Structure-enhanced methods in the development of non-nucleoside inhibitors targeting HIV reverse transcriptase variants.


:Resistance continues to emerge as a leading cause for antiretroviral treatment failure. Several mutations in HIV reverse transcriptase (RT) confer resistance to non-nucleoside inhibitors (NNRTIs), vital components of antiretroviral combination therapies. Since the majority of mutations are located in the NNRTI binding pocket, crystal structures of RT variants in complex with NNRTIs have provided ideas for new drug design strategies. This article reviews the impact of RT crystal structures on the multidisciplinary design and development of new inhibitors with improved resistance profiles.


Future Microbiol


Future microbiology


Frey KM




Has Abstract


2015-01-01 00:00:00












  • Natural history of Bartonella-infecting rodents in light of new knowledge on genomics, diversity and evolution.

    abstract::Among the 33 confirmed Bartonella species to date, more than half are hosted by rodent species, and at least five of them have been involved in human illness causing diverse symptoms including fever, myocarditis, endocarditis, lymphadenitis and hepatitis. In almost all countries, wild rodents are infected by extremely...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Buffet JP,Kosoy M,Vayssier-Taussat M

    更新日期:2013-09-01 00:00:00

  • Micafungin sodium, the second of the echinocandin class of antifungals: theory and practice.

    abstract::Micafungin is a new drug in the echinocandin class and is currently being investigated in Phase III clinical trials. Like other echinocandins, it inhibits 1,3-beta-D-glucan synthesis, thus achieving fungicidal activity against virtually all Candida spp., including those resistant to fluconazole, and fungistatic activi...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Vehreschild JJ,Cornely OA

    更新日期:2006-08-01 00:00:00

  • Progress in the experimental therapy of severe arenaviral infections.

    abstract::A number of viruses in the family Arenaviridae cause severe illness in humans. Lassa virus in West Africa and a number of agents in South America produce hemorrhagic fever in persons exposed to aerosolized excretions of the pathogens' rodent hosts. Because arenaviruses are not transmitted by arthropods, and person-to-...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Gowen BB,Bray M

    更新日期:2011-12-01 00:00:00

  • Endothelial cells and CMV dissemination.

    abstract::Using the murine CMV animal model and the well-established model of Cre-lox-P-mediated green-fluorescence tagging of endothelial cell (EC)-derived mouse CMV to quantify the role of infected ECs in transplantation-associated CMV dissemination (in mice expressing Cre recombinase under the control of either the Tie2 or t...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章


    authors: Gerna G

    更新日期:2012-04-01 00:00:00

  • New perspectives in the management of Pseudomonas aeruginosa infections.

    abstract::Infections with Pseudomonas aeruginosa are a major health problem, especially for immune-compromised and cystic fibrosis patients, owing to the particular drug resistance of the microorganism. The aim of this review is to provide recent insights into strategies under investigation for prevention and therapy of these i...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Savoia D

    更新日期:2014-01-01 00:00:00

  • Comparative evaluation of GeneXpert MTB/RIF and multiplex PCR targeting mpb64 and IS6110 for the diagnosis of pleural TB.

    abstract:AIM:Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test. METHODS:We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Sharma S,Dahiya B,Sreenivas V,Singh N,Raj A,Sheoran A,Yadav A,Gupta KB,Mehta PK

    更新日期:2018-03-01 00:00:00

  • The gut microbiome and the potentials of probiotics: an interview with Simon Gaisford.

    abstract::In this exclusive interview, Simon Gaisford discusses his current research into gut dysbiosis and its possible relevance to disease states, as well as the efficacy of probiotic supplementation and its possible use as treatment. This interview was conducted by Ellen Colvin, Commissioning Editor of Future Microbiology. ...

    journal_title:Future microbiology

    pub_type: 面试


    authors: Gaisford S

    更新日期:2019-03-01 00:00:00

  • Safety, tolerability and pharmacokinetics of AFN-1252 administered as immediate release tablets in healthy subjects.

    abstract:AIMS:AFN-1252 is a novel inhibitor of FabI, an essential enzyme in Staphylococcus spp. This study was undertaken to assess the safety, tolerability and pharmacokinetic properties of AFN-1252, following oral administration in an ascending dose trial. MATERIALS & METHODS:This was a double-blind, randomized, placebo-cont...

    journal_title:Future microbiology

    pub_type: 杂志文章,随机对照试验


    authors: Hafkin B,Kaplan N,Hunt TL

    更新日期:2015-01-01 00:00:00

  • Molecular epidemiology of WU polyomavirus in hospitalized children with acute respiratory tract infection in China.

    abstract:AIM:To explore the molecular epidemiology and clinical characteristics of Washington University polyomavirus (WUPyV) infection in pediatric patients with acute respiratory tract infections in China. MATERIALS & METHODS:A laboratory surveillance was performed to recruit pediatric patients with acute respiratory tract i...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Zhu T,Lu QB,Zhang SY,Wo Y,Zhuang L,Zhang PH,Zhang XA,Wei W,Liu W

