Rapid and sensitive PCR detection of Vibrio trachuri pathogenic to Japanese horse mackerel (Trachurus japonicus).

Abstract:

:A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specifically amplified the DNAs from V. trachuri T9210, T9213, and T9216 but not of those other bacterial strains. PCR using a Pst I-1 primer set made it possible to detect 100 fg of T9210 DNA. The PCR method reported here may be useful for detection and identification of V. trachuri pathogenic to Japanese horse mackerel.

journal_name

Microbiol Immunol

authors

Iwamoto Y,Suzuki Y,Kurita A,Watanabe Y,Shimizu T,Ohgami H,Yanagihara Y

doi

10.1111/j.1348-0421.1995.tb03290.x

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

1003-6

issue

12

eissn

0385-5600

issn

1348-0421

journal_volume

39

pub_type

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