Abstract:
:Aim: The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical Candida spp. in planktonic and biofilm form. Materials & methods: The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests were performed to investigate the probable mechanism of action and the comet test and cytotoxicity test were applied to evaluate DNA damage. Results: Midazolam (MIDAZ) showed antifungal activity against planktonic cells (125-250 μg/ml) and reduced the viability of Candida spp. biofilms (125 a 2500 μg/ml). The interaction of MIDAZ against Candida spp. biofilms was observed through scanning electron microscopy, causing alteration of their appearance. Therefore, MIDAZ has antifungal potential against Candida spp.
journal_name
Future Microbioljournal_title
Future microbiologyauthors
Holanda MA,da Silva CR,de A Neto JB,do Av Sá LG,do Nascimento FB,Barroso DD,da Silva LJ,Cândido TM,Leitão AC,Barbosa AD,de Moraes MO,Cc B,Júnior HVNdoi
10.2217/fmb-2020-0080subject
Has Abstractpub_date
2021-01-01 00:00:00pages
71-81eissn
1746-0913issn
1746-0921journal_volume
16pub_type
杂志文章abstract::Carbapenem-resistant Acinetobacter baumannii (CRAB) is endemic in Greece. CRAB initially emerged in 2000 and since then, carbapenemases still have a crucial role in CRAB appearance, except for a few cases resulting from efflux pump or outer-membrane protein mechanisms. OXA-type carbapenemases present the highest preva...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb-2016-0200
更新日期:2017-07-01 00:00:00
abstract:AIM:To investigate the role of Candida albicans TUP1-mediated filamentation in the colonization of the mice gut. MATERIALS & METHODS:We used molecular genetics to generate a strain where filamentation is regulated by altering the expression of the TUP1 gene with tetracyclines. RESULTS:The colonization rates reached w...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0012
更新日期:2018-06-01 00:00:00
abstract:AIM:Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid nonin...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2016-0224
更新日期:2017-08-01 00:00:00
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journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.15.78
更新日期:2015-01-01 00:00:00
abstract::Traditionally, glycoproteins have been considered the exclusive property of eukaryotes and archaea, but it is now evident that glycoproteins are found in all domains of life. In recent years N-linked glycosylation among some epsilon-proteobacteria has emerged as a new and exciting research area and represents a useful...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.09.10
更新日期:2009-05-01 00:00:00
abstract:AIM:To compare two identification methods for coagulase-negative staphylococci (CoNS) isolated from patients with urinary tract infections, VITEK® 2 and MALDI-TOF VITEK®MS, with genotypic identification by internal transcribed spacer PCR (ITS-PCR). RESULTS:A total of 217 CoNS isolates were studied. Agreement of the VI...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0195
更新日期:2018-11-01 00:00:00
abstract:AIMS:This study aimed to evaluate the antimicrobial activity of two different formulations of bioglass BAG-S53P4 against multiresistant microorganisms involved in bone infections, and the capability of bioglass to select for resistance. METHODS:Antibacterial activity was evaluated by means of killing curves. The abili...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/FMB.15.57
更新日期:2015-01-01 00:00:00
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journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0070
更新日期:2018-02-01 00:00:00
abstract::Mycobacterium tuberculosis, the agent of TB, is a facultative intracellular bacterial pathogen that replicates inside host macrophages and other phagocytes within a membrane-bound vacuole or phagosome. How M. tuberculosis captures and exploits vital nutrients inside host cells is an intensive research area that might ...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.14.28
更新日期:2014-01-01 00:00:00
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journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.13.31
更新日期:2013-05-01 00:00:00
abstract::Aim: We sought to investigate the genetic epidemiological relatedness of carbapenem-resistant Acinetobacter baumannii (CRAB) strains of a suspected outbreak in a Lebanese tertiary care hospital to implement necessary infection prevention and control measures. Methods: Twenty-eight nonduplicate CRAB isolates detected a...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2020-0079
更新日期:2020-10-01 00:00:00
abstract::The term 'neglected tropical diseases' predominantly refers to single-entity, mostly parasitic diseases. However, a considerable morbidity and mortality burden is carried by patients infected with Gram-positive cocci and Gram-negative bacilli that are prevalent all over the world, yet have impact in tropical and devel...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.12.126
更新日期:2013-01-01 00:00:00
abstract::The past decade has seen a significant increase in the incidence of invasive fungal infections. The antifungal armamentarium for the treatment of serious fungal infections remains limited. A possible approach to overcoming antifungal drug resistance and high mortality rates seen in severe fungal infections is to combi...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.2.2.115
更新日期:2007-04-01 00:00:00
abstract::Cytomegalovirus (CMV) manifestations remain important complications after allogeneic hematopoietic stem cell transplantation (HSCT), even in the current era. Unfortunately, available anti-CMV agents, mainly viral polymerase inhibitors, have a substantial risk of myelosuppression and nephrotoxicity. Letermovir, a novel...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0250
更新日期:2019-02-01 00:00:00
abstract::Antibiotics are often used in neonates despite the absence of relevant dosing information in drug labels. For neonatal dosing, clinicians must extrapolate data from studies for adults and older children, who have strikingly different physiologies. As a result, dosing extrapolation can lead to increased toxicity or eff...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0058
