered from the illness and gained body weight. These mice,...

journal_title：Microbiology and immunology

authors： Iida K,Seki M,Saito M,Kawamura Y,Kajiwara H,Yoshida S

更新日期：2006-01-01 00:00:00

abstract：:Virulence of Coxsackie virus B3 (CB3) was compared in mice between strain SK-74 isolated from a patient and strain T-70 isolated from a healthy child as well as between prototype strain Nancy and its mouse-passaged derivative strain PMH. Strain SK-74 showed a high mortality (40-80%), while strain T-70 did not induce d...

journal_title：Microbiology and immunology

authors： Komatsu T,Hashimoto I,Kohara T

更新日期：1983-01-01 00:00:00

abstract：:A chemotaxonomic study was carried out on 31 strains of non-O1 Vibrio cholerae bio-serogroup Hakata, isolated in Japan, which possesses the Inaba antigen C of O1 V. cholerae. On the basis of the compositional sugar pattern of the polysaccharide portion of their lipopolysaccharides, the 23 strains isolated from the env...

journal_title：Microbiology and immunology

authors： Kondo S,Iguchi T,Haishima Y,Isshiki Y,Hisatsune K

更新日期：1989-01-01 00:00:00

abstract：:Neonatal toxic shock syndrome-like exanthematous disease (NTED) is a new entity of methicillin-resistant Staphylococcus aureus (MRSA) infection. Most of NTED cases reported previously in the literature were sporadic ones. In the present report, we describe an outbreak of NTED that occurred in a neonatal intensive care...

journal_title：Microbiology and immunology

authors： Nakano M,Miyazawa H,Kawano Y,Kawagishi M,Torii K,Hasegawa T,Iinuma Y,Ohta M

更新日期：2002-01-01 00:00:00

abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:The protective role of neutrophils on intranasal infection of influenza virus was investigated in 3 strains of tumor-bearing mice with neutrophilic leukocytosis. In vitro multiplication of influenza virus was inhibited by neutrophils from both normal and tumor-bearing mice, and the inhibitory effect of neutrophils was...

journal_title：Microbiology and immunology

authors： Fujisawa H

更新日期：2001-01-01 00:00:00

abstract：:Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/I...

journal_title：Microbiology and immunology

authors： Guth BE,Aguiar EG,Griffin PM,Ramos SR,Gomes TA

更新日期：1994-01-01 00:00:00

abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

journal_title：Microbiology and immunology

authors： Nair GB,Bhattacharya SK,Takeda T

更新日期：1993-01-01 00:00:00

abstract：:We determined the effect of heat-stable enterotoxin produced by Yersinia enterocolitica (Y. enterocolitica ST) on cyclic nucleotide levels in the intestines of 6-day-old mice and in cultured cell line cells. The concentration of cyclic guanosine 3',5'-monophosphate (cyclic GMP) in homogenates of the intestines increas...

journal_title：Microbiology and immunology

authors： Inoue T,Okamoto K,Moriyama T,Takahashi T,Shimizu K,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA spec...

journal_title：Microbiology and immunology

authors： He F,Morita H,Kubota A,Ouwehand AC,Hosoda M,Hiramatsu M,Kurisaki J

更新日期：2005-01-01 00:00:00

abstract：:The concept of superantigens is well-known and widely accepted. In this brief communication, we analyze the behaviour of antigen-presenting cells after T-cell activation by staphylococcal enterotoxin B, a representative superantigen. We tried to activate murine T cells by inflammatory mouse peritoneal macrophage in th...

journal_title：Microbiology and immunology

authors： Sakurada J,Tajima A,Shinji H,Seki K,Masuda S

更新日期：2000-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...

journal_title：Microbiology and immunology

authors： Otsuka N,Yoshino S,Kawano K,Toyoizumi-Ajisaka H,Shibayama K,Kamachi K

更新日期：2012-07-01 00:00:00

abstract：:Cryptosporidium spp. is a major cause of diarrhea in developing countries, mainly affecting people with compromised immune systems in general and HIV-infected individuals with low CD4 + T-cell counts in particular. This infection is self-limiting in healthy persons; however, it can be severe, progressive and persisten...

