Understanding the structural mechanisms of antibiotic resistance sets the platform for new discovery.


:Understanding the structural basis of antibacterial resistance may enable rational design principles that avoid and subvert that resistance, thus leading to the discovery of more effective antibiotics. In this review, we explore the use of crystal structures to guide new discovery of antibiotics that are effective against resistant organisms. Structures of efflux pumps bound to substrates and inhibitors have aided the design of compounds with lower affinity for the pump or inhibitors that more effectively block the pump. Structures of β-lactamase enzymes have revealed the mechanisms of action toward key carbapenems and structures of gyrase have aided the design of compounds that are less susceptible to point mutations.


Future Microbiol


Future microbiology


Reeve SM,Lombardo MN,Anderson AC




Has Abstract


2015-01-01 00:00:00












  • Protective host immune responses to Salmonella infection.

    abstract::Salmonella enterica serovars Typhi and Paratyphi are the causative agents of human typhoid fever. Current typhoid vaccines are ineffective and are not widely used in endemic areas. Greater understanding of host-pathogen interactions during Salmonella infection should facilitate the development of improved vaccines to ...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Pham OH,McSorley SJ

    更新日期:2015-01-01 00:00:00

  • Pheromonicins: an ecologically sound family of bacteriocin-based antibiotics for use in the age of the microbiome.

    abstract::The time is ripe to usher in a new paradigm in infection control and to move beyond our sole reliance on broad-spectrum antibiotics whose use results in extensive collateral damage to our microbiome and, in so doing, exerts significant selective pressures for resistance to emerge. We propose to supplement the existing...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Qiu XQ,Roy SM,Riley MA

    更新日期:2015-01-01 00:00:00

  • Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in Greece: an extended review (2000-2015).

    abstract::Carbapenem-resistant Acinetobacter baumannii (CRAB) is endemic in Greece. CRAB initially emerged in 2000 and since then, carbapenemases still have a crucial role in CRAB appearance, except for a few cases resulting from efflux pump or outer-membrane protein mechanisms. OXA-type carbapenemases present the highest preva...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Karampatakis T,Antachopoulos C,Tsakris A,Roilides E

    更新日期:2017-07-01 00:00:00

  • Current status and future perspectives on molecular and serological methods in diagnostic mycology.

    abstract::Invasive fungal infections are an important cause of infectious morbidity. Nonculture-based methods are increasingly used for rapid, accurate diagnosis to improve patient outcomes. New and existing DNA amplification platforms have high sensitivity and specificity for direct detection and identification of fungi in cli...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Lau A,Chen S,Sleiman S,Sorrell T

    更新日期:2009-11-01 00:00:00

  • Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct.

    abstract:AIMS:Glucuronoxylomannan (GXM) is the major polysaccharide component of Cryptococcus neoformans. We evaluated in this study whether GXM fractions of different molecular masses were functionally distinct. MATERIALS & METHODS:GXM samples isolated from C. neoformans cultures were fractionated to generate polysaccharide p...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Albuquerque PC,Fonseca FL,Dutra FF,Bozza MT,Frases S,Casadevall A,Rodrigues ML

    更新日期:2014-01-01 00:00:00

  • Genotyping of Mycobacterium tuberculosis: application in epidemiologic studies.

    abstract::Genotyping is used to track specific isolates of Mycobacterium tuberculosis in a community. It has been successfully used in epidemiologic research (termed 'molecular epidemiology') to study the transmission dynamics of TB. In this article, we review the genetic markers used in molecular epidemiologic studies includin...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Kato-Maeda M,Metcalfe JZ,Flores L

    更新日期:2011-02-01 00:00:00

  • Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR.

    abstract::Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: A Castaneda C,Castillo M,Sanchez J,Casavilca S,Sanchez J,A Bernabe L,Suarez N,Chavez I,Ruiz E,Tello K,R Villa M,Zevallos R,Montenegro P,Dias-Neto E,Landa-Baella M,Taxa L

    更新日期:2020-08-01 00:00:00

  • Complementary roles of the Cek1 and Cek2 MAP kinases in Candida albicans cell-wall biogenesis.

    abstract:AIMS:To investigate whether Cek2 (as Cek1) is involved in the biogenesis of the fungal cell wall and to uncover differences and similitudes between both proteins. MATERIALS & METHODS:We used molecular genetics to characterize the role of Cek2 in MTL-heterozygous cells. RESULTS:Deletion of CEK2 (similar to CEK1) rende...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Correia I,Román E,Prieto D,Eisman B,Pla J

    更新日期:2016-01-01 00:00:00

  • Potential impact of removing metronidazole from treatment armamentarium of mild acute Clostridioides difficile infection.

    abstract::Aim: Recent guidelines recommended removing metronidazole as a therapeutic option for Clostridioides difficile infections (CDI). However, superiority of vancomycin over metronidazole in mild CDI is not established and use of vancomycin might lead to emergence of vancomycin-resistant enterococci (VRE). Patients & metho...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Zilberman-Itskovich S,Youngster I,Lazarovitch T,Bondarenco M,Toledano L,Kachlon Y,Mengesha B,Strul N,Zaidenstein R,Marchaim D

