Abstract:
:Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on mammary carcinoma cell morphology and phenotype have not been thoroughly characterized. Here, we evaluated fundamental effects of environmental acidification on mammary carcinoma cells in standard two-dimensional cultures and three-dimensional spheroids. Acidification decreased overall mammary carcinoma cell viability, while increasing their resistance to the anthracycline doxorubicin. Environmental acidification also increased extracellular vesicle production by mammary carcinoma cells. Conditioned media containing these vesicles appeared to increase fibroblast motility. Acidification also increased mammary carcinoma cell motility when cultured with fibroblasts in spheroids. Taken together, results from this study suggest that environmental acidification induces drug resistance and extracellular vesicle production by mammary carcinoma cells that promote tumor expansion.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ralph ACL,Valadão IC,Cardoso EC,Martins VR,Oliveira LMS,Bevilacqua EMAF,Geraldo MV,Jaeger RG,Goldberg GS,Freitas VMdoi
10.1038/s41598-020-78989-6subject
Has Abstractpub_date
2020-12-15 00:00:00pages
21959issue
1issn
2045-2322pii
10.1038/s41598-020-78989-6journal_volume
10pub_type
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