journal_title：Microbiology and immunology

authors： Izadi M,Jonaidi-Jafari N,Saburi A,Eyni H,Rezaiemanesh MR,Ranjbar R

更新日期：2012-12-01 00:00:00

abstract：:Temperature dependence of transfer was examined with ten R plasmids originating from clinical isolates of Salmonella. Six of the plasmids were thermosensitive upon transfer, five of which were originally harbored in S. typhimurium and the remaining one in S. derby. One of these plasmids, pNR502, which conferred resist...

journal_title：Microbiology and immunology

authors： Yoshida Y,Terawaki Y,Nakaya R

更新日期：1978-01-01 00:00:00

abstract：:RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susce...

journal_title：Microbiology and immunology

authors： Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

更新日期：1997-01-01 00:00:00

abstract：:Vibrio vulnificus infection has attracted special interest because of its high mortality. A strong clinical association exists between hepatic dysfunction and increased morbidity and mortality from V. vulnificus infection. In this study, the effect of C-reactive protein (CRP), a typical hepatogenic acute phase protein...

journal_title：Microbiology and immunology

authors： Chae MR,Park BH,Kim JS,Rho HW,Park JW,Kim HR

更新日期：2000-01-01 00:00:00

abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI) is an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). TAFI in plasma is presumed to influence vascular disease in its role as a fibrinol...

journal_title：Microbiology and immunology

authors： Akatsu H,Ishiguro M,Ogawa N,Kanesaka T,Okada N,Yamamoto T,Campbell W,Okada H

更新日期：2007-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights wer...

journal_title：Microbiology and immunology

authors： Konishi E,Hotta S

更新日期：1980-01-01 00:00:00

abstract：:We attempted to detect Coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (PCR) method. Ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near Sapporo, Hokkaido. C. burnetii was detected in...

journal_title：Microbiology and immunology

authors： Yanase T,Muramatsu Y,Inouye I,Okabayashi T,Ueno H,Morita C

更新日期：1998-01-01 00:00:00

abstract：:A chimeric PCV1-2 clone containing the PCV2 capsid gene cloned into the backbone of the nonpathogenic PCV1 genome was recently generated based on PCV2 and PCV1 strains isolated in China. The efficacy of this available candidate inactivated vaccine was evaluated by subjecting conventional pigs to intramuscular immuniza...

journal_title：Microbiology and immunology

authors： Xujie L,Xiaobo W,Yi S,Jing F,Song G,Xiufan L

更新日期：2011-04-01 00:00:00

abstract：:Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Lamin...

journal_title：Microbiology and immunology

authors： Li Z,Cui B,Liu X,Wang L,Xian Q,Lu Z,Liu S,Cao Y,Zhao Y

更新日期：2020-03-01 00:00:00

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:The combination of a Legionella pneumophila culture isolation technique and macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction (PCR) is pivotal for effective routine use in an environmental water system laboratory. Detection of Legionella organisms in 169 environmental samples was ...

journal_title：Microbiology and immunology

authors： Nintasen R,Utrarachkij F,Siripanichgon K,Bhumiratana A,Suzuki Y,Suthienkul O

更新日期：2007-01-01 00:00:00

abstract：:TtT/M-87 cell is a macrophage cell line established from thyrotropic pituitary tumor tissues in mouse. In this paper, we report the immunological properties of M-87 cells as a model of tumor-associated macrophage. Contrasting with resident peritoneal macrophages, M-87 cells constitutively secreted small but significan...

journal_title：Microbiology and immunology

authors： Mitsuishi T,Akuzawa Y,Sato S,Rui J,Kodama K,Inoue K,Kurashige S

更新日期：1993-01-01 00:00:00

abstract：:An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

更新日期：1985-01-01 00:00:00

abstract：:In the present study, 12 patients with fever of undetermined origin, anemia and icterus were diagnosed with hemoplasma infection by light microscopy, transmission electron microscopy examination and PCR assay after being excluded from other usual febrile diseases. Complement receptor type I (CR1, CD35) expression on t...

journal_title：Microbiology and immunology

authors： Congbin Y,Aibin L,Congli Y,Juan X,Lanjun B,Weiping Z,Zhibiao Y,Xiuguo H

更新日期：2010-08-01 00:00:00