    更新日期:2019-11-01 00:00:00

  • The gut microbiome and the potentials of probiotics: an interview with Simon Gaisford.

    abstract::In this exclusive interview, Simon Gaisford discusses his current research into gut dysbiosis and its possible relevance to disease states, as well as the efficacy of probiotic supplementation and its possible use as treatment. This interview was conducted by Ellen Colvin, Commissioning Editor of Future Microbiology. ...

    journal_title:Future microbiology

    pub_type: 面试


    authors: Gaisford S

    更新日期:2019-03-01 00:00:00

  • Comparative analysis between biofilm formation and gene expression in Staphylococcus epidermidis isolates.

    abstract:AIM:To understand the relationship between ica, aap and bhp gene expression and the implications in biofilm formation in selected clinical and commensal Staphylococcus epidermidis isolates. MATERIAL & METHODS:Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, biofilm spati...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Freitas AI,Lopes N,Oliveira F,Brás S,França Â,Vasconcelos C,Vilanova M,Cerca N

    更新日期:2018-03-01 00:00:00

  • Virulence factors in pneumococcal respiratory pathogenesis.

    abstract::Streptococcus pneumoniae (the pneumococcus) is a major global cause of human disease. Since the publication of the entire sequence of TIGR4 in 2001, our understanding of this human pathogen has increased significantly. Genetic studies, and the use of mutant strains have refined our understanding of the pathogenic mech...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Preston JA,Dockrell DH

    更新日期:2008-04-01 00:00:00

  • Evaluation of different microtiter plate-based methods for the quantitative assessment of Staphylococcus aureus biofilms.

    abstract:AIM:To quantitatively assess Staphylococcus aureus biofilms. MATERIALS & METHODS:In addition to the qualitative Congo red agar (CRA) method, we used the bioluminescence (BLM), safranine (SAF), crystal violet (CRV) and resazurin (RES) high-throughput microtiter plate-based quantitative assays. RESULTS:60.47% (26/43) o...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Elkhatib WF,Khairalla AS,Ashour HM

    更新日期:2014-01-01 00:00:00

  • Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay.

    abstract:AIM:A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan. METHODS:The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical s...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Braun SD,Jamil B,Syed MA,Abbasi SA,Weiß D,Slickers P,Monecke S,Engelmann I,Ehricht R

    更新日期:2018-09-01 00:00:00

  • Endothelial cells and CMV dissemination.

    abstract::Using the murine CMV animal model and the well-established model of Cre-lox-P-mediated green-fluorescence tagging of endothelial cell (EC)-derived mouse CMV to quantify the role of infected ECs in transplantation-associated CMV dissemination (in mice expressing Cre recombinase under the control of either the Tie2 or t...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章


    authors: Gerna G

    更新日期:2012-04-01 00:00:00

  • Chronic fatigue syndrome, XMRV and blood safety.

    abstract::In the past few months, there has been public discussion relating to a new perspective on blood safety and specifically upon measures to prevent or discourage donation by individuals with a diagnosis of myalgic encephalopathy-chronic fatigue syndrome. This reflects an intriguing interplay between science, public healt...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Dodd RY

    更新日期:2011-04-01 00:00:00

  • Septic patients in the intensive care unit present different nasal microbiotas.

    abstract:AIM:The primary objective of this study was to evaluate correlations among mortality, intensive care unit (ICU) length of stay and airway microbiotas in septic patients. MATERIALS & METHODS:A deep-sequencing analysis of the 16S rRNA gene V4 region was performed. RESULTS:The nasal microbiota in septic patients was dom...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Tan XL,Liu HY,Long J,Jiang Z,Luo Y,Zhao X,Cai S,Zhong X,Cen Z,Su J,Zhou H

    更新日期:2019-03-01 00:00:00

  • Direct and indirect effects of the 13-valent pneumococcal conjugate vaccine administered to infants and young children.

    abstract::This paper analyzes the most recent reports regarding the direct and indirect effects of the 13-valent pneumococcal conjugate vaccine (PCV13) when administered to infants and young children. PCV13 extends the protective effects reported for the 7-valent pneumococcal conjugate vaccine (PCV7). A further reduction in the...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Esposito S,Principi N,ESCMID Vaccine Study Group.