更新日期:2017-09-01 00:00:00
abstract::Capabilities in mass spectrometry are evolving rapidly, with recent improvements in sensitivity, data analysis and, most important from the standpoint of this review, much higher throughput, allowing analysis of many samples in a single day. This short review describes how these improvements in mass spectrometry can b...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.3.6.625
更新日期:2008-12-01 00:00:00
abstract:AIM:To evaluate if radiation used in radiotherapy can cause changes in the virulence potential of Candida tropicalis ATCC 750. MATERIALS & METHODS:C. tropicalis was exposed in vitro to identical dose and scheme of irradiation would be used in patients with head and neck cancer. Some virulence parameters were analyzed ...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0137
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The seriousness to treat burn wounds infected with Pseudomonas aeruginosa led us to examine whether the effect of the carbapenem antibiotic imipenem is enhanced by hyperbaric oxygen (HBO). MATERIALS & METHODS:The effects of HBO (100% O2, 3 ATA, 5 h) in combination with imipenen on bacterial counts of six is...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.14.111
更新日期:2015-01-01 00:00:00
abstract::Breastfeeding is a major determinant of human health. Breast milk is not sterile and ecological large-scale sequencing methods have revealed an unsuspected microbial diversity that plays an important role. However, microbiological analysis at the species level has been neglected while it is a prerequisite before under...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0317
更新日期:2019-05-01 00:00:00
abstract::Aim:Cryptococcus neoformans is the major agent of cryptococcosis. The main virulence factor is the polysaccharide (PS) capsule. Changes in cryptococcal PS properties have been poorly elucidated. Materials & methods: We analyzed the mechanical properties of secreted PS and intact capsules, using dynamic light scatterin...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0320
更新日期:2019-07-01 00:00:00
abstract::Traditionally, the patients believed to be at highest risk of invasive aspergillosis (IA) are those who are neutropenic due to chemotherapy for hematological malignancy or those undergoing allogeneic hematopoietic stem cell transplantation. However, emerging data show that other patients are vulnerable to IA, even tho...
journal_title:Future microbiology
pub_type:
doi:10.2217/fmb.10.73
更新日期:2010-07-01 00:00:00
abstract::Aspergillus species are the most common causes of invasive mold infections in immunocompromised patients. The introduction of new antifungal agents and recent reports of resistance emerging during treatment of Aspergillus infections have highlighted the need for in vitro susceptibility testing. Various testing procedu...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.10.34
更新日期:2010-05-01 00:00:00
abstract::Coxiella burnetii is the etiological agent of Q fever, a worldwide zoonosis that can result in large outbreaks. The birth of genomics and sequencing of C. burnetii strains has revolutionized many fields of study of this infection. Accurate genotyping methods and comparative genomic analysis have enabled description of...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.15.137
更新日期:2016-01-01 00:00:00
abstract::Background: A major focus of tuberculosis drug discovery is aimed at the development of novel antibiotics with activity against drug-resistant strains of Mycobacterium tuberculosis. Results: We have synthesized ten isoniazid derivatives and investigated for antibacterial activity toward M. tuberculosis H37Rv and isoni...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0085
更新日期:2020-07-01 00:00:00
abstract:AIM:Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test. METHODS:We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0147
更新日期:2018-03-01 00:00:00
abstract:AIM:We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS:Staphylococcus argenteus was identified based on negative crtM gene and presence of specific s...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0015
更新日期:2018-07-01 00:00:00
abstract::Recognition of bacterial infection is the first key step to the initiation of an inflammatory response and host defense. Transmembrane proteins of the Toll-like receptor family have long been recognized as key detectors of the extracellular presence of pathogens. Recently, much research has identified a variety of int...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.4.1.65
更新日期:2009-02-01 00:00:00
abstract::Chronic hepatitis B virus infection is a global health concern as it affects over 240 million people worldwide and an estimated 686,000 people die annually as a result of complications of the disease. With the development of newer antiviral drugs, viral suppression of HBV is achievable, however elimination of HBV from...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb-2016-0128
更新日期:2016-12-01 00:00:00
abstract::Over the last decade Raman spectroscopy has become established as a physicochemical technique for the rapid identification of microbes. This powerful analytical method generates a spectroscopic fingerprint from the microbial sample, which provides quantitative and qualitative information that can be used to characteri...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.11.89
更新日期:2011-09-01 00:00:00
abstract::The Enterococcus species is the third main cause of infective endocarditis (IE) worldwide, and it is gaining relevance, especially among healthcare-associated cases. Patients with enterococcal IE are older and have more comorbidities than other types of IE. Classical treatment options are limited due to the emergence ...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.15.46
更新日期:2015-01-01 00:00:00