Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro.

Abstract:

:Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on mammary carcinoma cell morphology and phenotype have not been thoroughly characterized. Here, we evaluated fundamental effects of environmental acidification on mammary carcinoma cells in standard two-dimensional cultures and three-dimensional spheroids. Acidification decreased overall mammary carcinoma cell viability, while increasing their resistance to the anthracycline doxorubicin. Environmental acidification also increased extracellular vesicle production by mammary carcinoma cells. Conditioned media containing these vesicles appeared to increase fibroblast motility. Acidification also increased mammary carcinoma cell motility when cultured with fibroblasts in spheroids. Taken together, results from this study suggest that environmental acidification induces drug resistance and extracellular vesicle production by mammary carcinoma cells that promote tumor expansion.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ralph ACL,Valadão IC,Cardoso EC,Martins VR,Oliveira LMS,Bevilacqua EMAF,Geraldo MV,Jaeger RG,Goldberg GS,Freitas VM

doi

10.1038/s41598-020-78989-6

subject

Has Abstract

pub_date

2020-12-15 00:00:00

pages

21959

issue

1

issn

2045-2322

pii

10.1038/s41598-020-78989-6

journal_volume

10

pub_type

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