    更新日期:2017-05-01 00:00:00

  • Letermovir for prophylaxis of cytomegalovirus manifestations in adult allogeneic hematopoietic stem cell transplant recipients.

    abstract::Cytomegalovirus (CMV) manifestations remain important complications after allogeneic hematopoietic stem cell transplantation (HSCT), even in the current era. Unfortunately, available anti-CMV agents, mainly viral polymerase inhibitors, have a substantial risk of myelosuppression and nephrotoxicity. Letermovir, a novel...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Hecke SV,Calcoen B,Lagrou K,Maertens J

    更新日期:2019-02-01 00:00:00

  • The CRISPR-cas system promotes antimicrobial resistance in Campylobacter jejuni.

    abstract:AIM:The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. MATERIALS & METHODS:The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regula...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Shabbir MA,Wu Q,Shabbir MZ,Sajid A,Ahmed S,Sattar A,Tang Y,Li J,Maan MK,Hao H,Yuan Z

    更新日期:2018-12-01 00:00:00

  • Skin tissue exposure of once- versus twice-daily topical ozenoxacin 2% cream: a Phase I study in healthy volunteers.

    abstract::In this Phase I study, healthy volunteers (n = 24) were randomly allocated to receive either one or two 0.2-g applications per day (12 h apart) of ozenoxacin 2% cream on three different areas of the back for 3 consecutive days. Ozenoxacin concentrations were measured in tape stripping samples (from the stratum corneum...

    journal_title:Future microbiology

    pub_type: 杂志文章,随机对照试验


    authors: Gropper S,Albareda N,Santos B,Febbraro S

    更新日期:2014-01-01 00:00:00

  • Probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis.

    abstract:AIMS:To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression. MATERIALS & METHODS:The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were inte...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Chen ZF,Ai LY,Wang JL,Ren LL,Yu YN,Xu J,Chen HY,Yu J,Li M,Qin WX,Ma X,Shen N,Chen YX,Hong J,Fang JY

    更新日期:2015-01-01 00:00:00

  • The role of bacteriophages in periodontal health and disease.

    abstract::The human periodontium health is commonly compromised by chronic inflammatory conditions and has become a major public health concern. Dental plaque, the precursor of periodontal disease, is a complex biofilm consisting mainly of bacteria, but also archaea, protozoa, fungi and viruses. Viruses that specifically infect...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Pinto G,Silva MD,Peddey M,Sillankorva S,Azeredo J

    更新日期:2016-10-01 00:00:00

  • Comparative analysis between biofilm formation and gene expression in Staphylococcus epidermidis isolates.

    abstract:AIM:To understand the relationship between ica, aap and bhp gene expression and the implications in biofilm formation in selected clinical and commensal Staphylococcus epidermidis isolates. MATERIAL & METHODS:Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, biofilm spati...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Freitas AI,Lopes N,Oliveira F,Brás S,França Â,Vasconcelos C,Vilanova M,Cerca N

    更新日期:2018-03-01 00:00:00

  • N-linked glycosylation in bacteria: an unexpected application.

    abstract::Traditionally, glycoproteins have been considered the exclusive property of eukaryotes and archaea, but it is now evident that glycoproteins are found in all domains of life. In recent years N-linked glycosylation among some epsilon-proteobacteria has emerged as a new and exciting research area and represents a useful...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Langdon RH,Cuccui J,Wren BW

    更新日期:2009-05-01 00:00:00

  • Bacterial triggering of inflammation by intracellular sensors.

    abstract::Recognition of bacterial infection is the first key step to the initiation of an inflammatory response and host defense. Transmembrane proteins of the Toll-like receptor family have long been recognized as key detectors of the extracellular presence of pathogens. Recently, much research has identified a variety of int...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Evans TJ

    更新日期:2009-02-01 00:00:00

  • The contribution of genomics to the study of Q fever.

    abstract::Coxiella burnetii is the etiological agent of Q fever, a worldwide zoonosis that can result in large outbreaks. The birth of genomics and sequencing of C. burnetii strains has revolutionized many fields of study of this infection. Accurate genotyping methods and comparative genomic analysis have enabled description of...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: D'Amato F,Eldin C,Raoult D

    更新日期:2016-01-01 00:00:00

  • Cryptococcus gattii infections: contemporary aspects of epidemiology, clinical manifestations and management of infection.

    abstract::Cryptococcus gattii is an important primary and opportunistic pathogen, predominantly causing meningoencephalitis and pulmonary disease with substantial mortality. Initially considered geographically restricted to immune-competent, highly exposed individuals in the tropics, an apparent epidemic in North America has le...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: McMullan BJ,Sorrell TC,Chen SC

    更新日期:2013-12-01 00:00:00

  • Repertoire of human breast and milk microbiota: a systematic review.

