Involvement of sialic acid in high-affinity uptake of dopamine by synaptosomes from rat brain.

Abstract:

:The high-affinity, sodium-dependent uptake of dopamine (DA) was inhibited by the pretreatment of synaptosomes with neuraminidase from Vibrio cholerae. The inhibition was of a non-competitive type, resulting in a 40% decrease of Vmax. Neither basal nor depolarization-stimulated release of DA was affected. Treatment of synaptosomes with neuraminidase caused a 48% loss of sialic acid from the lipid-bound pool and a 80% decrease in the protein-bound fraction. The inhibition of DA uptake was found to be related linearly to the loss of sialic acid from the protein pool. It is postulated that a sialic acid moiety is involved in DA transport across the synaptosomal membrane.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Zaleska MM,Erecińska M

doi

10.1016/0304-3940(87)90179-0

subject

Has Abstract

pub_date

1987-11-10 00:00:00

pages

107-12

issue

1

eissn

0304-3940

issn

1872-7972

pii

0304-3940(87)90179-0

journal_volume

82

pub_type

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