    更新日期:2015-01-01 00:00:00

  • Know your enemy: managing resistant Gram-negative infections.

    abstract::Report from the 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2018), Madrid, Spain, 21-24 April 2018 Gram-negative bacteria such as Klebsiella, Acinetobacter and Pseudomonas cause some of the most serious infections and are increasingly resistant to multiple drugs and in some cases, t...

    journal_title:Future microbiology



    authors: Eckmann C,Rojas LJ,Lyon S

    更新日期:2018-10-01 00:00:00

  • Progress in the experimental therapy of severe arenaviral infections.

    abstract::A number of viruses in the family Arenaviridae cause severe illness in humans. Lassa virus in West Africa and a number of agents in South America produce hemorrhagic fever in persons exposed to aerosolized excretions of the pathogens' rodent hosts. Because arenaviruses are not transmitted by arthropods, and person-to-...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Gowen BB,Bray M

    更新日期:2011-12-01 00:00:00

  • Aspects of eukaryotic-like signaling in Gram-positive cocci: a focus on virulence.

    abstract::Living organisms adapt to the dynamic external environment for their survival. Environmental adaptation in prokaryotes is thought to be primarily accomplished by signaling events mediated by two-component systems, consisting of histidine kinases and response regulators. However, eukaryotic-like serine/threonine kinase...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Burnside K,Rajagopal L

    更新日期:2011-07-01 00:00:00

  • Human papillomavirus: gene expression, regulation and prospects for novel diagnostic methods and antiviral therapies.

    abstract::Human papillomaviruses (HPVs) cause diseases ranging from benign warts to invasive tumors. A subset of these viruses termed 'high risk' infect the cervix where persistent infection can lead to cervical cancer. Although many HPV genomes have been sequenced, knowledge of virus gene expression and its regulation is still...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Graham SV

    更新日期:2010-10-01 00:00:00

  • Retapamulin: a semisynthetic pleuromutilin compound for topical treatment of skin infections in adults and children.

    abstract::Retapamulin is a semisynthetic pleuromutilin compound with in vitroactivity against Gram-positive bacteria, no cross-resistance to other classes of antimicrobial agents in current use and a low potential for development of resistance. A 1% ointment formulation has been developed for clinical use, and a placebo-control...

    journal_title:Future microbiology

    pub_type: 临床试验,杂志文章


    authors: Jacobs MR

    更新日期:2007-12-01 00:00:00

  • Candida albicans vaginitis in a murine model is reduced by polypeptide-enriched Gastrodia elata extracts.

    abstract::Aim: The primary objective of this study was to evaluate the effects of polypeptide-enriched Gastrodia elata extracts (GE) on vulvovaginal candidiasis (VVC). Materials & methods: A VVC model induced by Candida albicans (C. albicans) infection was successfully developed in BALB/c mice. After treatment, the colony-formi...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Cai X,Kong F,Wang R,Zhai S,Guan X,Zhang G,Wang D

    更新日期:2019-07-01 00:00:00

  • Prediction of micafungin area under the curve data by using peak concentration: applicability and utility in antifungal therapy.

    abstract:AIM:To describe a predictive model to obtain the area under the plasma concentration versus time curve (AUC) for micafungin to aid in dosing strategies in pediatric patients. METHODS:Using published pharmacokinetic data a linear regression model to describe the Cmax versus AUCtau was developed. The mean absolute error...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Srinivas NR

    更新日期:2016-01-01 00:00:00

  • Rheological properties of cryptococcal polysaccharide change with fiber size, antibody binding and temperature.

    abstract::Aim:Cryptococcus neoformans is the major agent of cryptococcosis. The main virulence factor is the polysaccharide (PS) capsule. Changes in cryptococcal PS properties have been poorly elucidated. Materials & methods: We analyzed the mechanical properties of secreted PS and intact capsules, using dynamic light scatterin...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: de S Araújo GR,Viana NB,Pontes B,Frases S

    更新日期:2019-07-01 00:00:00

  • Use of high-throughput mass spectrometry to elucidate host-pathogen interactions in Salmonella.

    abstract::Capabilities in mass spectrometry are evolving rapidly, with recent improvements in sensitivity, data analysis and, most important from the standpoint of this review, much higher throughput, allowing analysis of many samples in a single day. This short review describes how these improvements in mass spectrometry can b...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Rodland KD,Adkins JN,Ansong C,Chowdhury S,Manes NP,Shi L,Yoon H,Smith RD,Heffron F

    更新日期:2008-12-01 00:00:00

  • Leprosy as a model of immunity.

    abstract:: Leprosy displays a spectrum of clinical manifestations, such as lepromatous and tuberculoid leprosy, and type I and II lepra reactions, which are thought to be a reflection of the host's immunological response against Mycobacterium leprae. Therefore, differential recognition of M. leprae, as well as its degraded comp...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Degang Y,Nakamura K,Akama T,Ishido Y,Luo Y,Ishii N,Suzuki K

    更新日期:2014-01-01 00:00:00

  • New insights into Prevotella diversity and medical microbiology.

    abstract::In light of recent studies based on cultivation-independent methods, it appears that the diversity of Prevotella in human microbiota is greater than was previously assumed from cultivation-based studies, and that the implication of these bacteria in several human diseases was unrecognized. While some Prevotella taxa w...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Alauzet C,Marchandin H,Lozniewski A

    更新日期:2010-11-01 00:00:00

  • Bacterial triggering of inflammation by intracellular sensors.

    abstract::Recognition of bacterial infection is the first key step to the initiation of an inflammatory response and host defense. Transmembrane proteins of the Toll-like receptor family have long been recognized as key detectors of the extracellular presence of pathogens. Recently, much research has identified a variety of int...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Evans TJ

    更新日期:2009-02-01 00:00:00