    abstract::Breastfeeding is a major determinant of human health. Breast milk is not sterile and ecological large-scale sequencing methods have revealed an unsuspected microbial diversity that plays an important role. However, microbiological analysis at the species level has been neglected while it is a prerequisite before under...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Togo A,Dufour JC,Lagier JC,Dubourg G,Raoult D,Million M

    更新日期:2019-05-01 00:00:00

  • Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS.

    abstract:AIM:We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. MATERIALS & METHODS:Staphylococcus argenteus was identified based on negative crtM gene and presence of specific s...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Chen SY,Lee H,Teng SH,Wang XM,Lee TF,Huang YC,Liao CH,Teng LJ,Hsueh PR

    更新日期:2018-07-01 00:00:00

  • Evaluation of the antifungal activity in vitro of midazolam against fluconazole-resistant Candida spp. isolates.

    abstract::Aim: The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical Candida spp. in planktonic and biofilm form. Materials & methods: The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests we...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Holanda MA,da Silva CR,de A Neto JB,do Av Sá LG,do Nascimento FB,Barroso DD,da Silva LJ,Cândido TM,Leitão AC,Barbosa AD,de Moraes MO,Cc B,Júnior HVN

    更新日期:2021-01-01 00:00:00

  • Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.

    abstract:AIM:Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. MATERIALS & METHODS:Available information from 929 surgical patients was obtained from local health auth...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Leão SC,Viana-Niero C,Matsumoto CK,Lima KV,Lopes ML,Palaci M,Hadad DJ,Vinhas S,Duarte RS,Lourenço MC,Kipnis A,das Neves ZC,Gabardo BM,Ribeiro MO,Baethgen L,de Assis DB,Madalosso G,Chimara E,Dalcolmo MP

    更新日期:2010-06-01 00:00:00

  • Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing.

    abstract:AIM:To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. METHODS:95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. RESULTS:The isolates showed a high diversity (IOD 0.964) w...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Garcia-Nuñez M,Quero S,Pedro-Botet ML,Barrabeig I,Avarez J,Campoy I,Sala MR,Parraga-Niño N,Minguell S,Caylà J,Mateu L,Sabria M

    更新日期:2016-07-01 00:00:00

  • A multifaceted 'omics' approach for addressing the challenge of antimicrobial resistance.

    abstract::The inappropriate use of antibiotics has severe global health and economic consequences, including the emergence of antibiotic-resistant bacteria. A major driver of antibiotic misuse is the inability to accurately distinguish between bacterial and viral infections based on currently available diagnostic solutions. A m...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Cohen A,Bont L,Engelhard D,Moore E,Fernández D,Kreisberg-Greenblatt R,Oved K,Eden E,Hays JP

    更新日期:2015-01-01 00:00:00

  • Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in Greece: an extended review (2000-2015).

    abstract::Carbapenem-resistant Acinetobacter baumannii (CRAB) is endemic in Greece. CRAB initially emerged in 2000 and since then, carbapenemases still have a crucial role in CRAB appearance, except for a few cases resulting from efflux pump or outer-membrane protein mechanisms. OXA-type carbapenemases present the highest preva...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Karampatakis T,Antachopoulos C,Tsakris A,Roilides E

    更新日期:2017-07-01 00:00:00

  • The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus.

    abstract::Aim: Antibiotic resistance in Mycobacterium abscessus renders treatment poorly effective. Despite erm(41)-gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in erm(41) induction. We determine whether this is the case. Methods:M. a...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Schildkraut JA,Pennings LJ,Ruth MM,de Brouwer AP,Wertheim HF,Hoefsloot W,de Jong A,van Ingen J

    更新日期:2019-06-01 00:00:00

  • Manipulation of the bluetongue virus tubules for immunogen delivery.

    abstract::A multidisplay vaccine delivery system has been developed that is nonreplicating and has a protein-based particulate structure. The structure is composed of helical tubules comprising multiple copies of a single nonstructural (NS) protein 1 of bluetongue virus. The helical assemblies present the C terminus of the prot...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Murphy A,Roy P

    更新日期:2008-06-01 00:00:00

  • Role of catalase overproduction in drug resistance and virulence in Candida albicans.

    abstract:AIM:To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS:Strains overproducing the CAT1 gene were constructed. RESULTS:Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen spe...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Román E,Prieto D,Martin R,Correia I,Mesa Arango AC,Alonso-Monge R,Zaragoza O,Pla J

    更新日期:2016-10-01 00:00:00

  • Retapamulin: a semisynthetic pleuromutilin compound for topical treatment of skin infections in adults and children.

    abstract::Retapamulin is a semisynthetic pleuromutilin compound with in vitroactivity against Gram-positive bacteria, no cross-resistance to other classes of antimicrobial agents in current use and a low potential for development of resistance. A 1% ointment formulation has been developed for clinical use, and a placebo-control...

    journal_title:Future microbiology

    pub_type: 临床试验,杂志文章


    authors: Jacobs MR

    更新日期:2007-12-01 00:00:00